The invention discloses a rapid and low-cost method for separating a PCR template from chicken blood. The rapid and low-cost method for separating the PCR template from the chicken blood comprises thesteps that A,
blood corpuscle nucleuses are separated from a blood sample, specifically, a fresh and frozen
anticoagulant chicken blood sample is added into a 1.5 mL EP
pipe, and 4000rpm
centrifugation is carried out for one minute and supernatant is removed; and 500 [mu]l
sterile water is added into the
pipe to stand for one minute, 9500rpm
centrifugation is carried out for three minutes, and the supernatant is abandoned; B, the corpuscle nucleuse dissociation is carried out, specifically, a 3-4% chelated type
ion exchange resin solution shaken up by the 200 [mu]l
sterile water is added intosediment by using a spearhead with the
cut tip part, after mixing, water bath at 55 DEG C-57 DEG C is carried out for 30 minutes, then, a
dry heat meter is placed in, and heating is carried out at 100 DEG C for 8 minutes; and C, PCR template separation is carried out, specifically, 9500rpm
centrifugation is carried out for two minutes, and the supernatant is taken as a PCR template. Compared witha template obtained by using an existing traditional
DNA extraction method, the PCR template obtained by using the rapid and low-cost method for separating the PCR template from the chicken blood hassame effects for PCR technology detecting, the operation is simpler and easier to operate, the time-consuming is short (within one hour), the cost is low (about 0.31 yuan / sample), and the PCR template is suitable for various
rapid DNA molecular detection experiments carried out by using PCR technology.