33results about How to "No reconstitution required" patented technology

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention provides a high-density liptein cholesterol test kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, alpha-cyclic glucan sulfate, dextran sulfate, pulullan, magnesium chloride, N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-sodium methylaniline, a surface active agent, and a preservative; and the reagent R2 contains the following components: 3-morpholinopropanesulfonic acid (MOPS) buffer solution, cholesterol esterase (CEH), cholesterol oxidase (COD), peroxidase (POD), 4-ampyrone,flavin adenine dinucleotide disodium, a surface active agent, a stabilizer, and a preservative. The invention also provides a preparation method and application of the kit. The kit is a liquid kit, which is high in stability, anti-interference capability and accuracy, and good in repeatability.

The invention provides an apolipoprotein A1 (ApoA1) determination kit, which contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazineethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative, and the reagent R2 is prepared from a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoA1 antibody coated latex particles, a surfactant, a stabilizer and a preservative. The invention also provides a preparation method and application of the kit, wherein the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The invention relates to the field of quality control of medical examination, in particular to the preparation of a single quality control substance of a hepatitis B e antigen. The hepatitis B virus eantigen is diluted to a required value by using a specific stabilizer to prepare the quality control product. The preparation process is simple, the production cost is low, added substances and contents are controllable, the product belongs to a liquid type, redissolution is not required, and the composition is stable. Through a biological traceability link program and reference method assignmentof the program, the product can be used as a third-party medical laboratory quality control product during the validity period to meet the needs of clinical infectious disease testing.

The invention provides a glutathione reductase assay kit, which comprises a reagent R1 and a reagent R2. The reagent R1 includes: 50-200 mmol / L of a Tris buffer solution, 0.5-2 mmol / L of EDTA, 1-5 kU / L of pyruvic carboxylase, 1-5 kU / L of ascorbic acid oxidase, 5-20 mg / L of potassium ferrocyanide, a surfactant and a preservative; the reagent R2 includes: 50-200 mmol / L of the Tris buffer solution, 0.5-2 mmol / L of EDTA, 2-10 mmol / L of GSSG, 0.1-0.5 mmol / L of NADPH, 1-15 g / L of a stabilizer, and 0.5-2 g / L of a preservative. The liquid assay kit has good stability and is strong in anti-interferenceeffect. The invention also discloses a preparation method and an application of the assay kit.

The invention provides a total antioxidant state determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains a 3-morpholine propanesulfonic acid (MOPS) buffer solution, sodium silicate, sodium pyrophosphate, ethylenediamine tetraacetic acid disodium salt, a surfactant and a preservative; and the reagent R2 comprises the 3-morpholine propanesulfonic acid (MOPS) buffer solution, potassium peroxydisulfate, 2, 2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS), potassium carbonate, the surfactant and the preservative. The invention alsoprovides a preparation method and application of the kit. The kit is a liquid kit with strong stability, strong anti-interference capability, high accuracy and good repeatability.

The invention discloses a serum iron determination kit. The kit comprises a reagent R1 and a reagent R2. The reagent R1 comprises a buffer solution, ascorbic acid, a de-interfering agent, a stabilizer, a surfactant, and a preservative. The reagent R2 comprises a buffer solution and bathophenanthroline disulfonic acid disodium salt. The invention further relates to a preparation method and application of the serum iron determination kit. The stable serum iron is a liquid double-reagent, reconstitution preparation is not required, and therefore the stable serum iron can be directly used when taken out from a bottle. The stabilizer is added in the reagent R1 to facilitate stability and reducibility maintenance of the ascorbic acid. The de-interfering agent is added in the reagent R1, thioureais mixed with a ascorbic acid solution, and therefore the R1 can be adopted as a masking agent as well as a reducing agent, and interference from other ions can be avoided. The reagent has high sensitivity. The kit is convenient to use, is high in specificity, is high in accuracy, is free of pollution to biochemical instruments, and can be further popularized in the market.

The invention provides an apolipoprotein B (ApoB) assay kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative; and the reagent R2 is prepared from the 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoB antibody coated latex particles, the surfactant, a stabilizer and the preservative. The invention also provides a preparation method and an application of the kit, and the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of an antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The present invention provides a pyruvic acid determination reagent box. The reagent box comprises a reagent R1 and a reagent R2. The reagent R1 comprises the following ingredients: reduced coenzyme I(NADH), a trihydroxymethyl aminomethane (Tris) buffer solution, a surface active agent, EDTA-Na2 and a preservative. The reagent R2 comprises the following ingredients: the trihydroxymethyl aminomethane (Tris) buffer solution, lactate dehydrogenase (LDH), the surface active agent, a stabilizer and the preservative. The present also provides a preparation method of the reagent box and applications. The reagent box is a high-stability, high-accuracy and good-repeatability liquid reagent box.

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

The invention provides a high-density lipoprotein cholesterol assay kit, the kit contains reagent R1 and reagent R2; the reagent R1 contains the following components: 3-morpholine propanesulfonic acid (MOPS) buffer solution, α-cyclic dextran Sulfate, dextran sulfate, pullulan, magnesium chloride, sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, surfactant, preservative; Reagent R2 : 3‑morpholinopropanesulfonic acid (MOPS) buffer, cholesterol esterase (CEH), cholesterol oxidase (COD), peroxidase enzyme (POD), 4‑aminoantipyrine, flavin adenine di Nucleotide Disodium, Surfactant, Stabilizer, Preservative. The invention also provides the preparation method and application of the kit. The kit is a liquid kit with strong stability, strong anti-interference ability, high accuracy and good repeatability.

