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34results about How to "Unaffected by environmental conditions" patented technology
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Fragrant immobilized granular activation-free chlorine dioxide air purifying agent
InactiveCN105123748AUnaffected by environmental conditionsEliminate odorBiocideDispersed particle separationMaterials preparationChlorine dioxide
The invention discloses a fragrant immobilized granular activation-free chlorine dioxide air purifying agent and belongs to the field of environmental purification. The air purifying agent includes following components: a solidifying agent, a chlorine dioxide generating substance, spices, a coloring agent, a slow-releasing agent, a filling agent and the like. A preparation method includes the steps of material preparation, grinding, drying, sieving, mixing and moulding. The air purifying agent can effectively kill bacteria, virus, chemical toxins and residual pesticides and the like harmful substances. The air purifying agent is convenient to use, is simple in operation, is significant in effects, is long-acting, safe and long-shelf-life, and has wide application prospect in the fields of household environments, medical care and health care, hygiene and epidemic prevention and the like.
Owner:ZHUAN BEIJING BIOTECH DEV CO LTD
Primer sequence for judging cucumber powdery mildew resistance and judging method therefor
InactiveCN1482132AUnaffected by environmental conditionsSugar derivativesMicrobiological testing/measurementAgricultural scienceGel electrophoresis
The present invention discloses one kind of primer series for identifying cucumber powdery mildew and its identification method. The primer series consists of upstream primer 5'-CAG TAA ATG AAA GAA AAG AAG-3' and downstream primer 5'-ATA CAT AGC CAT ACA AAA AT-3'. The identification method of cucumber powdery mildew with the primer series includes the following steps: extracting cucumber genomeDNA; PCR proliferation; gel electrophoresis analysis of the PCR proliferation product; and identifying the resistance of cucumber powdery mildew according to the relative position of the identified sample on gel strip. The said method has its result possess coincidence rate with field detection result up to 94 %, and is fast, accurate and not affected by environment condition, so that it has high application value in screening cucumber powdery mildew resistance.
Owner:TIANJIN RES INST OF VEGETABLE +1
Cucumber male sterility gene related SNP (Single Nucleotide Polymorphism) marker and application
ActiveCN105420408APrecise positioningLow costMicrobiological testing/measurementDNA/RNA fragmentationRe sequencingAgricultural science
The invention discloses a cucumber male sterility gene related SNP (Single Nucleotide Polymorphism) marking method and application. The marking method comprises the steps of taking a YL-5 male sterile line as a female parent to hybridize with a distant related variety D37-1, so as to obtain a hybrid F1, performing selfing by the hybrid F1 to obtain an F2 population, and two phenotypes of male sterility and male fertility exist in an F2 segregation population, then utilizing a method of combination of re-sequencing and BSA, screening an SNP locus unrelated with the male sterility, and developing a male sterility gene related molecular marker. The marking method disclosed by the invention has the advantages of short period, low cost and high efficiency. Meanwhile, an important development approach is provided for molecular markers for molecular breeding, phyletic evolution and germplasm resource identification.
Owner:TIANJIN RES INST OF VEGETABLE
Method for processing iridium alloy bar or plate
ActiveCN102205486ASimple methodEasy to implementArc welding apparatusMetal rolling arrangementsIridiumHydrogen
The invention discloses a method for processing an iridium alloy bar or plate. The method comprises the following steps of: wrapping and welding an iridium alloy to be processed by using a hot-rolled metal molybdenum plate as a wrapping material at first; placing the iridium alloy wrapped by the hot-rolled metal molybdenum plate into a hydrogen resistance furnace and heating; processing the heated iridium alloy wrapped by the hot-rolled metal molybdenum plate to obtain a bar or plate; annealing the obtained bar or plate; and finally immersing the annealed bar or plate into chloroazotic acid and eliminating the wrapped hot-rolled metal molybdenum plate so as to obtain the iridium alloy bar or plate. The method is simple and easy to implement, and no special equipment is needed; and by using the advantage of the metal molybdenum and adopting a wrapping technology, the iridium alloy is wrapped into the hot-rolled metal molybdenum plate, so the iridium alloy cannot be influenced by the environmental condition during processing, and the iridium alloy can be processed successfully.
