Saccharopolyspora tuberosa engineering strain with doubled pII gene as well as construction method and application of saccharopolyspora tuberosa engineering strain
A technology of Saccharopolyspora spp. and engineering strains, which is applied in the field of S. saccharopolyspora spp. engineering strains and its construction, and can solve the problems of long fermentation period, low yield of butenyl pleocidins, and many by-products of fermentation broth.
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[0042] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0043] See Table 1 for the relevant descriptions and sequences of the strains, plasmids and primers used in the specific embodiments of the present invention.
[0044] (1) Medium formula and strain culture conditions
[0045] (1) LB medium (100 mL): Mix Yeast extract 0.5 g, Tryptone 1 g and NaCl 1 g, dilute to 100 mL with ultrapure water, adjust the pH value of the solution to 7.0, and add 1.6 g agar powder, sterilized by high-pressure steam at 121°C for 20 min, and set aside;
[0046] (2) CSM Streptomyces Activation Medium (100 mL): mix 4.5 g of TSB, 1 g of Glucose, 0.9 g of Yeast extract and MgSO 4 •7H 2 O 0.22 g, mixed with ultrapure water to 100 mL, 1.6 g of agar powder was added to the solid medium, sterilized by high-pressure steam at 115 °C for 30 min, and set aside;
[0047] (3) TSB medium (100 mL): Tryptic Soy Broth 3 g, dilute to ...
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