Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

31results about How to "Realize trace detection" patented technology

Magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and preparation method

InactiveCN108020666ARealize quantitative detection of single servingHigh precisionMaterial analysisMedicineQuality control
The invention discloses a magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and a preparation method. The test strip is prepared by successively binding a sample pad, a combining pad, a nitrocellulose membrane and water absorbing paper to a bottom plate of the test strip and 2mm of adjacent parts are alternately overlaid one another, wherein the nitrocellulose membrane is pre-coated with a detection line of a CEA antibody and a CA19-9 antibody and a quality control line of a rabbit antimouse IgG antibody. Compared with existingtumor marker detection technologies, the magnetic immunochromatographic technology is creatively introduced into combined quantitative detection of CEA and CA19-9 to achieve microscale quantitative analysis of CEA and CA19-9, so that the detecting sensitivity is improved greatly; and based on double positive CEA and CA19-9, the occurrence rate and transfer rate of tumor can be further judged to improve early stage diagnostic rate of occurrence and transfer of colon cancer to achieve the purpose of immediate detection and quick diagnosis, and the test strip is simple to operate and low in cost, and can be widely popularized.
Owner:FUZHOU UNIV

Delta type resonant cavity/integrating sphere combination reinforcing cavity capable of improving sensitivity of gas Raman detection

The invention relates to a delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of gas Raman detection. The delta type resonant cavity / integrating sphere combination reinforcing cavity comprises three endoscopes (M1, M2 and M3) with high reflectivity, a gold-plated integrating sphere, inverse reflecting mirror (M4) with high reflectivityand a sealing chamber, wherein the three endoscopes (M1, M2 and M3) with the high reflectivity are inlaid in three holes corresponding to the gold-plated integrating sphere, the inverse reflecting mirror (M4) with the high reflectivity is arranged outside the gold-plated integrating sphere and in rear of the endoscope (M3), and the inverse reflecting mirror (M4) is arranged in the sealing chamber. The delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of the gas Raman detection has the advantages that for the problems that an existing detection device for characteristic gases in a change-over state is low in detection accuracy and cannot measure and distinguish multiple characteristic gases simultaneously, the technical bottleneck that the minimum gas detection concentration of a Raman spectrum cannot meet the requirements is broken through, and the accuracy and sensitivity of the detection of the characteristic gases in the change-over state are greatly improved.
Owner:CHONGQING UNIV +1

Hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously, and preparation method thereof

The invention relates to a preparation method of hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously. The detection problem of the IL-6, IL-4 and TNF-alpha can be effectively solved. The method comprises the following steps: preparing anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody for hollow gold-silver bimetallic mark and envelope detection lineso as to respectively prepare anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody hollow gold-silver bimetallic markers, drying and curing the hollow gold-silver bimetallic marker, and orderly enveloping the anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibodies for envelope detection line on a nitrocellulose membrane to form the detection line, enveloping the anti-rat immune globulin on the nitrocellulose membrane to form a quality control line of the envelope membrane, thereby obtaining the test strip. The preparation method disclosed by the invention is simple in process, novel and unique, and capable of quickly, simply, early and effectively diagnosing and identifying stroke.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI

Photocatalytic hydrogen production system and hydrogen production method

The invention relates to a photocatalytic hydrogen production system and a gas production measuring method. The photocatalytic hydrogen production system comprises an inert gas source, a photocatalytic reaction device, a gas collecting, discharging and measuring device, a gas detection and verification device and a main valve control pipeline, wherein the main valve control pipeline is connected with the inert gas source, the photocatalytic reaction device, the gas collecting, discharging and measuring device and the gas detection and verification device sequentially, a hydrogen fuel cell is arranged in the gas detection and verification device, and the hydrogen fuel cell is electrically connected with a load. Two ends of the hydrogen fuel cell are also respectively connected with a voltage display gauge. By adopting the photocatalytic hydrogen production system, the integration of the photocatalytic reaction, the gas production measurement and gas detection and verification can be realized; the gas measuring and detection method is simple, visual and effective; the operation difficulty is lowered, and the maintainability is improved; the product cost, the maintenance cost and the running environment cost can be greatly reduced.
Owner:BEIJING PERFECTLIGHT SCI & TECH

Capillary electrophoretic analysis method for trace detection of phenazine-1-carboxylic acid