Owner:NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
Paddy rice male fertility regulating gene OsSTRL2 and application thereof
InactiveCN106834305AControl reproductive processImprove fertilityPlant peptidesFermentationBiotechnologyNucleotide sequencing
The invention discloses a paddy rice male fertility regulating gene OsSTRL2 and application thereof and belongs to the technical field of plant biology. The nucleotide sequence of the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID NO. 1, and the amino acid sequence of protein encoded by the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID No. 2. The invention further provides a method for culturing male sterile plants. The paddy rice male fertility regulating gene OsSTRL2 and a male sterile line generated on the basis of the gene have the advantages that the male sterile line is stable in fertility, unaffected by environment conditions and capable of being recovered by wild transgenosis; the gene and the male sterile line provide necessary components for the building of novel cross breeding systems and are significant in production practice.
Owner:SICHUAN AGRI UNIV
Rice fertility regulating gene and its mutant and application thereof
ActiveCN110511945AStable sterility traitsUnaffected by environmental conditionsVectorsHydrolasesAgricultural sciencePollen
The invention provides a rice fertility regulating gene and its mutant and an application thereof. The invention provides a rice gene GMS1, which has the functions of regulating rice male germ cell development and pollen fertility, a CDS sequence is shown as SEQ ID NO: 2, and an amino acid sequence is shown as SEQ ID NO: 3. The invention provides a radiation mutagenesis mutant and a CRISPR knockout mutant of the GMS1 gene, and provides a molecular marker identification method for the mutant. The rice gene GMS1 provided by the present invention can be used for sterile breeding and production ofrice hybrids, and has great application value and economic value.
Owner:HAINAN BOLIAN RICE GENE TECH CO LTD
Molecular marker authenticating method for related gene of cucumber fruit length
InactiveCN102586405AEasy to operateLow costMicrobiological testing/measurementBiotechnology3-deoxyribose
The invention relates to a molecular marker authenticating method for the related gene of cucumber fruit length, which comprises the following steps of: taking the deoxyribose nucleic acid (DNA) of cucumber variety or strain with unknown fruit length as a template, taking CSWCT28 as a primer to carry out polymerase chain reaction (PCR) amplification, and carrying out 3% agarose gel electrophoresis detection on an amplified product or carrying out 7.5% denaturing polyacrylamide gel electrophoresis detection on the amplified product, wherein if the amplified banding pattern is consistent with the banding pattern of cucumber Dongnong 803 with known short fruit length, the cucumber variety or strain with the unknown fruit length is the variety or strain with short fruit length , and the PCR amplification has the procedures: previously denaturating for 5min under 94 DEG C, denaturating for 30s under 94 DEG C, annealing for 1min under 47 DEG C, carrying out 35 cycles, extending for 1min under 72 DEG C, extending for 10min under 72 DEG C, and finally storing under 4 DEG C. The method is used for authenticating the related gene of the cucumber fruit length.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Method using sequence characterize amplified region (SCAR) signs to identify sugarcane brown rust disease resistance
InactiveCN102586460AReduce dosageHigh sensitivityMicrobiological testing/measurementBiotechnologyGermplasm
The invention relates to a method using sequence characterize amplified region (SCAR) signs to identify sugarcane brown rust disease resistance, which comprises extraction of genome deoxyribonucleic acid (DNA), detection system establishment of SCAR- polymerase chain reaction (PCR) signs and detection of SCAR-PCR products. The method using SCAR signs to identify sugarcane brown rust disease resistance has the advantages of being high in flexibility and small in using amount of DNA. Compared with general resistance identification, the method has the advantages of being convenient in operation,short in detection time, high in accuracy, easy in judgment, good in repeatability, free of effect of environmental conditions, and the like. The method provides a convenient and effective brown rustdisease resistance identification system for assessment of sugarcane germplasm resources and accreditation of new products and has significance on enhancing brown rust resistance sugarcane breading and protection on brown rust resistance sugarcane germplasm resources.
