34results about How to "Unaffected by environmental conditions" patented technology

The invention is a primer sequence for identifying resistance to cucumber frost mildew and identifying method, where the primer sequence is composed of an upper primer with a nucleotide sequence described in sequence list SEQ ID No.1 and an lower primer with that in sequence list SEQ ID No.2; includes the steps: (1) extracting a cucumber gene group DNA; (2) making PCR amplification by using the above two nucleotide sequences; (3) making gel electrophoretic analysis on the product of PCR amplification; (4) according to the relative position of all the identified samples, identifying the resistance to cucumber frost mildew, and the fitting rate of the identified result to the field resistance to diseases reaches 94.7% and it has the advantages of rapidness, accuracy, no influence by the environmental conditions, etc, and has very great application value in screening the resistance to cucumber frost mildew.

The invention discloses a rice fertility gene SAW1 and its application. The nucleotide sequence of the gene SAW1 is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. Knocking out the gene SAW1 can abort rice anthers, and can be applied to the creation of sterile lines and the preparation of hybrid seeds. The male sterile line produced based on the mutation of gene SAW1 has stable fertility and is not affected by environmental conditions. It provides necessary elements for the construction of a new hybrid breeding system and has good application value and prospects.

The invention discloses gene segments linked with yellow and green cotyledons of cucumbers and application. The gene segment linked with yellow cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.1, and the gene segment linked with green cotyledons of cucumbers is a nucleotide sequence shown in SEQ ID No.2. The gene segment linked with yellow cotyledons of cucumbers and the gene segment linked with green cotyledons of cucumbers are used for detecting the constructed F2-generation segregation population and cucumber germplasm resources, the identification shows that the coincidence rate of the detection result and the artificial phenotype reaches 96.77%, and the linkage inheritance distance of the genes related to the colors of cucumber cotyledons is 3.2cm. The invention has the advantages of rapidness, accuracy, no influence of environmental conditions and the like, and has a great application value for molecular marker-assisted breeding of cucumbers.

The invention discloses a paddy rice male fertility regulating gene OsSTRL2 and application thereof and belongs to the technical field of plant biology. The nucleotide sequence of the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID NO. 1, and the amino acid sequence of protein encoded by the paddy rice male fertility regulating gene OsSTRL2 is as shown in SEQ ID No. 2. The invention further provides a method for culturing male sterile plants. The paddy rice male fertility regulating gene OsSTRL2 and a male sterile line generated on the basis of the gene have the advantages that the male sterile line is stable in fertility, unaffected by environment conditions and capable of being recovered by wild transgenosis; the gene and the male sterile line provide necessary components for the building of novel cross breeding systems and are significant in production practice.

The invention relates to the field of interaction of laser and matter in strong-field physics, in particular to an optical soliton generation device and method with an adjustable center frequency. The optical soliton generation device comprises a laser, an action structure and a receiver. The method includes the steps that S1, an interval d between periodical medium planes is selected, the size delta of a periodical medium is obtained according to the formula that d=2*delta, and a periodical micro-nano structure is formed; S2, short-period ultra-short laser pulse is introduced, and the laser pulse is transmitted in the medium; S3, the reflection electric field and the transmission electric field generated when the pulse is transmitted in the periodical micro-nano structure are recorded respectively; S4, the recorded data are processed and analyzed, and then soliton pulse is obtained. By changing the size of the medium in the periodical micro-nano structure, the transmission process of the pulse in the micro-nano structure is affected, the final transmission electric field is further affected, and solitons of different frequencies are obtained. The optical soliton generation method is simple, easy to implement, free of influences of environment conditions and good in stability and repeatability of the system, and well realizes the adjustable center frequency of the optical solitons.

The invention discloses a DNA molecule identification method of coenospecies of Xishi abalone and Haliotis discus hannai Ino, relating to the DNA molecule marker detection of an abalone, in particular to a method for carrying out molecule identification to the authenticity of the coenospecies of an interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino by utilizing a molecule marker technology (microsatellite technology). The invention provides a DNA molecule identification method of the coenospecies of the Xishi abalone and the Haliotis discus hannai Ino by using microsatellite molecular marker, which has the advantages of high polymorphism, stable heredity and not being affected by environment conditions, etc, and can identify the authenticity of the coenospecies of the interspecific coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino during the large scale interspecific hybridization species production process of the Xishi abalone and the Haliotis discus hannai Ino. The genome DNA of the coenospecies generation of the Xishi abalone and the Haliotis discus hannai Ino is extracted by adopting a phenol chloroform method and PCR amplification reaction is carried out by taking the extracted genome DNA as a template to obtain the amplification product; gel electrophoresis and dyeing are carried out to the amplification product, and the map is compared; and comparison is carried out according to the electrophoresis result and the provided standard map.

The invention relates to a marker gene for identifying blueberry heat-resistant varieties and application thereof, belonging to the field of plant biotechnology. Utilizing the marker gene, thioredoxin o-type (TRXo), was able to up-regulate expression to different degrees in different heat-tolerant blueberry varieties under high temperature stress. Primers were designed using the CDS sequence of the gene as a template, and the detection method was to artificially simulate the climate and heat stress at 45°C for 6 h, extract total RNA, reverse transcribe it into cDNA as a template, perform qRT‑PCR detection, and use the 2‑ΔΔCt method to calculate analyze. By analyzing the ratio of the expression of the gene under heat stress and the control, if the ratio is greater than or equal to 5, the blueberry variety can be identified as a heat-tolerant variety. Compared with the field natural identification method, the method is faster, more stable and more accurate, and is the basis for rapid screening of heat-resistant varieties for popularization and planting and breeding of new heat-resistant varieties.