The invention relates to an analysis method for micro-detecting the capillary electrophoresis of phenazine-1-carboxylic acid, which comprises the quantitative analysis of an internal standard method and an external reference method. Electrophoresis conditions are that: a quartz capillary column with an inner diameter of between 50 and 100mu m is adopted, and the length is between 30 and 70cm; the separation voltage is between 15 and 25kV; the detection wavelength is between 230 and 260nm; the column temperature is between 23 and 28 DEG C; buffer solution is phosphorous acid buffer solution with a concentration of between 5 and 20mM, and the pH value is between 6.0 and 7.0; and a sample loading mode is to load for 60 to 80s at a height pressure difference of between 10 and 20cm, and a sample is dissolved into methanol or methanol solution containing an internal standard substance with certain concentration. The method has good repetitiveness, is simple, convenient and feasible, performs on-line enrichment on the phenazine-1-carboxylic acid by electrostacking enrichment technology during the detection, greatly reduces the limit of detection of the method, and has better linear relation when the phenazine-1-carboxylic acid is between 0.075 and 30mu g / mL.
Owner:SHANGHAI JIAO TONG UNIV

Preparation method of carbon dot and array thereof

The invention discloses a preparation method of a carbon dot and an array thereof. A nano-gold macromolecular membrane is illuminated by laser so that nano-gold is subjected to surface plasmon resonance and temperature rising, and a macromolecular structure at the periphery is damaged to form the carbon dot. The method disclosed by the invention is simple to operate and the preparation success rate can reach 100 percent; the prepared carbon dot can send out intensive fluorescent light under the stimulation of light in a range of 220nm to 550nm; the carbon dots can be used for preparing various arrayed carbon dot arrays; the fluorescence intensity of the carbon dot can be controlled through adjusting the laser illumination time and power and controlling the size of the carbon dot; the prepared carbon dot array can form an invisible fluorescence array and encrypted information transmission can be realized. Meanwhile, trace detection of ions or molecules can also be realized by utilizing quenching characteristics of the ions or molecules to be detected on the fluorescent light.
Owner:GUANGZHOU CLUSTERBIOPHOTON TECH CO LTD

Surface-enhanced Raman scattering substrate and preparation method and application thereof

The invention provides a surface-enhanced Raman scattering substrate and a preparation method and application thereof, and belongs to the technical field of surface-enhanced Raman scattering films, the surface-enhanced Raman scattering substrate comprises a substrate and a three-dimensional noble metal nanostructure; and the substrate is an NAAO film, and the noble metal is Ag. The preparation method comprises the following steps: complexing Fe < 3 + > and tannic acid (TA) on the surface of an NAAO film to obtain NAAO (at) TA / Fe < 3 + >, and further performing in-situ deposition in a silver-containing solution to form the surface-enhanced Raman scattering substrate. A step-by-step assembly method is adopted, a metal polyphenol coordination polymerization network is used for coating an NAAO film, a TA / Fe < 3 + > complex on the surface deposits a large-area uniform silver nanostructure (AgNS) on the NAAO substrate in situ, the surface enhanced Raman scattering substrate is prepared, the surface enhanced Raman scattering substrate has the advantages of uniformity, large area, high sensitivity and the like, and on-site detection of trace fluoroacetic acid can be achieved.
Owner:BEIJING CENT FOR DISEASE PREVENTION & CONTROL

Molecular marker and application thereof in detection of vascular immunoblastic T cell lymphoma

The invention discloses a molecular marker and an application thereof in detection of vascular immunoblastic T cell lymphoma, and belongs to the technical field related to blood disease detection. According to the application, a combination of multiple markers disclosed by the invention is applied to flow cytometry detection of vascular immunoblastic T cell lymphoma and / or monitoring of minimal residual focuses. The molecular marker disclosed by the invention can be widely applied to living cell samples such as bone marrow, peripheral blood, lymph nodes, puncture objects, skin tissues, pleural effusion, peritoneal effusion and the like of a patient, the combination of various markers is effectively arranged, abnormal cells of AITL can be quickly screened out in an early stage, the sensitivity and specificity to tissue samples reach 100%, the use amount of cells is small, and operation is rapid, simple and convenient. Meanwhile, for a patient subjected to chimeric antigen receptor T cell treatment, the composition disclosed by the invention can also be used for evaluating the immunofluorescence intensity of an antigen target spot, the immunofluorescence intensity is an indispensable part in cell treatment, and subsequent residue monitoring is not influenced by treatment.
Owner:TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH

