30results about How to "Guaranteed to successfully build" patented technology

The invention provides a method for constructing an open-plot flower mixed combination landscape, which includes: performing simulation sowing in a lab, dividing seeds into large-sized, medium-sized and small-sized seeds, wherein a long axis of three dimensions of each seed serves as the size of the seed, the large-sized seed is larger than 5.0mm in size, the medium-sized seed ranges from 2.0mm to 5.0mm in size, and the small-sized seed is smaller than 2.0mm in size; and then sowing the seeds on an open plot, actually sowing each type of large-sized flower seeds in the open plot by a certain amount twice to three times that of needed plants, and actually sowing each type of medium-sized or small-sized seeds by a certain amount eight times to twelve times that of the needed plants. Therefore, the amount of seedlings needed in the landscape can be guaranteed so as to ensure construction of the effective landscape according to the seed germinating amount.

The invention discloses construction of a 21ShRNA fluorescent expression vector of a long-term silencing esophageal cancer cell HIF-1Alpha gene. The construction method comprises the following steps of: acquiring HIF-1Alpha gene ID NM_001530.3 from NCBI (National Center of Biotechnology Information), designing 21 oligonucleotide siRNA interference sequences by software forecasting, designing a shRNA-encoded DNA sequence according to plasmid insertion sites and joint structures, and connecting pEZsiRNA6.1 vectors to obtain pEZsiRNA6.1hif-1Alpha recombinant plasmid of the long-term silencing HIF-1Alpha gene. The invention designs the HIF-1Alpha interference sequence and constructs the shRNA long-term interference fluorescence expression vector by utilizing a shRNA principle, can obtain a stable cell line expressed by long-term silencing HIF-1Alpha protein after cell transfection, screening and identification and provide a new material for new drug development and gene therapy which takeHIF-1Alpha as a target.

Embodiments of the invention disclose a method and a device for establishing a Bluetooth voice link, a storage medium and a mobile terminal. The method comprises the following steps of acquiring identification information of a Bluetooth device when an audio data playing instruction is received; determining a target encoding and decoding mode corresponding to the Bluetooth device according to the identification information; and establishing the Bluetooth voice link with the Bluetooth device on the basis of the target encoding and decoding mode. According to the embodiments of the invention, through adoption of the technical scheme, the technical problem of incapability of outputting the audio data by the Bluetooth device due to the establishment failure of the Bluetooth voice link between the mobile terminal and the Bluetooth device can be solved, and the successful establishment of the Bluetooth voice link between the mobile terminal and the Bluetooth device is effectively ensured.

The invention discloses a method, a system and a device for communicating between a trunking communication system and a conventional wireless system, wherein the device comprises the following steps: a relay of a conventional end dials number according to prestored identification code of a relay of a trunking end so as to establish wireless link with relay of the trunking end; first control information and voice are generated when the relay of the conventional end receives the carrier wave transmitted by the terminal of a conventional wireless system, and are transmitted to the relay of the trunking end through the wireless link; the relay of the trunking end transmits the voice to trunking users according to the first control information; the received voice of the trunking users are transmitted to the relay of the conventional end through the wireless link, and when the relay of the conventional end receives the voice, the voice is transmitted to conventional users; and when no voice is transmitted to the relay of the conventional end, the relay of the conventional end disconnects the voice call. The invention can realize portable wireless interconnection between the MPT (Microprogramming technique) trunking and the conventional terminal.

A method and a device for establishing a WLAN link. The method comprises: a first network device using an initial bandwidth to broadcast a probe request; detecting whether a probe response corresponding to the probe request and sent by a second network device has been received and, if the probe response has not been received, using an adjusted bandwidth to broadcast the probe request; and then beginning to re-execute from the aforementioned step of detecting whether a probe response corresponding to the probe request and sent by second network device has been received, the adjusted bandwidth being a smaller bandwidth than the previously used bandwidth; and, if a probe response is received, then establishing a WLAN link with the second network device, the bandwidth of the WLAN link being the bandwidth used when receiving the probe response. The solution provided in the embodiments of the present application uses a smaller adjusted bandwidth to re-send a probe request when a probe response corresponding to the probe request has not been received, and thereby expands the scope of the probe, ensuring successful establishment of a WLAN link.