Owner:FUJIAN AGRI & FORESTRY UNIV
SSR (Simple Sequence Repeat) molecular marking method for identifying variety authenticity and/ or variety purity of high-quality transgenic hybrid cotton CCRI (Chinese cotton research institute) 70
ActiveCN102586426AGood repeatabilityHigh polymorphismMicrobiological testing/measurementBiotechnologyGenomic DNA
The invention discloses an SSR (Simple Sequence Repeat) molecular marking method for identifying variety authenticity and / or variety purity of high-quality transgenic hybrid cotton CCRI (Chinese cotton research institute) 70, which comprises the following steps: extracting genomic DNA (Deoxyribose Nucleic Acid) from a cotton hybrid 'CCRI 70' and the patient seed or the young seedling leaves thereof; taking the genomic DNA extracted in step one as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by an SSR primer; carrying out gel electrophoresis on an amplified product; and analyzing an electrophoresis result, wherein a seed or a signal plant is identified as a real hybrid if the seed or the signal plant has special bands of parents, and is identified as a false hybrid if any one band lacks. The SSR molecular marking method has the advantages of good repeatability, high polymorphism, stable hereditary, stable and reliable detection result and low cost, is free from the influence of environmental condition, is quick and accurate, is simple and convenient to operate and is favorable for quickly detecting on a large scale.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Primer sequence and detection method for detecting purity of Jin superior NO.36 cucumber hybridized seeds
InactiveCN102168134ALow costUnaffected by environmental conditionsMicrobiological testing/measurementDNA/RNA fragmentationForward primerAgricultural science
The invention relates to primer sequence and detection method for detecting purity of Jin superior NO.36 hybridized cucumber seeds, wherein the primer sequence consists of a forward primer and a reverse primer, the forward primer is a nucleotide sequence shown in SEQ ID NO.1 in a sequence table, and the reverse primer is a nucleotide sequence shown in SEQ ID NO.2 in the sequence table. The primer sequence and detection method provided by the invention have the advantages of being fast, simple, convenient, stable and reliable, having low cost and being not affected by environment conditions, and the like, can be used for carrying out complete identification of one batch only for 5-6 hours on the purity of the Jin superior NO.36 hybridized cucumber seeds, thus saving a great deal of manpower and land capacity, being fast and accurate in identification result, and having great application value in the purity identification of the cucumber seeds.
Owner:TIANJIN RES INST OF VEGETABLE
Molecular marker identification method for bacterial angular leaf spot resistant gene of cucumber
InactiveCN102102128AReduce the waste of manpower and material resourcesImprove breeding efficiencyMicrobiological testing/measurementPolymerase chain reactionPolyacrylamide
The invention relates to a molecular marker identification method for the bacterial angular leaf spot resistant gene of cucumbers, which comprises: performing polymerase chain reaction (PCR) amplification by using the DNA of a cucumber variety or strain of which the bacterial angular leaf spot resistance is unknown as a template and chimpanzee sanctuary and wildlife conservation trust (CSWCT)24A as a primer; detecting the product of the amplification by electrophoresis in 3-percent gelose gel or 7.5-percent denaturant polyacrylamide gel; and if the band type obtained by amplification is consistent with the band of a known bacterial angular leaf spot resistant cucumber Dongnong 803, determining the cucumber variety or strain of which the bacterial angular leaf spot resistance is unknown as a bacterial angular leaf spot resistant variety or strain. The PCR amplification process comprises: pre-denaturing at 94 DEG C for 5 minutes, denaturing at 94 DEG C for 30 seconds, annealing at 57 DEG C for 1 minute, circulating for 35 times, extending at 72 DEG C for 1 minute, extending at 72 DEG C for 10 minutes, and storing at 4 DEG C. The method is used for identifying the bacterial angular leaf spot resistant gene of cucumbers.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Molecular marker identification method of cucumber branchiness related gene
InactiveCN102533946AOvercoming disadvantages of vulnerability to environmental influencesEasy to operateMicrobiological testing/measurementRelated geneAgarose gel electrophoresis
The invention relates to a molecular marker identification method of cucumber branchiness related gene. The method comprises the steps of: carrying out PCR (polymerase chain reaction) amplification with DNA (deoxyribonucleic acid) of a cucumber variety or line with unknown branchiness and with CSWCT17 as a primer, carrying out 3% agarose gel electrophoresis detection on the amplification product or carrying out 7.5% denaturalized polyacrylamide gel electrophoresis detection on the amplification product, and judging the cucumber variety or line with unknown branchiness to be a variety or line with weak branchiness if the amplified band form is consistent with the band form of cucumber Dongnong 803 with weak branchiness, wherein the PCR amplification process comprises the steps of: pre-denaturizing at 94 DEG C for 5 minutes, denaturalizing at 94 DEG C for 30 seconds, annealing at 67 DEG C for 1 minute, circulating 35 times, drawing at 72 DEG C for 1 minute, drawing at 72 DEG C for 10 minutes, and finally storing at 4 DEG C. The molecular marker identification method provided by the invention is used for identifying the cucumber branchiness related gene.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Epoxy resin sports floor material
InactiveCN108285614AImprove stabilityUnaffected by environmental conditionsConjugated diene hydrocarbon coatingsCarboxyl rubber coatingsFiberEpoxy
The invention belongs to the field of building materials, and provides an epoxy resin sports floor material. The epoxy resin sports floor material is prepared from the following raw materials in partsby weight: 30 to 40 parts of epoxy resin, 10 to 15 parts of butadiene styrene rubber, 1 to 5 parts of carboxyl-terminated liquid nitrile butadiene rubber, 5 to 10 parts of shale fibers, 5 to 10 partsof aluminum sulfate, 1 to 5 parts of butanol, 1 to 5 parts of petroleum ether, 1 to 5 parts of vinyl distearamide and 1 to 5 parts of sodium gluconate. A prepared product is high in stability and cannot be affected by environmental conditions; the shortcoming that a conventional coating epoxy floor product is sensitive to objective environmental conditions such as temperature and humidity in a curing process is overcome; the product is uniform in texture and proper in elasticity.