Sulfide detection method

The invention provides a sulfide detection method. The detection method comprises the following steps: (1) mixing a liquid to be detected and a derivatization reagent, and carrying out a reaction to obtain a derivatization product; (2) mixing the derivatization product obtained in the step (1), a Raman surface enhancer and a coagulant, and performing detection in a Raman spectrometer to obtain a Raman spectrogram; and (3) analyzing the peak position and the peak intensity in the Raman spectrogram obtained in the step (2) to obtain a sulfide detection result. In the sulfide detection method, detecting, complex collection, pretreatment and storage processes of the sample to be detected are not needed, large-scale instruments are not needed, rapid, accurate and real-time detection of sulfidescan be realized, the detection limit is lower, the sensitivity is higher, the steps are more convenient, trace detection with the concentration of 0.02 mg / L can be realized, and the method is specially suitable for real-time and on-site detection of the sulfides in drinking water and other water bodies.
Owner:WUXI ZODOLABS BIOTECH

Human EGFR gene mutation site T790M detection reagent and detection method

The invention relates to a human EGFR gene mutation site T790Mdetection reagent and detection method. The detection reagent is prepared from a PCR reaction solution I, a PCR reaction solution II, an enzymatic digestion reaction solution,single base extension liquid I, single base extension liquid II and a magnetic particle suspension. The detection method based on the reagent comprises the steps that the PCR reaction solution I and the PCR reaction solution II amplifyan EGFR genein a blood sample, the enzymatic digestion reaction solutionis added for digestion, and the single base extension liquid I, the single base extension solution II and a PCR-SAP product are added into the digested product for single base extension, and product magnetic beads are gathered and then subjected to chip analysis. According to the detection reagent and method used for detecting human EGFR gene p.T790 mutation, as little as 10 mutant DNAs can be detected from 10<4> DNA molecules, the sensitivity reaches0.1%, and T790M micro-detection is achieved.
Owner:WENZHOU MEDICAL UNIV

Method for detecting hydrazine hydrate

The invention relates to a method for rapidly and repeatedly detecting trace hydrazine hydrate, and belongs to the technical field of inorganic detection. The method for detecting the trace hydrazinehydrate specifically comprises the following steps of adding a to-be-detected substance into a mixed detection solution of a solvent and elemental sulfur, and stirring and uniformly mixing, wherein the to-be-detected substance contains the hydrazine hydrate when the mixed detection solution can display blue, the solvent is N,N-dimethylformamide, N,N-dimethylacetamide and N-methyl pyrrolidone or dimethyl sulfoxide, and the mass fraction of the elemental sulfur in the mixed detection solution is 0.0001%-1%. The method for detecting the trace hydrazine hydrate can be used for repeatedly and rapidly realizing trace detection of the hydrazine hydrate.
Owner:SICHUAN UNIV

Method of establishing double-detection sensor for antibiotics based on aptamers

The invention discloses a method of establishing a double-detection sensor for antibiotics based on aptamers, belonging to the field of detection of substances such as antibiotics. The aptamer sensorcapable of simultaneously detecting two antibiotics, namely chloramphenicol and kanamycin, is established, so experiment cost is reduced as much as possible, and rapid and high-sensitivity detection of antibiotic residues is realized. According to the invention, chloramphenicol and kanamycin aptamers are taken as examples; an auxiliary short chain with one end modified by biotin is used for forming a partially-complementary composite chain structure which is fixed on magnetic beads; after target substance antibiotics are added, the aptamers are separated from the magnetic beads into a solutionthrough high affinity between the antibiotics and the aptamers, the content of the corresponding aptamer is determined by using a real-time fluorescent quantitative PCR method, and an aptamer biological detector for joint detection of the antibiotics is established. By means of the high-specificity affinity effect between the antibiotics and the aptamers, the contents of free aptamers obtained through competition are quantitatively analyzed through the q-PCR method, and therefore, the contents of the two antibiotics are obtained.
Owner:BEIJING UNIV OF CHEM TECH

