434 results about "Drugs sensitivity" patented technology

This invention features methods of identifying genetic alterations that can modulate cancer cells' sensitivity to an anti-cancer drug. Information on such genetic alterations can be used to predict cancer therapeutic outcomes and to stratify patient populations to maximize therapeutic efficacy.

The invention provides the components of in vivo and in vitro systems and methods which use them to study the effects of altered expression of a gene activity, such as the human akt, bcl-2, eIF4E or PTEN activities, on the descendants of stem cells that have been engineered to give rise to hematopoietic tumorigenic or tumor cells, such as lymphomas, with a high frequency. The present invention provides vectors, cells and mammals, and methods which in part depend on such products, useful for understanding tumorigenesis and its treatments, and in particular, for identifying and studying inhibitors and activators associated with tumor cell growth and growth inhibition, cell death through apoptotic pathways, and changes in apoptotic pathway components that affect drug sensitivity and resistance in tumorigenic cells. Methods for identifying molecular targets for drug screening, identifying interacting gene activities, for identifying therapeutic treatments and for identifying candidates for new therapeutic treatments are provided.

The invention belongs to the technical field of helicobacter pylori (Hp) examination and discloses a kit for fast culture, identification and a drug sensitivity test of Hp and an examination method therefor. The kit comprises a first culture tube, an identification reagent device and a drug sensitivity test unit. Through the first culture tube of Hp, bedside inoculation of a specimen is realized and the traditional sample transport and preservation processes are avoided; ordinary thermostat culture is realized and dependence on a CO2 incubator is eliminated; an Hp growth result is represented by a color change so that a result can be determined accurately by laypeople; through the combination of a growth promoting agent and a growth indicator, culture time is shortened and fast culture of Hp is realized; an Hp identification reagent, fast combination identification of Hp is realized; and through the drug sensitivity test unit of Hp, fast and accurate examination of drug sensitivity of Hp is realized. The kit and the examination method therefor can realize fast and accurate examination of Hp.

The invention belongs to the field of biological medicines and relates to an application of combination of a Caspase activator and an oncolytic virus in preparation of an antitumor drug. The condition that the antitumor effect of the oncolytic virus can be improved with the Caspase activator is discovered for the first time, the combination of the Caspase activator and the oncolytic virus has a quite high synergistic effect, and an effective therapeutic schedule is provided for oncotherapy with low drug sensitivity.

Described herein is a network biology approach useful for the identification of multiple therapeutic targets, which can be targeted simultaneously using an agent (or a plurality of agents) to modulate cellular phenotypes, or in combination with pharmaceutical compounds to improve drug sensitivity and/or reduce drug doses to maintain efficacy while minimizing side effects. The preferred approach disclosed herein relies on first identifying the mediators of a condition of interest, and second, selecting gene combinations that are in competing/parallel pathways as targets for combination therapy.

The invention provides a breast cancer multidrug-resistant cell strain constructed by virtue of epirubicin induction, wherein the breast cancer multidrug-resistant cell strain has a collection number of CCTCC C201439. The invention further provides a method for constructing the breast cancer multidrug-resistant cell strain. According to the invention, an MDA-MB-231/EPI drug-resistant strain is successfully established, wherein a drug-resistant index is 26.27 times, and obvious cross resistance is produced for taxol, etoposide and cis-platinum. A drug-resistant tumor cell model is provided for the following related researches so as to research drug-resistant tumor cell morphology and biological characteristics, research a tumor multidrug resistance mechanism, analyze sensitivity to chemotherapeutic drugs as well as screen chemotherapeutic drugs, develop tumor drug resistant reverse drugs and research and develop a more effective tumor therapeutic method, and the like.

The invention discloses a bacteria drug-resistance detection system and an operation method thereof. The system comprises a drug sensitive test inoculation instrument, drug sensitive test image acquisition conversion equipment, a drug sensitivity detection kit and a bacterial strain culture device. A medium is arranged in the drug sensitivity detection kit. The drug sensitive test inoculation instrument is used for inoculating a bacterial strain into the drug sensitivity detection kit. The bacterial strain culture device provides a growth environment for the bacterial strain in the drug sensitivity detection kit. The drug sensitive test image acquisition conversion equipment is used for acquiring and converting images of the bacterial strain in the drug sensitivity detection kit. Management software is arranged in the drug sensitive test image acquisition conversion equipment, and the management software is connected to the network. With combination of online and offline, clinical medication is directly guided. The system meets on-site detection requirements, is convenient for data analysis and decision-making for a farm, a veterinary medicines factory and an administrative department, is suitable for current high-level drug resistance situation in our country, and satisfies the need of quantitative networked monitoring of drug resistance. A broth dilution method and an agar dilution method are both considered. The system meets different requirements of large-scale monitoring and scattered sample detection.

