The invention belongs to the field of biomedicine, and particularly relates to a compound containing cyclohydroxamic acid as well as a preparation method and an application of same. The structural general formula of the compound containing cyclohydroxamic acid is shown as the specification, wherein R is one of F, Cl, Br, I,-CH3, -OCH3, -SCH3, acetyl group and cyanate radical; R1 is H, -CH3, -(CH2)nCH3, a benzene ring and an aromatic ring that has any one of F, Cl, Br, I, O, OH, -CH3, -OCH3, and cyano group; wherein the n is an integer from 1 to 8; and R2 is one of a C1-C14 chain substituent group, hydrogen, allyl, alkenebutyl, benzyl, aromatic ring, and one of benzyl group and sulphonyl group having a substituent group. According to the compound, acinetobacter baumannii infection can be reduced by inhibiting a biological membrane, the occurrence probability of infectious diseases is reduced from the source, and side effects are few. The compound also has good exopolysaccharide inhibition capability and is non-toxic.