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Application of B19 virus VP1 unique protein to construct viral nephropathy model

A unique domain protein, viral technology, applied in the direction of peptide/protein components, medical preparations containing active ingredients, pharmaceutical formulations, etc., to achieve good operability, high success rate and good safety.

Inactive Publication Date: 2014-10-08
FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. B19 virus and its pathogenicity

Method used

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  • Application of B19 virus VP1 unique protein to construct viral nephropathy model
  • Application of B19 virus VP1 unique protein to construct viral nephropathy model
  • Application of B19 virus VP1 unique protein to construct viral nephropathy model

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0031] The present invention adopts the feasibility of expressing and purifying the B19 virus VP1 unique region protein in the process of "Expression and Purification of Human Parvovirus B19 VP1 Unique Region Protein" disclosed in "Journal of Cellular and Molecular Immunology" by Li Zhihong and Zhang Guocheng in 2007. The scheme is implemented as follows.

[0032] 1. B19 virus VP1 unique region protein injection mice

[0033] (1) Select 6-week-old BALB / c mice;

[0034] (2) 12 μg of VP1 unique domain protein (concentration: 1.2 mg / mL) was injected into the tail vein each time with B19 virus VP1 unique domain protein, and the control group was injected with normal saline;

[0035] The molecular weight of the B19 virus VP1 unique region protein is 22kDa, and the purity is 96.7%;

[0036] (3) ...

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Abstract

The invention discloses application of B19 virus VP1 unique protein to construct a viral nephropathy model. The application comprises: injecting purified B19 virus VP1 unique protein into BALB / c mouse via tail vein for three times with the interval of 10 day, and taking a sample for correlated detection after 10 days of performing the final injection; and performing analysis on kidney tissue respectively by utilizing a light microscope, an electron microscope and TUNEL immunofluorescence, and performing routine analysis on urine and peeing. The results show that B19 virus VP1 unique protein is capable of causing change of kidney tissue and urine, and therefore the B19 virus VP1 unique protein can be used to successively construct the viral nephropathy model. There is certain risk by using a common active virus to establish the viral nephropathy model through infection, while the technical scheme of utilizing B19 virus VP1 unique protein to construct the viral nephropathy mode has the characteristics of good safety, good maneuverability and high success rate.

Description

technical field [0001] The invention belongs to the technical field of constructing viral nephropathy models, and relates to the application of B19 virus VP1 unique region protein to construct viral nephropathy models. Background technique [0002] 1. Research status of viral nephropathy [0003] Viral nephropathy is a group of kidney diseases caused by viral infection. The common pathogens include hepatitis B virus (HBV), hepatitis C virus (HCV), B19 virus, HIV and so on. Viral nephropathy caused by B19 virus infection reported abroad includes glomerulonephritis, nephrotic syndrome, renal insufficiency and other diseases. Recently, B19 virus infection is considered to be closely related to collapsing glomerulopathy (CG), which has severe clinical manifestations, poor treatment effect, rapid disease progression, and poor prognosis. The detection rates of B19 virus DNA in kidney tissue and peripheral blood of CG patients were respectively 78% and 87%. Domestic Hao Wen et a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16
Inventor 聂晓晶马雷丁晓华
Owner FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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