Saccharopolyspora pogona delta Clu13-MmsA and construction method and application thereof

Abstract

The invention provides Saccharopolyspora pogona delta Clu13-MmsA and a construction method and application thereof. The Saccharopolyspora pogona delta Clu13-MmsA is preserved in the China Center for Type Culture Collection, and the preservation number of the strain is CCTCC NO: M2021825. The construction method of the Saccharopolyspora pogona delta Clu13-MmsA is easy and convenient to operate, the Saccharopolyspora pogona delta Clu13-MmsA is used for producing butenyl spinosad through fermentation, the yield of the butenyl spinosad is increased by 10.1 times compared with that of Saccharopolyspora pogona original bacteria, and meanwhile the yield of the butenyl spinosad is far higher than that of an S.pogona-MmsA strain.

Description

technical field [0001] The present invention relates to a strain of Saccharopolyspora mentabilis and its construction method and application, in particular to a strain of Saccharopolyspora mentasyn ΔClu13-MmsA with high production of butenyl spinosad and its construction method and application. Background technique [0002] Crop pests are one of the main obstacles to the sustainable development of modern agriculture in my country. Traditional chemical pesticides can resist the invasion of pests, but they are easy to remain, which will pollute and damage the environment and cause food safety problems. Biopesticides are gradually being valued because of their strong selectivity, non-toxic and harmless, and not easy to produce resistance. With the improvement of people's awareness of ecological environment and food safety, the demand for green biopesticides for environmental and crop safety is also increasing. [0003] Butenyl-spinosyn (butenyl-spinosyn), which is produced by...

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Problems solved by technology

Subsequently, in order to construct high-yielding spinosyn engineering strains, researchers conducted extensive research, including the two-component system SenX3-RegX3 (see "Rang J, He H, ChenJ, et al. SenX3-RegX3, an Important Two-Component System, Regulates Strain Growth and Butenyl-spinosyn Biosynthesis in Saccharopolyspora pogona.iScience.2020; 23(8):101398"), TetR family regulator (see "He H, Yuan S, Hu J, et al. Effect of theTetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona. Microb Cell Fact.2020; 19(1):27"), GDP-fucose synthase (see "Peng Shengnan, He Haocheng, Yuan Shuangqin et al. fcl gene pair Effects of butenyl spinosyn biosynthesis and growth and development of Saccharopolyspora palpitaciens. Chinese Journal of Bioengineering, 2019, 35(09):1662-1675") and other polyketide gene clusters (see "Rang J, Li Y , Cao L, et al. Deletion of a hybrid NRPS-T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl-spinosyn biosynthesis and growth development. MicrobBiotechnol. Breakthrough, the production of butenyl spinosyns of existing engineering strains of S.

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