Owner:钟建军
Primer sequence for judging cucumber powdery mildew resistance and judging method therefor
InactiveCN1215078CUnaffected by environmental conditionsSugar derivativesMicrobiological testing/measurementAgricultural scienceGel electrophoresis
The present invention discloses one kind of primer series for identifying cucumber powdery mildew and its identification method. The primer series consists of upstream primer 5'-CAG TAA ATG AAA GAA AAG AAG-3' and downstream primer 5'-ATA CAT AGC CAT ACA AAA AT-3'. The identification method of cucumber powdery mildew with the primer series includes the following steps: extracting cucumber genome DNA; PCR proliferation; gel electrophoresis analysis of the PCR proliferation product; and identifying the resistance of cucumber powdery mildew according to the relative position of the identified sample on gel strip. The said method has its result possess coincidence rate with field detection result up to 94 %, and is fast, accurate and not affected by environment condition, so that it has high application value in screening cucumber powdery mildew resistance.
Owner:TIANJIN RES INST OF VEGETABLE +1
Method for using sequence characterize amplified region (SCAR) signs to identify sugarcane brown rust disease resistance
InactiveCN102586460BReduce dosageHigh sensitivityMicrobiological testing/measurementBiotechnologyGermplasm
The invention relates to a method using sequence characterize amplified region (SCAR) signs to identify sugarcane brown rust disease resistance, which comprises extraction of genome deoxyribonucleic acid (DNA), detection system establishment of SCAR- polymerase chain reaction (PCR) signs and detection of SCAR-PCR products. The method using SCAR signs to identify sugarcane brown rust disease resistance has the advantages of being high in flexibility and small in using amount of DNA. Compared with general resistance identification, the method has the advantages of being convenient in operation,short in detection time, high in accuracy, easy in judgment, good in repeatability, free of effect of environmental conditions, and the like. The method provides a convenient and effective brown rustdisease resistance identification system for assessment of sugarcane germplasm resources and accreditation of new products and has significance on enhancing brown rust resistance sugarcane breading and protection on brown rust resistance sugarcane germplasm resources.
Owner:FUJIAN AGRI & FORESTRY UNIV
Primer sequence for identifying cucumber ampho disease and its identification method
InactiveCN1307304CUnaffected by environmental conditionsMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceFrost
The invention is a primer sequence for identifying resistance to cucumber frost mildew and identifying method, where the primer sequence is composed of an upper primer with a nucleotide sequence described in sequence list SEQ ID No.1 and an lower primer with that in sequence list SEQ ID No.2; includes the steps: (1) extracting a cucumber gene group DNA; (2) making PCR amplification by using the above two nucleotide sequences; (3) making gel electrophoretic analysis on the product of PCR amplification; (4) according to the relative position of all the identified samples, identifying the resistance to cucumber frost mildew, and the fitting rate of the identified result to the field resistance to diseases reaches 94.7% and it has the advantages of rapidness, accuracy, no influence by the environmental conditions, etc, and has very great application value in screening the resistance to cucumber frost mildew.
