Nucleotide sequence and detection method for detecting purity of Jinyou #401 cucumber hybrid seed

Abstract

The invention discloses a nucleotide sequence for detecting the purity of Jinyou #401 cucumber hybrid seeds. The nucleotide sequence has nucleotide as shown in SEQ ID NO:1-2. A method for identifying the purity of the cucumber seeds by using the nucleotide sequence comprises the following steps: (1) extracting DNA of a tested material; (2) performing PCR amplification by using the nucleotide sequence as shown in SEQ ID No.1 and the nucleotide sequence as shown in SEQ ID No.2 in the sequence table; (3) performing gel electrophoresis analysis on amplified DNA fragments; and (4) identifying the purity of the cucumber seeds according to the conditions of strips of detected samples on gel. The nucleotide sequence has the advantages of rapidness, simplicity and convenience, stability, reliability, low cost, no environment condition influence and the like, and has great application values in cucumber seed purity identification.

Description

technical field [0001] The invention relates to a DNA sequence in biotechnology and a method for identifying the purity of Jinyou 401 cucumber hybrid seeds using the sequence. Background technique [0002] Seed purity identification is a key link to ensure seed quality. With the acceleration of the breeding process, the number of new cucumber varieties is increasing, and the amount of materials that need to be identified for variety purity will increase. In the past, the conventional purity identification was carried out in the field. During the fruiting period, experts went to the field to observe the characteristics and consistency of the varieties. There was a long cycle, a heavy workload, and it was easily affected by environmental factors, and the phenotypic characteristics would have a certain degree of deviation. Problems such as these may affect the accuracy of variety purity identification, and directly affect the promotion of improved varieties; in addition, due t...

AI Technical Summary

Problems solved by technology

In the past, the conventional purity identification was carried out in the field. During the fruiting period, experts went to the field to observe the characteristics and consistency of the varieties. There was a long cycle, a heavy workload, and it was easily affected by environmental factors, and the phenotypic characteristics would have a certain degree of deviation. Such problems may affect the accuracy of variety purity identification and directly affect the promotion of improved varieties; in addition, due to the long identification period, the seeds produced in the current year are often not sold until the next year, resulting in loss of business opportunities

[0003] Jinyou 401 cucumber hybrid seeds are the first-generation hybrid seeds obtained by pollination of the male parent and the female parent. It has the characteristics of high yield and excellent traits of the hybrid variety, but at the same time there is a risk of being mixed with the self-bred seeds obtained by the self-fertilization of the female parent. In the past, the identification of variety purity often required field cultivation, observing plants and commercial melons to determine whether it was a hybrid first-generation variety. There are two main disadvantages: the first is the long cycle, and the general time for receiving seeds is from July to October every year. , Jinyou 401 is an open-field variety that needs to be sown in spring or early summer for identification, so it often needs to wait until the next year for cultivation identification, and each time it takes at least 50 days from cultivation to commercial melon emergence, resulting in the newly harvested seeds not being on the market in time; The second identification standard is vague, and the identification standard of field identification is very subjective. It is completely compared with the plants and commercial melons that have been confirmed to be hybrid first-generation seeds through visual inspection. The standard is not clear.

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