The invention provides a pepsinogen I (PGI) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, NaCl , sodium hydroxide, surfactants, preservatives. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, mouse anti-human PGI antibody coated latex particles, suspending agent, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit can effectively avoid the interference of chylomicrons, and adopts different kinds of protective agents and suspending agents at the same time. It has strong stability, high sensitivity and good repeatability. Low cost liquid kit.

The invention provides an angiotensin converting enzyme assay kit, the kit contains a reagent R1 and a reagent R2, the reagent R1 comprises the following components: N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, sodium chloride, an ion activator, polyglycerol fatty acid ester, 4-hydroxy 2, 2, 6, 6-tetramethyl-1-oxo-piperidine (AAQ-2) and a preservative; and the reagent R2 is preparedfrom the following components: N-tri (hydroxymethyl) methyl-3-aminopropanesulfonic acid, FAPGG, a stabilizer and a preservative. The invention also provides a preparation method and application of thekit. The kit is a liquid kit with strong anti-interference capability, strong stability and good repeatability.

The invention provides a pepsinogen I (PGI) determination kit. The kit contains a reagent R1 and a reagent R2, the reagent R1 contains the following components: a piperazine 1, 4-diethylsulfonic acid(PIPES) buffer solution, NaCl, sodium hydroxide, a surfactant and a preservative, and the reagent R2 comprises the piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, mouse anti-human PGI antibody coated latex particles, a suspending aid, a stabilizer and the preservative. The invention also provides a preparation method and application of the kit. The kit can effectively avoid the interference of chyle particles, different types of protective agents and suspending agents are adopted at the same time, and the kit is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

The present invention provides a kit for measuring alpha-fetoprotein heterosome 3 (AFP‑L3), which contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine‑1,4‑diethanesulfonic acid (PIPES ) buffer, NaCl, surfactant, suspending agent, preservative. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, goat anti-human AFP-L3 antibody coated latex particles, goat anti-human AFP-L3 antibody coated magnetic nanoparticles, suspending agent, stabilizer Agents, preservatives. The invention also provides the preparation method and application of the kit. The kit can effectively replace the kit of a chemiluminescence immunoassay analyzer, and is a novel liquid kit with strong stability, high sensitivity, good repeatability and low cost. It is convenient for the development and application of the project in clinic.

The invention provides a direct bilirubin (DB) assay kit, the kit contains reagent R1 and reagent R2; the reagent R1 contains the following components: phosphate buffer, NaCl, N-acetylcysteine, sodium fluoride , Disodium EDTA, Ascorbate Oxidase, Surfactant, Preservative. Reagent R2: tris-hydrochloric acid buffer, bilirubin oxidase, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit uses different types of stabilizers and surfactants, which can effectively avoid the interference of blood lipids. It has strong anti-interference ability, high sensitivity and good stability. Low cost liquid kit.

The invention provides a sialic acid detection kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains the following components: a tris(hydroxymethyl)methyl aminomethane-hydrochloric acid (Tris-HCL) buffer solution, lactic dehydrogenase (LDH), N-acetylneuraminic acid aldolase, a reduced coenzyme I (NADH), a surfactant, a stabilizer, EDTA-Na2 and a preservative; and the reagentR2 contains the following components: a tris(hydroxymethyl)methyl aminomethane-hydrochloric acid (Tris-HCL) buffer solution, neuraminidase, a surfactant, a stabilizer and a preservative. A productionmethod and application of the kit are provided at the same time. The kit is a liquid kit with high stability, high anti-interference capability, high accuracy and good repeatability.

The invention provides a total bilirubin (TB) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: tris-hydrochloric acid buffer, NaCl, sodium cholate, lemon Acids, surfactants, preservatives. Reagent R2: tris-hydrochloric acid buffer, bilirubin oxidase, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit uses different types of stabilizers and surfactants, which can effectively avoid the interference of blood lipids. It has strong anti-interference ability, high sensitivity and good stability. Low cost liquid kit.

The invention provides a procalcitonin (PCT) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, MgCl2, chondroitin sulfate, hyaluronic acid, a surfactant and a preservative. And the reagent R2 is prepared from piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, goat anti-human PCT antibody coated latex particles, a suspending aid, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit can effectively avoid interference of chyle particles, latex particles of different specifications, novel surfactants and different protective agents and suspending agents are adoptedat the same time, and the kit is a liquid kit which is high in stability, high in sensitivity, good in repeatability and low in cost.

The invention discloses a sialic acid quality control substance and a preparation method thereof. The quality control substance comprises 40-200mmol / L of a buffer solution (with the pH value of 7.0-7.5), 1-300mg / L of polysialic acid sodium salt, 5-20ml / L of dimethyl sulfoxide, 0.1-3g / L of boric acid, 0.05-0.5mol / L of mannitol, 1-20mmol / L of glutathione, 1-20g / L of bovine serum albumin, 9g / L of sodium chloride and 0.1-1g / L of an antiseptic. The polysialic acid sodium salt is selected as a raw material and is better than sialic acid (N-acetylneuraminic acid) to represent the in vivo existence form of sialic acid, so the sialic acid quality control substance has the advantages of sufficient source, easy obtaining, liquid form and no re-dissolution.