Owner:TIANJIN RES INST OF VEGETABLE +1
A kind of rice fertility gene saw1 and its application
ActiveCN110724694BImprove fertilityUnaffected by environmental conditionsBacteriaMicroorganism based processesAnimal scienceHybrid seed
Owner:SOUTH CHINA AGRI UNIV
Gene segments linked with yellow and green cotyledons of cucumbers and application
InactiveCN102242130BUnaffected by environmental conditionsMicrobiological testing/measurementFermentationAgricultural scienceGermplasm
The invention discloses gene segments linked with yellow and green cotyledons of cucumbers and application. The gene segment linked with yellow cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.1, and the gene segment linked with green cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.2. The gene segment linked with yellow cotyledons of cucumbers and the gene segment linked with green cotyledons of cucumbers are used for detecting the constructed F2-generation segregation population and cucumber germplasm resources, the identification shows that the coincidence rate of the detection result and the artificial phenotype reaches 96.77%, and the linkage inheritance distance of the genes related to the colors of cucumber cotyledons is 3.2cm. The invention has the advantages of rapidness, accuracy, no influence of environmental conditions and the like, and has a great application value for molecular marker-assisted breeding of cucumbers.
Owner:TIANJIN RES INST OF VEGETABLE
A kind of rice male fertility regulation gene osstrl2 and its application
InactiveCN106834305BControl reproductive processImprove fertilityPlant peptidesFermentationBiotechnologyNucleotide
The invention discloses a paddy rice male fertility regulating gene OsSTRL2 and application thereof and belongs to the technical field of plant biology. The nucleotide sequence of the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID NO. 1, and the amino acid sequence of protein encoded by the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID No. 2. The invention further provides a method for culturing male sterile plants. The paddy rice male fertility regulating gene OsSTRL2 and a male sterile line generated on the basis of the gene have the advantages that the male sterile line is stable in fertility, unaffected by environment conditions and capable of being recovered by wild transgenosis; the gene and the male sterile line provide necessary components for the building of novel cross breeding systems and are significant in production practice.
Owner:SICHUAN AGRI UNIV
Steel plate rust removal equipment and steel plate rust removal method
PendingCN111941225AEasy to makeEasy to assemblePortable grinding machinesGrinding/polishing safety devicesGear wheelElectric machine
The invention provides steel plate rust removal equipment. The steel plate rust removal equipment comprises a grinding and cleaning head, wherein the grinding and cleaning head is a disc, a replaceable steel wire brush is arranged on the disc, and the disc is connected with a driving motor; the grinding and cleaning head is connected with a long handle, a cable is connected to the rear end of thehandle, and a control switch is arranged on the handle; and a blowing nozzle is arranged in the middle of the grinding and cleaning head, fan blades are arranged inside the blowing nozzle, and the fanblades are connected with a motor shaft of the driving motor through a gear. The steel plate dust removal method comprises the steps that during rust removal, firstly, a rust remover is uniformly sprayed on a steel plate needing rust removal; then rust removing and grinding are carried out on the steel plate by using the rust removal equipment with the steel wire brush; and finally, antirust paint is sprayed on the steel plate.
Owner:SHANGHAI STRONG FOUND ENG
Primer sequence for identifying resistance of cucumber against alternaria cucumerina and identification method thereof
InactiveCN101845504BUnaffected by environmental conditionsMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The invention discloses a primer sequence for identifying resistance of cucumber against alternaria cucumerina and an identification method thereof. The primer sequence comprises nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2 in a sequence table. The method for identifying the resistance of the cucumber against the alternaria cucumerina comprises the following steps of: (1) extracting genomic DNA of the cucumber; (2) performing polymerase chain reaction (PCR) amplification by taking the nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2 in the sequence table as primers; (3) performing gel electrophoresis analysis on the amplification product; and (4) identifying the resistance of the cucumber against the alternaria cucumerina according to the relative position of each identified sample on a gel strip. By evaluating the resistance of the constructed segregation population of generation F2 and cucumber germplasm resources against the alternaria cucumerina with the primer sequence of the invention, the coincidence rates for the result and artificial inoculation evaluation reach 95.04 percent and 94.74 percent respectively; the linkage genetic distance with the alternaria cucumerina resistance gene is 4.96cm; and the primer sequence has the advantages of high speed, accuracy and freedom from environmental condition and the like, and has great application value for screening the resistance of the resources in the cucumber breeding.