Fluorescence analysis method for determinating artemisinin content

The invention belongs to the technical field of spectral analysis and detection, and particularly relates to a fluorescence analysis method using a novel nanomaterial, namely graphene quantum dots, inparticular to a fluorescence analysis method for determinating the artemisinin content. A peroxy bridge structure of artemisinin reacts with p-aminophenylboronic acid, a catalytic oxidation reactionis further performed in the presence of oxygen to form p-iminobenzoquinone, and the fluorescence of the graphene quantum dots is quenched. The fluorescence detection method provided by the invention is low in cost, easy to operate, low in detection limit, good in selectivity and high in sensitivity. By the fluorescence analysis method, application of the graphene quantum dots to the field of medicine analysis and detection is expanded, and a convenient and accurate technical means is provided for evaluation and supervision of the quality of an artemisinin medicine.
Owner:湘西自治州奥瑞克医药化工有限责任公司

Automatic nitrite analyzer and detection method thereof

ActiveCN102426148ARealize a wide range of detectionRealize trace detectionMaterial analysisNitrite concentrationNitrogen gas
The present invention discloses a nitrite detection method. The method comprises the following steps that: 1) a nitrite-containing material requiring detection and a reagent are placed in a reaction tank, the nitrite reacts with the reagent to generate nitrogen gas; 2) the pressure of the nitrogen gas is detected; 3) the concentration of the nitrite in the material requiring detection is calculated according to the pressure of the nitrogen gas. According to the present invention, the reagent is adopted to carry out the reaction with the nitrite to generate the N2; after completing the reaction, the pressure value is detected, and the nitrite content is calculated; the reaction of the reagent and the nitrite is performed in a closed reaction tank, and the N2 pressure is detected; according to the gas state equation, the pressure is proportional to the amount of the N2, and the amount of the N2 is proportional to the concentration of the nitrite, such that the nitrite concentration in the water body can be obtained by detecting the N2 pressure. With the present invention, the wide range detection of the nitrite can be achieved, and both the micro detection and the constant detection can be achieved. The present invention further discloses an automatic nitrite analyzer adopting the detection method.
Owner:LIHE TECH (HUNAN) CO LTD

Triangular Resonator/Integrating Sphere Combined Enhancement Cavity for Improving Gas Raman Intensity

The invention relates to a delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of gas Raman detection. The delta type resonant cavity / integrating sphere combination reinforcing cavity comprises three endoscopes (M1, M2 and M3) with high reflectivity, a gold-plated integrating sphere, inverse reflecting mirror (M4) with high reflectivityand a sealing chamber, wherein the three endoscopes (M1, M2 and M3) with the high reflectivity are inlaid in three holes corresponding to the gold-plated integrating sphere, the inverse reflecting mirror (M4) with the high reflectivity is arranged outside the gold-plated integrating sphere and in rear of the endoscope (M3), and the inverse reflecting mirror (M4) is arranged in the sealing chamber. The delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of the gas Raman detection has the advantages that for the problems that an existing detection device for characteristic gases in a change-over state is low in detection accuracy and cannot measure and distinguish multiple characteristic gases simultaneously, the technical bottleneck that the minimum gas detection concentration of a Raman spectrum cannot meet the requirements is broken through, and the accuracy and sensitivity of the detection of the characteristic gases in the change-over state are greatly improved.
Owner:CHONGQING UNIV +1

Triarylamine derivative fluorescent probe as well as preparation method and application thereof

The invention discloses a triarylamine derivative fluorescent probe as well as a preparation method and application thereof, and relates to a triarylamine derivative fluorescent probe containing a thioacetal structure as well as a synthesis method and application of the probe. The preparation process of the novel triarylamine derivative fluorescent probe comprises the following steps: carrying outcoupling reaction on N-(4-biphenyl)-(9,9-dimethylfluoren-2-yl)amine and p-chlorobenzaldehyde, and carrying out aldehyde thioacetal protection reaction on the obtained product. According to the fluorescent probe, in a capronitrile (ACN) / buffer solution (HEPES) (9 / 1, v / v) solution, Hg<2+> is used for promoting the deprotection effect of thioacetal to cause the fluorescence effect change of a detection system, so that the on-off detection of Hg<2+> is realized. The probe has the advantages of short response time, good ion selectivity, strong interference resistance to other metal ions and the like in the Hg<2+> detection process, meanwhile, the detection limit is as low as 1.57*10<-8> mol / L, and trace detection of Hg<2+> can be realized. The method is applied to Chinese herbal medicine Hg<2+> detection.
Owner:HARBIN UNIV OF SCI & TECH

