Test strip for simultaneously and quantitatively detecting NSE and S100B in blood and preparation method thereof

A quantitative detection and test strip technology, which is applied in the field of medical detection, can solve the problems of inability to realize ultra-early diagnosis, high professional level requirements, and expensive detection instruments, so as to achieve good clinical application significance, improve accuracy and sensitivity, and high sensitivity Effect

Pending Publication Date: 2019-11-19
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Description
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  • Application Information

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Problems solved by technology

Neuroimaging techniques (CT, MRI, TCD and PET) are the most widely used clinical diagnostic methods, which can effectively detect and distinguish between ischemic stroke and hemorrhagic stroke, but there are still some deficiencies and deficiencies, such as Different degrees of hysteresis make it impossible to achieve ultra-early diagnosis; the detection equipment is expensive, the penetration rate is not high, and the patient costs a lot; the detection operation is complicated and requires a high professional level, etc.

Method used

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  • Test strip for simultaneously and quantitatively detecting NSE and S100B in blood and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029]Example 1, this example provides a magnetic immunochromatographic test strip and a preparation method for quantitatively detecting NSE and S100B in blood at the same time.

[0030] Both the NSE paired antibody and the S100B paired antibody used in this example are monoclonal antibodies prepared by monoclonal antibody technology. The principle of double-antibody sandwich detection of NSE and S100B antigens is used to detect specimens. When the specimen to be tested contains NSE and S100 antigens, the antigens will first bind to the magnetic beads coupled with NSE antibody and the magnetic beads coupled with S100B antibody on the binding pad. , as the chromatography progresses, the conjugate moves forward to reach the S100B antibody detection line T2, the S100B antigen will combine with the coating antibody again to form a double antibody sandwich complex and gather at T2, and the conjugate moves forward to reach the NSE antibody At the detection line T1, the NSE antigen w...

Embodiment 2

[0053] Except in the preparation step of immunomagnetic beads: the addition amount of EDC and NHS is 5 times the molar amount of carboxylation degree of magnetic beads, other steps are the same as in Example 1.

Embodiment 3

[0055] Except that in the preparation step of the conjugate pad, the content of sucrose in the conjugate pad treatment solution used was 10 wt%, other steps were the same as in Example 1.

[0056] The use method of detection card of the present invention

[0057] 1. Add sample

[0058] Take out the test card for a single person from the packaging box, tear open the aluminum foil packaging bag, place it on a flat table, use a micropipette to pipette 70 μL of serum sample into the sample hole of the test card, and wait for the reaction to proceed for 20 minutes.

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Abstract

The invention belongs to the technical field of medical detection, and relates to a magnetic immunochromatographic test strip for simultaneously and quantitatively detecting NSE and S100B in blood anda preparation method thereof. The test strip is improved, and a magnetic immunochromatographic technology is creatively introduced into the combined detection of the NSE and the S100B for the first time, and by combining a magnetic signal detector, quantitative detection of NSE and the S100B for a single person can be realized simultaneously; and the test strip has the characteristics of being rapid, simple, practical, high in specificity and high in sensitivity, and can be used for remarkably improving the accuracy of cerebral apoplexy detection, that is, on the basis of double positive of NSE and S100B, the cerebral apoplexy occurrence rate can be further judged, so that the early diagnosis rate and prognosis monitoring of cerebral apoplexy can be improved, and the method has relativelyhigh clinical application significance.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to a magnetic immunochromatographic test strip and a preparation method for quantitatively detecting NSE and S100B in blood simultaneously. Background technique [0002] Stroke (stroke) is one of the three major causes of death for human beings, and the number of patients is increasing year by year. It has the characteristics of high fatality rate, high disability rate and high recurrence rate, which seriously endangers people's health and life safety. , financial and social have a huge burden. Ischemic stroke accounts for 60-80% of strokes, and the number of patients with hemorrhagic stroke is lower than that of ischemic stroke patients, but it has a higher disability rate and worse prognostic effect. Therefore, rapid and accurate early diagnosis is helpful to formulate treatment measures and reduce the mortality and disability rate of stroke. Neuroimaging techniques (CT...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/543
CPCG01N33/558G01N33/531G01N33/54326
Inventor 王莹杨明峰孙保亮范存东张宗勇袁慧
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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