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Magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and preparation method

A magnetic immunochromatography and quantitative detection technology, applied in the field of medical detection, can solve the problems of unresponsive detection results, weak protein binding, affecting sensitivity, etc., and achieve the effect of shortening detection time, high specificity and high sensitivity

Inactive Publication Date: 2018-05-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although colloidal gold is easy to prepare, can quickly label proteins, and has good visibility, it has poor stability and weak binding force with proteins
At the same time, for the immunochromatography system constructed with colloidal gold and other markers, its quantitative detection cannot reflect the most authentic detection results due to the influence of factors such as optical instruments, self-absorption, light scattering and self-interference, which affects the sensitivity of detection.

Method used

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  • Magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and preparation method
  • Magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and preparation method
  • Magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Preparation method of magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously

[0041] The preparation method of the test strip of the present embodiment may further comprise the steps:

[0042] A. Preparation of immunomagnetic beads:

[0043] Preparation of 2-morpholineethanesulfonic acid (MES) activation buffer: 0.01M MES buffer with pH5.5 (pH5.0-6.0 is applicable) is the activation buffer, add Tween-20, the final concentration is 0.05wt% , 0.22μm microporous membrane filtration sterilization, stored at 4 ℃ for later use, valid for two weeks.

[0044] Preparation of boric acid-borax (BS) coupling buffer: prepare 0.2M pH9.0 BS buffer with 0.05M borax and 0.2M boric acid at a ratio of 4:1 (v:v), dilute to 0.005M with ultrapure water , add Tween-20, the final concentration is 0.05%, 0.22μm microporous membrane filter sterilization, store at 4 ℃ for later use, valid for two weeks.

[0045] Preparation of bori...

Embodiment 2

[0063] Except in the preparation step of immunomagnetic beads: the addition amount of EDC and NHS is 5 times of the molar amount of carboxylation degree of the magnetic beads, and other steps are the same as in Example 1.

Embodiment 3

[0065] Except in the preparation step of the conjugate pad, the content of sucrose in the conjugate pad treatment solution used was 10 wt%, and other steps were the same as in Example 1.

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Abstract

The invention discloses a magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and a preparation method. The test strip is prepared by successively binding a sample pad, a combining pad, a nitrocellulose membrane and water absorbing paper to a bottom plate of the test strip and 2mm of adjacent parts are alternately overlaid one another, wherein the nitrocellulose membrane is pre-coated with a detection line of a CEA antibody and a CA19-9 antibody and a quality control line of a rabbit antimouse IgG antibody. Compared with existingtumor marker detection technologies, the magnetic immunochromatographic technology is creatively introduced into combined quantitative detection of CEA and CA19-9 to achieve microscale quantitative analysis of CEA and CA19-9, so that the detecting sensitivity is improved greatly; and based on double positive CEA and CA19-9, the occurrence rate and transfer rate of tumor can be further judged to improve early stage diagnostic rate of occurrence and transfer of colon cancer to achieve the purpose of immediate detection and quick diagnosis, and the test strip is simple to operate and low in cost, and can be widely popularized.

Description

technical field [0001] The invention relates to a method in the technical field of medical detection, in particular to a magnetic immunochromatographic test strip capable of simultaneously quantitatively detecting CEA and CA19-9 in blood and a preparation method thereof. Background technique [0002] Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker, which can reflect the existence of various tumors to people. CEA is a globulin first discovered in serum patients with colon cancer by Phil Gold and Samuel O. Freedman in 1965, and subsequently found in patients with gastric cancer, pancreatic cancer, lung cancer and breast cancer. The literature reports that its positive rate in colon cancer is about 60%. After surgical resection of colon cancer patients, blood CEA levels can drop to normal levels within 1-3 weeks. If the surgical resection is incomplete, the postoperative CEA remains positive, indicating that the prognosis of the patient is poor or metastasis h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 贾力韩龙宇李书慧王嘉宏
Owner FUZHOU UNIV
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