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A sensitive and stable serum procalcitonin assay kit and its preparation method and application

A procalcitonin and kit technology, which is applied in the preparation of procalcitonin assay kits and the field of procalcitonin assay kits, can solve the problem of being easily affected by chylomicrons (CM), inconvenient to store reagents, and of analytical sensitivity. Low problems, to achieve the effect of excellent performance indicators, high degree of automation, simple operation

Active Publication Date: 2020-10-09
中拓生物有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, reagents are inconvenient to store, and the price is high; colloidal gold colorimetry is simple to operate and produces fast results, but it can only be used for qualitative research and cannot Quantitative
Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. ) impact and other shortcomings, the present invention improves on the shortcomings of existing procalcitonin kits to meet the requirements of clinical testing and chemical analysis

Method used

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  • A sensitive and stable serum procalcitonin assay kit and its preparation method and application
  • A sensitive and stable serum procalcitonin assay kit and its preparation method and application
  • A sensitive and stable serum procalcitonin assay kit and its preparation method and application

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Embodiment 1

[0040] A conventional assay kit for procalcitonin, which includes reagent R1 and reagent R2.

[0041] Reagent R1 contains the following components:

[0042]

[0043] Reagent R1 has a pH value of 7.0.

[0044] Reagent R2 contains the following ingredients:

[0045]

[0046]

[0047] Reagent R2 has a pH of 6.8.

[0048] Wherein said percentage is volume ratio. The mass ratio of latex particles in reagent R2 with particle diameters of 50nm, 100nm and 150nm is 1:1:1.

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Abstract

The invention provides a procalcitonin (PCT) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, MgCl2, chondroitin sulfate, hyaluronic acid, a surfactant and a preservative. And the reagent R2 is prepared from piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, goat anti-human PCT antibody coated latex particles, a suspending aid, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit can effectively avoid interference of chyle particles, latex particles of different specifications, novel surfactants and different protective agents and suspending agents are adoptedat the same time, and the kit is a liquid kit which is high in stability, high in sensitivity, good in repeatability and low in cost.

Description

technical field [0001] The present invention relates to the technical field of biochemical reagent assay, in particular to a procalcitonin assay kit, and also to a preparation method and application of the procalcitonin assay kit. Background technique [0002] PCT is the precursor of calcitonin (CT), a glycoprotein of 116 amino acids, with a half-life of about 20-24 hours in the human body and good stability; its content in normal human serum is extremely low, less than 0.5 ng / mL. In addition to thyroid trauma or tumors, it is significantly elevated in the serum of patients with systemic inflammatory response syndrome (SIRS), blood diseases, acute and chronic pneumonia, acute pancreatitis, active hepatitis, trauma, etc., especially for SIRS / blood diseases, PCT is A very sensitive and specific serological marker. However, it remains at a low level when the virus is just infected, and PCT can increase in the early stage of severe bacterial infection (2-3 hours later), so it h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/54346G01N33/54393G01N33/74G01N2333/585
Inventor 刘安娜
Owner 中拓生物有限公司
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