Based on drug sensitivity data and extensive gene expression data, a model was constructed by multivariate analysis with the partial least squares method type 1. Further, the model was optimized using modeling power and genetic algorithm. Thereby, the degree of contribution of the respective genes to drug sensitivity was determined to select genes with a high degree of contribution. In addition, the levels of gene expression in specimens were analyzed, and then the drug sensitivity was predicted based on the model. The predicted values agreed well with those drug sensitivity values determined experimentally. The drug sensitivity-predicting method provided by the present invention enables assessment of the effectiveness of a drug prior to administration using small quantities of specimens associated with diseases such as cancer. Since this enables the selection of the most suitable drug for each patient, the present invention is very useful in improving a patient's quality of life (QOL).

The invention relates to an anti-tumor drug screening kit based on a three-dimensional micro-tissue level and an application method thereof. The anti-tumor drug screening kit comprises a three-dimensional cell culture material, a tissue cell dispersion reagent, a proteinase inhibitor, a basic cell culture medium, blood serum, antibiotics, a balance salt solution, a cell activity detection reagentand a cell filter screen. The application method comprises the following steps: dispersing tumor tissues of a patient to obtain tissue blocks, tissue fragments, cell aggregates and/or dispersed singlecells; then culturing the dispersed tumor tissues of the patient and a cell sample through a three-dimensional culture method and enabling the cells to grow in a three-dimensional space to form a spherical structure or an irregular three-dimensional structure with a stereoscopic shape; finally, carrying out a drug sensitivity test on tumor micro-tissues obtained by three-dimensional culture. Theanti-tumor drug screening kit based on the three-dimensional micro-tissue level is simple to operate and is suitable for the drug sensitivity tests of various solid tumors.

The invention discloses a novel application of honokiol and fluconazole in antifungal cooperation. According to the invention, the fungi are candida albicans. According to the invention, a standard macrodilution drug sensitivity experiment recommended by CLSI is adopted, in vitro sensitivity of honokiol towards clinically separated drug resistant candida albicans is evaluated with systematic methods such as a checkerboard macrodilution method and a time-kill curve method. Also, an in vitro synergistic effect of honokiol and fluconazole in clinic drug resistant fungi resistance is evaluated. Further, with a mice infection and treatment experiment, an in vivo synergistic effect of honokiol and fluconazole in clinic drug resistant fungi resistance is evaluated.

The invention relates to a fast drug sensitivity detection kit for vibrio, which can be applied to an aquiculture site. According to the invention, the detection kit comprises a detection plate, a thallus collection tool, bacterium enrichment liquid, a drug sensitivity detection culture medium, a turbidimetry tube, an inoculation burette and a colorimetric card, wherein the detection plate comprises inspection holes enveloping drugs and color indicators for drug sensitivity detection, and the color indicators are Alamar Blue. According to the invention, fish peptone, Ca2+ and Mg2+ are added based on the MH culture medium and the concentration of NaCl is adjusted, and the culture medium is used as the bacterium enrichment liquid of vibrio, which can effectively promote the growth of the vibrio and enables the bacterium enrichment liquid to reach the required concentration in a short time, so as to shorten the time of drug sensitivity detection. At the same time, a blue dye, namely the Alamar Blue, which is safe and nontoxic to the cells is used; the Alamar Blue has no toxic effect on cells, and can be enveloped in the micropores of the drug sensitivity detection plate directly without influencing the growth of the bacteria, so that the detection operation is simpler and more convenient and quick; and the proliferation dynamic of the bacteria can be continuously monitored.

A method is disclosed for assaying the sensitivity of neoplastic tissue to therapeutic agents, and in particular, for the quantification of pro-apoptotic marker mRNA expression in cells obtained from thinly-sliced living tumor tissue in such methods. The method may comprise ascertaining a particular apoptosis marker mRNA for an individual tumor or tumor type as well as exposure of thin-sliced live cancer tissues from the individual tumor to candidate chemotherapeutic drug regimes in vitro, followed by an assessment of the level of the marker mRNA in the tissue.

