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A time-resolved immunochromatographic test strip for quantitative detection of pepsinogen I and its preparation method

An immunochromatographic test strip and pepsinogen technology, applied in the field of clinical immunology detection, can solve the problems of unsuitability for single-unit and small batch detection, inability to achieve accurate quantification, low sensitivity, etc., and is suitable for large-scale production, Simple operation and high sensitivity effect

Active Publication Date: 2016-07-06
无锡市江原实业技贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Latex-enhanced immunoturbidimetric method is easy to operate and can be fully automated, but its sensitivity is not high, and accurate quantification cannot be achieved; ELISA method and time-resolved immunoassay are accurate in quantification, but manual operation is complicated, and it is not suitable for single-serving and For small batch testing

Method used

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  • A time-resolved immunochromatographic test strip for quantitative detection of pepsinogen I and its preparation method

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Effect test

Embodiment 1

[0028] The invention discloses a time-resolved immunochromatographic test strip for quantitative detection of pepsinogen I, the test strip comprising a plastic casing, a bottom plate, and a sample pad, a binding pad, a coating film and absorbent paper attached to the bottom plate and arranged alternately in sequence , the binding pad is coated with pepsinogen I monoclonal antibody I labeled with rare earth ion microspheres; the coating membrane is coated with a detection zone and a quality control zone, and the detection zone is fixed with a marker that recognizes different antigenic epitopes. Pepsinogen I monoclonal antibody II, the quality control band is immobilized with rabbit anti-mouse IgG antibody.

[0029] The combination pad is a polyester film, which can carry a sufficient amount of rare earth ion microspheres, and can release the microspheres rapidly after meeting a sample.

[0030] The coating membrane is a nitrocellulose membrane; the detection zone and the qualit...

Embodiment 2

[0048] The invention discloses a time-resolved immunochromatographic test strip for quantitative detection of pepsinogen I, the test strip comprising a plastic casing, a bottom plate, and a sample pad, a binding pad, a coating film and absorbent paper attached to the bottom plate and arranged alternately in sequence , the binding pad is coated with pepsinogen I monoclonal antibody I labeled with rare earth ion microspheres; the coating membrane is coated with a detection zone and a quality control zone, and the detection zone is fixed with a marker that recognizes different antigenic epitopes. Pepsinogen I monoclonal antibody II, the quality control band is immobilized with rabbit anti-mouse IgG antibody.

[0049] The combination pad is a polyester film, which can carry a sufficient amount of rare earth ion microspheres, and can release the microspheres rapidly after meeting a sample.

[0050] The coating membrane is a nitrocellulose membrane; the detection zone and the qualit...

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Abstract

The invention relates to the field of the clinic immunological detection, and in particular to a time resolution immunochromatographic test strip for quantitatively detecting pepsinogen I. The test strip comprises a plastic snap shell, a soleplate as well as a sample pad, a conjugate pad, a coating film and a piece of water absorption paper, which are sequentially adhered onto the soleplate in a staggering manner. The conjugate pad is coated with a pepsinogen I monoclonal antibody I marked by rare-earth ion microspheres; the coating film is coated with a detection band and a quality control band, a pepsinogen I monoclonal antibody II for recognizing different epitope is fixed on the detection band, and a rabbit anti-mouse IgG antibody is fixed on the quality control band. The invention also discloses a preparation method of the test strip. The two-antibody antigen sandwiched measuring technology and a time resolution immunochromatograhic technology are introduced to the detection of the pepsinogen I, and the single-person quantitative detection of the pepsinogen I is realized by combining a fluorescent detector; moreover, the sensitivity is high, the intra difference and inter difference are small, and great convenience is provided for the clinical use.

Description

technical field [0001] The invention relates to the field of clinical immunology detection, in particular to a time-resolved immunochromatographic test strip for quantitative detection of pepsinogen I and a preparation method thereof. Background technique [0002] Pepsinogen (PGI) is the inactive precursor of pepsin in gastric juice, which is divided into pepsinogen I (PGI for short) and pepsinogen II (PGII for short). Serum PG levels reflect the morphology and function of gastric mucosa in different parts. PGI is mainly secreted by the principal cells of fundic glands and cervical mucus cells. Diseases are closely related. The ratio of PGI / PGII also has high clinical value for judging the state and function of gastric mucosa. Determination of PGI content detects duodenal ulcer, atrophic gastritis, gastritis, gastric cancer and other gastrointestinal diseases, which are needed for clinical testing and physical examination screening. As a non-invasive method, PGI detection...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/533
CPCG01N33/533G01N33/558G01N33/573G01N33/577G01N2800/06
Inventor 黄飚周衍邹霈吕坤祥张珏张艺周彬
Owner 无锡市江原实业技贸有限公司
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