A kind of sialic acid quality control product and preparation method thereof
A quality control product, sialic acid technology, applied in the field of medical testing, can solve the problems of lack of international standards, difficulties in popularization and application, inconsistent test results, etc., and achieve the effect of easy acquisition, strong anti-interference ability and long storage time
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Embodiment 1
[0021]
[0022] The preparation method of this sialic acid quality control product, comprises the following steps:
[0023] (1) Dissolve 1g of boric acid and 0.1mol mannitol in 20ml of dimethyl sulfoxide, mix well, then add 50mg of polysialic acid sodium salt, mix well;
[0024] (2) Prepare 1L of 50mmol hydroxyethylpiperazine ethylsulfuric acid buffer solution, adjust the pH to 7.0, add 5mmol glutathione, 10g bovine serum albumin, 9.2g sodium chloride, 0.1g sodium azide, mix well ;
[0025] (3) Mix the above (1) and (2) solutions at a volume ratio of 20:1000.
Embodiment 2
[0027]
[0028]
[0029] The preparation method of this sialic acid quality control product, comprises the following steps:
[0030] (1) Dissolve 0.5g of boric acid and 0.2mol mannitol in 10ml of dimethyl sulfoxide, mix well, then add 100mg of polysialic acid sodium salt, mix well;
[0031] (2) Prepare 1L of 100mmol tris hydrochloride buffer solution, adjust the pH to 7.5, add 20mmol glutathione, 20g bovine serum albumin, 9.1g sodium chloride, 0.1g Proclin 300, mix well ;
[0032] (3) Mix the above (1) and (2) solutions at a volume ratio of 10:1000.
[0033] The results of quality control products prepared in the above embodiments of the present invention stored in an environment of 2-8°C for 1 year are shown in Table 1 below:
[0034] Table 1
[0035] month
Example 1 (mg / L)
Example 2 (mg / L)
0
49.9
101.5
3
50.3
103.2
6
49.1
102.5
9
48.8
97.9
12
47.1
98.2
[0036] It can be known from ...
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