The invention relates to a preparation method for a drug sensitivity kit and a drug sensitivity detection method, which are applied in the field of aquiculture. The drug sensitivity kit comprises a centrifuge tube with enriched bacterial culture solution, a culture dish with solid culture medium and a drug sensitivity drug storage ring. According to the invention, drug sensitivity detection is carried out on suscepts and water samples and the drug sensitivity drug storage rings are observed after the detection; drugs in drug sensitivity plasters where a transparent area appears can all be used as alternative drugs, and the drug in a drug sensitivity plaster where a big clear area appears can be used as a preferable drug; therefore a most appropriate antibiotic is selected out. Using a drug based on a correct diagnosis can avoid abuse of fish drugs and particularly abuse of antibiotics, and prevent medicinal residues from remaining in aquatic products. It only needs 12 hours to complete the preparation of the drug sensitivity kit; thus selection of drugs and corresponding prevention and treatment can be carried out in a same day as the preparation. The invention has the advantages of simple and convenient operation and low cost, is applicable to selection of drugs for controlling bacterial diseases in basic level of aquiculture, and plays a positive role in protecting ecological environment for aquiculture.

The invention relates to the field of biotechnology, in particular to an organoid culture medium and an organoid culture method. The organoid culture medium comprises a base medium, hydrothorax supernatant, enzyme hydrolyzed casein, hydrocortisone, l-glutamic acid, insulin, transferrin, cholera viruses, Y-27632, EGF, amphotericin B, penicillin and streptomycin. The organoid culture method includesperforming two-dimensional culture on tumor cells, and performing three-dimensional culture on the cultured cells to form organoid tissue. The medium formula can promote the rapid proliferation of tumor tissue while avoiding hypoxia injuries, and the culture method can greatly shorten the time required for a subsequent drug sensitivity test; the accuracy of the drug sensitivity test is improved,and the test cost is reduced.

The present invention comprises a treatment approach based on gene set-expression signatures that systematically connects a sample to a profile from a reference database to extrapolate the most effective therapeutic agent. Further disclosed are methods to optimize combination treatments.

The invention provides an accompanying diagnosis model based on a PDX or PDC model drug sensitivity experiment and multi-omics detection analysis, a construction method and application. The method comprises four modules of construction of a human tumor cell line model and an animal transplantation model, a drug sensitivity experiment based on the human tumor cell line model and the animal transplantation model, acquisition of multi-omics data of a human tumor sample, and mining analysis of drug sensitivity phenotype-multi-omics data. The invention provides an implementation thought and a key method of the four modules, and reasonable design of correlation and matching among the four modules, and a whole set of integration strategy of model construction, drug sensitivity experiments, omicsdata acquisition, data integration and data mining analysis is formed. According to the method, the limitation of technical means of an existing accompanying diagnosis scheme research and developmentstrategy in the links of sample planning, data acquisition, data analysis and the like is overcome, accompanying diagnosis scheme research and development can be carried out more flexibly and more widely, and meanwhile mechanism interpretation clues related to markers are obtained.

It is herewith provided methods of obtaining circulating cancer cells (CCCs)-enriched cellular populations based on the removal of CD45-positive cells from an apheresis product. It is also provided methods of obtaining circulating stem cells (CSCs)-enriched cellular population from a CCCs-enriched cellular population based on a selection either using the specific cellular markers or through culture. It is further provided methods of obtaining circulating tumor cells (CTCs)-enriched cellular population from a CCCs-enriched cellular population based on a selection using the specific cellular markers. It is also provided screening assays for the selection of (chemo)therapeutic agent as well as personalized medicine application based on drug sensitivity/resistance or cancer markers.

The invention discloses a drug sensitivity prediction method based on cell line and drug similarity networks, which comprises the steps of: constructing the drug similarity network, the cell line similarity network and a drug-cell line relationship network; respectively acquiring a drug adjacent matrix, a cell line adjacent matrix and a drug-cell line relationship initial matrix according to the drug similarity network, the cell line similarity network and the drug-cell line relationship network; and based on the drug adjacent matrix, the cell line adjacent matrix and the drug-cell line relationship initial matrix, obtaining a drug-cell line drug sensitivity prediction matrix by adopting an unbalanced double random walk algorithm, wherein each element in the drug-cell line drug sensitivityprediction matrix obtained after walk is completed by adopting an unbalanced double random walk formula is a predicted sensitivity value of a corresponding drug for a cell line. According to the invention, characteristics of the drug similarity network and the cell line similarity network are sufficiently considered, so that reliability of a drug sensitivity prediction result is improved.

Diagnostic and therapeutic methods of cancer treatment and prevention using metabolic profiling compounds that contain [1,2-¹³C₂]-D-glucose, and kits for using such metabolic profiling compounds.