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A kind of sialic acid quality control product and preparation method thereof

A quality control product, sialic acid technology, applied in the field of medical testing, can solve the problems of lack of international standards, difficulties in popularization and application, inconsistent test results, etc., and achieve the effect of easy acquisition, strong anti-interference ability and long storage time

Active Publication Date: 2016-08-24
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, enzymatic sialic acid detection kits have appeared at home and abroad, but due to the lack of international standards, the detection results may be inconsistent with different manufacturers and reagents, which has brought great challenges to the promotion and application of this project. difficulty

Method used

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  • A kind of sialic acid quality control product and preparation method thereof
  • A kind of sialic acid quality control product and preparation method thereof
  • A kind of sialic acid quality control product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]

[0022] The preparation method of this sialic acid quality control product, comprises the following steps:

[0023] (1) Dissolve 1g of boric acid and 0.1mol mannitol in 20ml of dimethyl sulfoxide, mix well, then add 50mg of polysialic acid sodium salt, mix well;

[0024] (2) Prepare 1L of 50mmol hydroxyethylpiperazine ethylsulfuric acid buffer solution, adjust the pH to 7.0, add 5mmol glutathione, 10g bovine serum albumin, 9.2g sodium chloride, 0.1g sodium azide, mix well ;

[0025] (3) Mix the above (1) and (2) solutions at a volume ratio of 20:1000.

Embodiment 2

[0027]

[0028]

[0029] The preparation method of this sialic acid quality control product, comprises the following steps:

[0030] (1) Dissolve 0.5g of boric acid and 0.2mol mannitol in 10ml of dimethyl sulfoxide, mix well, then add 100mg of polysialic acid sodium salt, mix well;

[0031] (2) Prepare 1L of 100mmol tris hydrochloride buffer solution, adjust the pH to 7.5, add 20mmol glutathione, 20g bovine serum albumin, 9.1g sodium chloride, 0.1g Proclin 300, mix well ;

[0032] (3) Mix the above (1) and (2) solutions at a volume ratio of 10:1000.

[0033] The results of quality control products prepared in the above embodiments of the present invention stored in an environment of 2-8°C for 1 year are shown in Table 1 below:

[0034] Table 1

[0035] month

Example 1 (mg / L)

Example 2 (mg / L)

0

49.9

101.5

3

50.3

103.2

6

49.1

102.5

9

48.8

97.9

12

47.1

98.2

[0036] It can be known from ...

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Abstract

The invention discloses a sialic acid quality control substance and a preparation method thereof. The quality control substance comprises 40-200mmol / L of a buffer solution (with the pH value of 7.0-7.5), 1-300mg / L of polysialic acid sodium salt, 5-20ml / L of dimethyl sulfoxide, 0.1-3g / L of boric acid, 0.05-0.5mol / L of mannitol, 1-20mmol / L of glutathione, 1-20g / L of bovine serum albumin, 9g / L of sodium chloride and 0.1-1g / L of an antiseptic. The polysialic acid sodium salt is selected as a raw material and is better than sialic acid (N-acetylneuraminic acid) to represent the in vivo existence form of sialic acid, so the sialic acid quality control substance has the advantages of sufficient source, easy obtaining, liquid form and no re-dissolution.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a sialic acid quality control product and a preparation method thereof. Background technique [0002] N-Acetylneuraminic Acid (NANA), commonly known as Sialic acid (SA), is one of many natural neuraminic acid derivatives, and its parent structure is an endocyclic amino acid composed of nine carbon atoms , is a general term for a family of compounds, which got its name because it was originally isolated from submandibular gland mucin. Sialic acid is an important component of glycoconjugates and polysialic acid, widely distributed in the terminal of oligosaccharide chains in biological systems. Natural sialic acid exists in the form of α-glycosidic bonds, mainly through α(2-3) or α(2-6) glycosidic bonds and galactosides to form glycoconjugates (including glycoproteins, oligosaccharides, etc.) and glycolipid bodies), or form linear polysialic acid through α(2-8), α(2-9) or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96
CPCG01N33/96
Inventor 沃燕波邹炳德邹继华
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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