Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of glycosylated serum protein assay kit and its preparation method and application

A glycated serum protein and a kit technology are applied to the glycated serum protein assay kit. The preparation field of the glycated serum protein assay kit can solve the problems of analyzer pollution, high price, time-consuming and the like, and achieves convenient use and specificity. High performance and high accuracy

Active Publication Date: 2021-03-02
中拓生物有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affinity chromatography is time consuming and difficult to automate
The NBT method is a commonly used quantitative method for glycated serum protein in China. Nitrotetrazolium blue is selected as the chromogen in the NBT method, and the colorimetric method is used for the determination of glycated serum protein. This method is fast and sensitive, but the stability of the kit is poor, and NBT is water-soluble Not good, it is very easy to adsorb on the cuvette and pipeline of the biochemical analyzer, causing pollution to the analyzer
The protease method has the advantages of good specificity and no pollution to biochemical analyzers, but the reagents rely on imports, and the reagents are generally freeze-dried and expensive
If a conventional protease system is used for the enzymatic hydrolysis reaction of glycated serum protein, it will take tens of minutes or even several hours to complete the enzymatic hydrolysis, and the enzymatic hydrolysis method simply increasing the concentration of protease greatly increases the reaction cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of glycosylated serum protein assay kit and its preparation method and application
  • A kind of glycosylated serum protein assay kit and its preparation method and application
  • A kind of glycosylated serum protein assay kit and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A conventional glycosylated serum protein assay kit includes reagent R1 and reagent R2.

[0035]

[0036]

[0037] Reagent R2:

[0038]

[0039] The reagents R1 and R2 described in this example need to prepare tris buffer (Tris) first, adjust the pH with hydrochloric acid, adjust to an appropriate pH value, and then add other substances to dissolve to obtain the assay reagent.

Embodiment 2

[0041]

[0042] Reagent R2:

[0043]

[0044]

[0045] The preparation method is the same as in Example 1.

Embodiment 3

[0047]

[0048] Reagent R2:

[0049]

[0050] The preparation method is the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a glycated serum protein determination kit. The kit contains a reagent R1 and a reagent R2; the reagent R1 contains Tris buffer solution, trypsin, peroxidase, an anti-interference substance, a protein denaturant, an ionic liquid, CaCl2, a surfactant and a preservative; and the reagent 2 contains the Tris buffer solution, fructosaminase, 4-aminoantipyrine, sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate (TOOS), a surfactant, a stabilizer and a preservative. The kit disclosed by the invention is a liquid kit high in accuracy and good in stability. The invention also discloses a preparation method and application of the kit.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a test kit for glycosylated serum protein, and also relates to a preparation method and application of the test kit for glycosylated serum protein. Background technique [0002] Glycated serum protein is a non-enzymatic glycation reaction between serum glucose and the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. The binding process of various serum proteins and sugar is basically the same. The non-ionic ε or α-amino group on the protein molecule and the carboxyl group on the aldose form an unstable compound, namely Schiff's base, which can be dissociated It is a protein and aldose, and can be rearranged through Amadori transposition to generate a relatively stable amino-1-deoxy-2-ketose compound, called ketosamine (Ketosamine), because its structure is similar to fructosamine (Fructosamine, FMN) Therefore, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/68
CPCG01N21/78G01N33/68
Inventor 王飞刘安娜张强
Owner 中拓生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products