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Building method for drug resistance baumanii infection caenorhabditis elegans medicine screening model and application thereof

A technology of Acinetobacter baumannii and Caenorhabditis elegans, which is applied in animal husbandry and other fields, can solve problems such as difficulty in infection treatment, achieve the effects of saving medical resources, reducing mortality and hospitalization time, and reducing medical costs

Inactive Publication Date: 2018-11-02
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

Acinetobacter baumannii has become one of the most common Gram-negative bacilli among inpatients in major hospitals. In recent years, the detection rate of Acinetobacter baumannii has been increasing, which has caused great difficulties in the treatment of infections.

Method used

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  • Building method for drug resistance baumanii infection caenorhabditis elegans medicine screening model and application thereof
  • Building method for drug resistance baumanii infection caenorhabditis elegans medicine screening model and application thereof
  • Building method for drug resistance baumanii infection caenorhabditis elegans medicine screening model and application thereof

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Embodiment Construction

[0025] Specific embodiments of the present invention will be described below.

[0026] 1. Materials and methods

[0027] 1.1 Identification and collection of strains: Pan-drug-resistant Acinetobacter baumannii was clinically isolated from the body fluid samples of patients infected with drug-resistant bacteria included in the study, and the strains were identified by multiple PCR and colloidal gold methods, and used when necessary Strains were accurately identified by direct sequencing. The strains were collected, cultured and preserved. N2 wild-type Caenorhabditis elegans (C.elegans, Bristolstrain N2) and E.coli OP50 were donated by the Institute of Genetics and Development, Chinese Academy of Sciences.

[0028] 1.2 In vitro drug susceptibility test: The in vitro drug susceptibility test of patients adopts the disk method and dilution method, and the drug used is the conventional in vitro drug susceptibility test program of Acinetobacter baumannii.

[0029] 1.3 Preparation...

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Abstract

The invention provides a building method for a drug resistance baumanii infection caenorhabditis elegans medicine screening model. The method comprises the steps of 1, separating out pan-drug resistant acine-tobacter baumannii; 2, separating out eggs from pregnant adult insects, performing incubation, transferring young adults on an E.coli lawn to continue growing in an incubator, after insect bodies are eluted, transferring the insect bodies to a lawn of pan-drug resistant acine-tobacter baumannii, and performing cultivation for 8-24 h in the incubator for infection; 3, washing down the insect bodies, placing the insect bodies on a culture plate containing 5-FU, performing cultivation overnight, obtaining synchronous caenorhabditis elegans, and building a drug screening model. Nematodes are selected as an experimental subject, a drug sensitivity experiment is performed on the pan-drug resistant acine-tobacter baumannii, the result is compared with an in vitro chemosensitivity assay result, the clinic reference value is obtained, and the omission in traditional in-vitro susceptibility testing can be compensated for.

Description

technical field [0001] The present invention relates to a method for establishing a drug screening model of Caenorhabditis elegans infected by drug-resistant Acinetobacter baumannii, and also relates to a drug obtained by using the method for establishing a drug screening model of drug-resistant Acinetobacter baumannii infected Caenorhabditis elegans Application of screening models. Background technique [0002] Infectious diseases have always been one of the major public health problems in our country. Due to the long-term and extensive use of broad-spectrum antibiotics, many pathogenic bacteria have surprisingly developed resistance to antibiotics. In recent years, nosocomial infections have been on the rise, and some nosocomial infections are refractory and multi-drug resistant, seriously threatening the health and life safety of patients. Acinetobacter baumannii has become one of the most common Gram-negative bacilli among inpatients in major hospitals. In recent years,...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 徐阿晶蒋兴浩张健黄晓会卜书红陈其琪费爱华
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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