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Bacillus licheniformis strain and application thereof

A technology of Bacillus licheniformis and strains, applied in the directions of bacteria, enzymes, biochemical equipment and methods, etc., can solve the problems of long production cycle and increased production cost, etc.

Active Publication Date: 2015-07-22
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

Not to mention the large loss of enzymes in this process, the use of this production procedure and the long production cycle also greatly increase the production cost of the enterprise

Method used

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  • Bacillus licheniformis strain and application thereof
  • Bacillus licheniformis strain and application thereof
  • Bacillus licheniformis strain and application thereof

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Embodiment Construction

[0022] A bacillus licheniformis bacterial strain, described bacterial strain is bacillus licheniformis bacterial strain BL14 (Bacillus licheniformis), on February 28, 2014, was preserved in China Microorganism Culture Preservation Management Committee General Microorganism Center, preservation number is CCMCC NO.8865.

[0023] The Bacillus licheniformis strain can be applied to the treatment of tofu wastewater, and also produces endoglucanase and xylanase compound enzyme preparations. The specific method is as follows: collect the discarded tofu wastewater in the production of tofu, and adjust the pH of the tofu wastewater to 7.0, placed in a fermenter for 121°C autoclaving for 25min, the Bacillus licheniformis strain BL14 was sterilized with 10 6 The inoculum amount of cfu / mL was inoculated in a fermenter, aerated, and cultured at 33°C for 3 days, and the tofu wastewater was fermented into a compound enzyme liquid containing endoglucanase and xylanase.

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Abstract

The invention relates to a bacillus licheniformis strain and application thereof. The strain is a bacillus licheniformis strain BL14 (Bacillus licheniformis) which is preserved in CCMCC on 28th, February, 2014, wherein the preservation number is CCMCC NO. 8865. The application of the bacillus licheniformis strain comprises the following steps: collecting bean curd wastewater; adjusting the pH to 7.0; putting at 121 DEG C and autoclaving for 25 minutes; inoculating the bacillus licheniformis strain BL14 to the bean curd wastewater according to an inoculation quantity of 106cuf / mL; aerating; culturing for 3 days at 33 DEG C and fermenting the bean curd wastewater to a compound enzymatic liquid. The bacillus licheniformis can ferment and grow by taking the bean curd wastewater as a unique nutritional substance and produce an enzymatic liquid of endoglucanase and xylanase, so that a novel fermenting bacterial source is provided for producing endoglucanase and xylanase preparations.

Description

technical field [0001] The invention specifically relates to a bacillus licheniformis bacterial strain and application thereof. Background technique [0002] In order to make full use of resources and save costs, substances with high cellulose and hemicellulose content, such as rice bran, wheat flour, cottonseed meal, straw powder, pasture, etc., are often used as raw materials to replace high-priced corn, soybeans, wheat and other substances. However, a high proportion of crude fiber diets not only cannot be directly absorbed and utilized by livestock and poultry, but also easily cause various problems such as diarrhea and slow growth of livestock and poultry. In order to improve feed quality, feed mills and farms have fully realized the importance of adding enzymes to feed, especially non-digestive enzymes such as cellulase and hemicellulase that cannot be synthesized by animals themselves. [0003] The main components of plant cell walls are composed of cellulose, hemice...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12R1/10
Inventor 官雪芳徐庆贤林斌钱蕾
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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