Test strip for simultaneously and quantitatively detecting NSE and S100B in blood and preparation method thereof

The invention belongs to the technical field of medical detection, and relates to a magnetic immunochromatographic test strip for simultaneously and quantitatively detecting NSE and S100B in blood anda preparation method thereof. The test strip is improved, and a magnetic immunochromatographic technology is creatively introduced into the combined detection of the NSE and the S100B for the first time, and by combining a magnetic signal detector, quantitative detection of NSE and the S100B for a single person can be realized simultaneously; and the test strip has the characteristics of being rapid, simple, practical, high in specificity and high in sensitivity, and can be used for remarkably improving the accuracy of cerebral apoplexy detection, that is, on the basis of double positive of NSE and S100B, the cerebral apoplexy occurrence rate can be further judged, so that the early diagnosis rate and prognosis monitoring of cerebral apoplexy can be improved, and the method has relativelyhigh clinical application significance.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI

Automatic nitrite analyzer and detection method thereof

ActiveCN102426148BRealize a wide range of detectionRealize trace detectionMaterial analysisNitrite testNitrogen gas
The present invention discloses a nitrite detection method. The method comprises the following steps that: 1) a nitrite-containing material requiring detection and a reagent are placed in a reaction tank, the nitrite reacts with the reagent to generate nitrogen gas; 2) the pressure of the nitrogen gas is detected; 3) the concentration of the nitrite in the material requiring detection is calculated according to the pressure of the nitrogen gas. According to the present invention, the reagent is adopted to carry out the reaction with the nitrite to generate the N2; after completing the reaction, the pressure value is detected, and the nitrite content is calculated; the reaction of the reagent and the nitrite is performed in a closed reaction tank, and the N2 pressure is detected; according to the gas state equation, the pressure is proportional to the amount of the N2, and the amount of the N2 is proportional to the concentration of the nitrite, such that the nitrite concentration in the water body can be obtained by detecting the N2 pressure. With the present invention, the wide range detection of the nitrite can be achieved, and both the micro detection and the constant detection can be achieved. The present invention further discloses an automatic nitrite analyzer adopting the detection method.
Owner:LIHE TECH (HUNAN) CO LTD

A method for detecting red blood cell DNA damage signal and its application in lymphoma prognosis

The invention belongs to the field of medical biology. The application of red blood cell DNA damage signals in lymphoma prognosis includes the following steps: S1: prepare blood samples; S2: clean and process the control group and experimental group; S3: stain and process the second experimental group; S4: Resuspend the second control group; S5: Detect the third experimental group and the third control group by flow cytometry; S6: Analyze the results; S7, compare the changes in the proportion of the patient's own DNA damage signal, and simultaneously establish the relationship between healthy people and lymphoma The DNA damage signal database of the disease population can quickly and effectively compare the DNA damage signal of patients with lymphoma disease with the database to evaluate the prognosis of patients with lymphoma disease. Using trace blood samples and reagents, this method can quickly determine the DNA damage signal in early damaged red blood cells in lymphoma patients, which has the advantages of strong specificity and high sensitivity.
Owner:杭州太铭生物科技有限公司

Raman microscratch detection instrument based on distributive optical fiber amplifier

The invention relates to a Raman microscratch detection instrument based on a distributive optical fiber amplifier. The Raman microscratch detection instrument comprises five parts including a Raman spectrum display module, a laser module, a lens module, a Raman lens and a surface enhancing probe, wherein the Raman spectrum display module uses a CCD detector and is used for displaying the collected Raman spectrum information and guiding out the tested Raman spectrum information; the laser module is used for generating laser and uses a distributive Raman amplifier; the Raman lens is used for gathering the laser and emitting the laser to the surface enhanced probe; the surface enhanced probe is directly in contact with micro articles for detection; a main body of the surface enhanced probe is an optical fiber; a nanometer metal silver coating is formed on the surface of the optical fiber through a surface decomposition technology; the collection on the Raman spectrum of the trace stage sample to be tested can be realized.
Owner:TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products