The invention discloses a limitation method of a Web server concurrent number, relating to the field of information technologies. In a three-tier system framework, the Web server accesses to a database by a connection pool; when user requests have certain large statics or inquiry, the connection pool is gradually used up by the requests, thus the whole system stops responding. In the limitation method of a Web server concurrent number, after transferring client-side information to the functional module of each service, the database connection pool is accessed by an inquiry connection module; the inquiry connection module is divided into a large inquiry connection module and a common inquiry connection module, wherein the large inquiry connection module has the maximum total concurrent number; when a user side submits a large inquiry request, if the request number is smaller than the maximum total concurrent number, one database connection is obtained from the database connection pool, and a database access request is submitted to the database server by the connection; otherwise, the user is prompted to wait. The maximum total concurrent number is set to limit large inquiry connection amount and ensure the response capability of common inquiry.