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Anti-interference and stable serum direct bilirubin (enzymatic method) determination kit as well as preparation method and application thereof

A technology of bilirubin oxidase and kit, which is applied in the field of direct bilirubin assay kit preparation and direct bilirubin assay kit, and can solve the problems of poor anti-interference ability, influence, and easy contamination of instruments, etc.

Active Publication Date: 2021-04-27
中拓生物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diazonium salt method is widely used in clinical laboratories to detect bilirubin, but the method is susceptible to interference from many factors, and the reagents cannot be stable for a long time, which is affected to a certain extent in disease diagnosis
The vanadic acid oxidation method is easy to contaminate the instrument and affect other tests
The oxidase method has high specificity and high accuracy, but existing kits have shortcomings such as poor anti-interference ability, poor stability, and being easily affected by blood lipids. The present invention improves the shortcomings of the existing direct bilirubin kits to meet Requirements for clinical testing and chemical analysis

Method used

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  • Anti-interference and stable serum direct bilirubin (enzymatic method) determination kit as well as preparation method and application thereof
  • Anti-interference and stable serum direct bilirubin (enzymatic method) determination kit as well as preparation method and application thereof
  • Anti-interference and stable serum direct bilirubin (enzymatic method) determination kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A conventional direct bilirubin assay kit, which includes reagent R1 and reagent R2.

[0052] Reagent R1 contains the following components:

[0053] Phosphate buffer 25mmol / L;

[0054] NaCl 1g / L;

[0055] N-acetylcysteine ​​1g / L;

[0056] Sodium fluoride 0.1g / L;

[0057] Disodium EDTA 0.1g / L;

[0058] Ascorbate oxidase 1KU / L;

[0059] Sorbitan Laurate 1%;

[0060] Polyoxypropylene mannitol dioleate 1%;

[0061] proclin300 1 ml / L.

[0062] The pH of reagent R1 is 5.2.

[0063] Reagent R2 contains the following components:

[0064] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer 50mmol / L;

[0065] Bovine Serum Albumin (BSA) 5g / L;

[0066] Mannitol 10g / L;

[0067] Triton 100 1%;

[0068] Bilirubin oxidase 1KU / L;

[0069] proclin300 1ml / L.

[0070] The pH of reagent R2 is 9.0.

[0071] Wherein the percentages are volume ratios.

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Abstract

The invention provides a direct bilirubin (DB) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a phosphate buffer solution, NaCl, Nacetylcysteine, sodium fluoride, disodium ethylene diamine tetraacetate, ascorbic acid oxidase, a surfactant and a preservative; the reagent R2 comprises a trihydroxymethyl aminomethane hydrochloric acid buffer solution, bilirubin oxidase, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit adopts different types of stabilizers and surfactants, interference of blood fat can be effectively avoided; the liquid kit is high in interference resistance, high in sensitivity, good in stability and low in cost.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to a direct bilirubin determination kit, and also to a preparation method and application of the direct bilirubin determination kit. Background technique [0002] Bilirubin is a type of bile pigment, the main pigment in human bile. Bilirubin is the main metabolite of iron porphyrin compounds in the body, which is toxic and can cause irreversible damage to the brain and nervous system, but also has antioxidant functions that can inhibit the oxidation of linoleic acid and phospholipids. Bilirubin is an important basis for clinical judgment of jaundice and an important indicator of liver function. [0003] Direct bilirubin: Indirect bilirubin is high, direct bilirubin is high, indicating hepatocellular jaundice, liver cells are damaged, liver function is decreased, the liver cannot completely convert indirect bilirubin into direct bilirubin, and at the same t...

Claims

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Application Information

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IPC IPC(8): G01N33/72C12Q1/26G01N33/52
CPCC12Q1/26G01N33/52G01N33/728
Inventor 刘安娜
Owner 中拓生物有限公司
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