Owner:TIANJIN RES INST OF VEGETABLE
Nucleotide sequence and detection method for detecting purity of Jinyou #401 cucumber hybrid seed
InactiveCN104762394ALow costUnaffected by environmental conditionsMicrobiological testing/measurementDNA/RNA fragmentationAmplification dnaCucumber seed
The invention discloses a nucleotide sequence for detecting the purity of Jinyou #401 cucumber hybrid seeds. The nucleotide sequence has nucleotide as shown in SEQ ID NO:1-2. A method for identifying the purity of the cucumber seeds by using the nucleotide sequence comprises the following steps: (1) extracting DNA of a tested material; (2) performing PCR amplification by using the nucleotide sequence as shown in SEQ ID No.1 and the nucleotide sequence as shown in SEQ ID No.2 in the sequence table; (3) performing gel electrophoresis analysis on amplified DNA fragments; and (4) identifying the purity of the cucumber seeds according to the conditions of strips of detected samples on gel. The nucleotide sequence has the advantages of rapidness, simplicity and convenience, stability, reliability, low cost, no environment condition influence and the like, and has great application values in cucumber seed purity identification.
Owner:TIANJIN RES INST OF VEGETABLE
Method for processing iridium alloy bar or plate
ActiveCN102205486BSimple methodEasy to implementArc welding apparatusMetal rolling arrangementsIridiumHydrogen
The invention discloses a method for processing an iridium alloy bar or plate. The method comprises the following steps of: wrapping and welding an iridium alloy to be processed by using a hot-rolled metal molybdenum plate as a wrapping material at first; placing the iridium alloy wrapped by the hot-rolled metal molybdenum plate into a hydrogen resistance furnace and heating; processing the heated iridium alloy wrapped by the hot-rolled metal molybdenum plate to obtain a bar or plate; annealing the obtained bar or plate; and finally immersing the annealed bar or plate into chloroazotic acid and eliminating the wrapped hot-rolled metal molybdenum plate so as to obtain the iridium alloy bar or plate. The method is simple and easy to implement, and no special equipment is needed; and by using the advantage of the metal molybdenum and adopting a wrapping technology, the iridium alloy is wrapped into the hot-rolled metal molybdenum plate, so the iridium alloy cannot be influenced by the environmental condition during processing, and the iridium alloy can be processed successfully.
Owner:NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
An optical soliton generation device and method with adjustable center frequency
InactiveCN104375353BSolve the problem of adjustable frequencySimple methodNon-linear opticsMicro nanoTransfer procedure
Owner:SHANTOU UNIV
A kind of rice fertility regulation gene and its mutant and application
ActiveCN110511945BStable sterility traitsUnaffected by environmental conditionsVectorsHydrolasesBiotechnologyMutagenesis
The invention provides a rice fertility regulation gene, its mutant and application. The invention provides a rice gene GMS1 with functions of regulating rice male germ cell development and pollen fertility, its CDS sequence is shown in SEQ ID NO:2, and its amino acid sequence is shown in SEQ ID NO:3. The invention provides radiation mutagenesis mutants and CRISPR knockout mutants of the GMS1 gene, and a molecular marker identification method for the mutants. The rice gene GMS1 provided by the invention can be used for sterile seed production and production of rice hybrids, and has great application value and economic value.
Owner:HAINAN BOLIAN RICE GENE TECH CO LTD
DNA molecular identification method of Haliotis sieboldii and Haliotis discus Hannai hybrid
InactiveCN101353702BHigh polymorphismGenetic stabilityMicrobiological testing/measurementMolecular identificationMicrosatellite
The invention discloses a DNA molecule identification method of coenospecies of Xishi abalone and Haliotis discus hannai Ino, relating to the DNA molecule marker detection of an abalone, in particular to a method for carrying out molecule identification to the authenticity of the coenospecies of an interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino by utilizing a molecule marker technology (microsatellite technology). The invention provides a DNA molecule identification method of the coenospecies of the Xishi abalone and the Haliotis discus hannai Ino by using microsatellite molecular marker, which has the advantages of high polymorphism, stable heredity and not being affected by environment conditions, etc, and can identify the authenticity of the coenospecies of the interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino during the large scale interspecific hybridization species production process of the Xishi abalone and the Haliotis discus hannai Ino. The genome DNA of the coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino is extracted by adopting a phenol chloroform method and PCR amplification reaction is carried out by taking the extracted genome DNA as a template to obtain the amplification product; gel electrophoresis and dyeing are carried out to the amplification product, and the map is compared; and comparison is carried out according to the electrophoresis result and the provided standard map.
