Application method of phages for treatment of pan-drug resistant Acinetobacter baumannii infection in burn patients

A technology of Acinetobacter baumannii and application method, applied in the application field of bacteriophage, can solve the problems of low efficiency, lack of pertinence, disordered library construction and the like

Inactive Publication Date: 2018-05-08
SOUTHWEST HOSPITAL OF CHONGQING
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] However, the existing technical solutions are blind screening of phages, which lead to chaotic library construction, lack of pertinence, and low efficiency

Method used

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  • Application method of phages for treatment of pan-drug resistant Acinetobacter baumannii infection in burn patients
  • Application method of phages for treatment of pan-drug resistant Acinetobacter baumannii infection in burn patients

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Embodiment

[0037] Embodiment: a kind of application method of phage in the treatment of pan-drug-resistant Acinetobacter baumannii infection in burns, comprising the following steps:

[0038] 1. Epidemiological investigation of Acinetobacter baumannii infection in burn wards in my country, collecting popular strains;

[0039] (1) Investigation on the infection of Acinetobacter baumannii in 15 domestic burn wards: A cross-sectional survey was conducted on the burn wards of 15 hospitals in 10 cities including Beijing, Shanghai, Guangzhou, Chongqing, etc. The popular Acinetobacter baumannii, combined biochemical methods (VITEKGNI+cards) and molecular biology methods (16SrRNA gene sequencing and OXA-51 gene detection) to verify whether each strain of bacteria is Acinetobacter baumannii. According to the results of the drug susceptibility test, combined with the definition of pan-drug-resistant Acinetobacter baumannii, strains of pan-drug-resistant Acinetobacter baumannii were screened out. ...

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Abstract

The invention relates to an application method of phages for treatment of pan-drug resistant Acinetobacter baumannii infection in burn patients. Firstly, according to epidemiological investigation ofAcinetobacter baumannii infection in domestic burn patients, collection of epidemic strains is carried out; secondly, the collected pan-drug resistant Acinetobacter baumannii are subjected to MLST typing; thirdly, according to step two, the phages of each MLST type of Acinetobacter baumannii are separated, a phage library of pan-drug resistant Acinetobacter baumannii is constructed; fourthly, thetreatment effect of the phage library on pan-drug resistant Acinetobacter baumannii infection is tested by animal experiments, more specifically, an effective phage library is obtained. Different fromprevious blind separation of phages, we base on bacterial transformation and MLST typing, targeted separation of phages is carried out, in this way, phages can be separated more quickly and effectively, a phage library reaching a certain breaking-bacteria spectrum is constructed.

Description

technical field [0001] The invention relates to the technical field of phage application, in particular to an application method of phage used in the treatment of pan-drug-resistant Acinetobacter baumannii infection in burns. Background technique [0002] There are more than 1,000 ST subtypes of Acinetobacter baumannii, and each ST subtype corresponds to multiple phages. In order to accurately and effectively treat Acinetobacter baumannii infection, it is considered to isolate phage more precisely for its specific subtype. [0003] However, the existing technical solutions are all blind screening of phages, which lead to chaotic library construction, lack of pertinence, and low efficiency. This technical solution has high specificity when screening phages. First, the collected clinical Acinetobacter baumannii is typed by the internationally recognized MLST to determine the subtype of Acinetobacter baumannii. The phages were then screened against this subtype. After screen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/76A61P31/04C12N7/00
CPCA61K35/76C12N7/00C12N2795/00051
Inventor 彭毅志黄广涛杨子晨龚雅利
Owner SOUTHWEST HOSPITAL OF CHONGQING
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