Owner:XIAMEN UNIV
Molecular marker authenticating method for related gene of cucumber propamocarb residue
InactiveCN102586403AEasy to operateLow costMicrobiological testing/measurementBiotechnology3-deoxyribose
The invention relates to a molecular marker authenticating method for the related gene of cucumber propamocarb residue. The method of the invention comprises the following steps of: taking the deoxyribose nucleic acid (DNA) of cucumber variety or strain with unknown propamocarb residue as a template, taking CSWCT14 as a primer to carry out polymerase chain reaction (PCR) amplification, and carrying out 3% agarose gel electrophoresis detection on an amplified product or carrying out 7.5% denaturing polyacrylamide gel electrophoresis detection on the amplified product, wherein if the amplified banding pattern is consistent with the banding pattern of cucumber Dongnong 803 with known low-propamocarb residue, the cucumber variety or strain with the unknown propamocarb residue is the variety or strain with low propamocarb residue, and the PCR amplification has the procedures: previously denaturating for 5min under 94 DEG C, denaturating for 30s under 94 DEG C, annealing for 1min under 57 DEG C, carrying out 35 cycles, extending for 1min under 72 DEG C, extending for 10min under 72 DEG C, and finally storing under 4 DEG C. The method is used for authenticating the related gene of the cucumber propamocarb residue.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
A marker gene for identifying heat-tolerant blueberry varieties and its application
ActiveCN104830979BStable expressionEasy to operateMicrobiological testing/measurementFermentationAgricultural scienceTotal rna
The invention relates to a marker gene for identifying blueberry heat-resistant varieties and application thereof, belonging to the field of plant biotechnology. Utilizing the marker gene, thioredoxin o-type (TRXo), was able to up-regulate expression to different degrees in different heat-tolerant blueberry varieties under high temperature stress. Primers were designed using the CDS sequence of the gene as a template, and the detection method was to artificially simulate the climate and heat stress at 45°C for 6 h, extract total RNA, reverse transcribe it into cDNA as a template, perform qRT‑PCR detection, and use the 2‑ΔΔCt method to calculate analyze. By analyzing the ratio of the expression of the gene under heat stress and the control, if the ratio is greater than or equal to 5, the blueberry variety can be identified as a heat-tolerant variety. Compared with the field natural identification method, the method is faster, more stable and more accurate, and is the basis for rapid screening of heat-resistant varieties for popularization and planting and breeding of new heat-resistant varieties.
Owner:ZHEJIANG NORMAL UNIVERSITY
Primer sequence for detecting purity of hybrid seeds of Jinyou No.35 cucumbers and detection method
InactiveCN102168135ALow costUnaffected by environmental conditionsMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceHybrid seed
The invention relates to a primer sequence for detecting the purity of hybrid seeds of Jinyou No.35 cucumbers and a detection method. The primer sequence consists of an upstream primer and a downstream primer, wherein the upstream primer is a nucleotide sequence shown in SEQ ID NO.1 in a sequence table; and the downstream primer is a nucleotide sequence shown in SEQ ID NO.2 in the sequence table. The detection method has the characteristics of quickness, simpleness and convenience, stability, reliability, low cost and the like, and is not affected by environmental conditions. The identification on the purity of one batch of hybrid seeds of the Jinyou No.35 cucumbers can be finished within 5 to 6 hours. A large amount of manpower and soil fertility is saved, and identification results are quick and accurate. The primer sequence and the detection method have high application value on the identification of the purity of cucumber seeds.
Owner:TIANJIN RES INST OF VEGETABLE
Rice fertility regulation gene GMS3 as well as mutant and application thereof
PendingCN114540366AStable sterility traitsUnaffected by environmental conditionsPlant peptidesFermentationBiotechnologyMutagenesis
The invention provides a rice fertility regulation gene GMS3 as well as a mutant and application thereof. The invention provides a rice gene GMS3 with functions of regulating and controlling rice male germ cell development and pollen fertility, the CDS sequence of the rice gene GMS3 is as shown in SEQ ID NO: 2, and the amino acid sequence of the rice gene GMS3 is as shown in SEQ ID NO: 3. The invention provides a radiation mutagenesis mutant of a GMS3 gene and a CRISPR (clustered regularly interspaced short palindromic repeats) knockout mutant, and provides a molecular marker identification method of the mutants. The rice gene GMS3 provided by the invention can be used for sterile seed production and production of rice hybrids, and has huge application value and economic value.
Owner:HAINAN BOLIAN RICE GENE TECH CO LTD
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