30results about How to "Good tolerance to bile salts" patented technology

The invention relates to a feed for early weaned piglets, which belongs to the field of the feed. Main ingredients of the feed comprise expanded corn, full-fat soybean, fat powder, steam fish meal, acidified whey powder, fermented soybean meal, a complex enzyme preparation, a micro-ecological preparation, amino acids, complex vitamins, complex trace elements and the like. The digestive physiological characteristics of the weaned piglets are fully considered on the selection and the processing of raw materials, thereby greatly improving the palatability and the digestion rate of the feed; simultaneously, through the synergistic compatibility of an egg yolk antibody, lactein, sodium humate and the complex micro-ecological preparation, the diarrhea rate of the weaned piglets is reduced, the effects are superior to those of adding antibiotics into the feed, and the feed is the green, environmentally-friendly and nuisanceless feed. An animal feeding experiment proves that the feeding effects are ideal, the palatability is good, the piglets after weaning are high in average feed intake, fast in daily weight gain and low in diarrhea rate, and the feed has a good promoting effect for growth and health conditions of the weaned piglets and can meet the demands of most of raisers at present.

Belonging to the premix field, the invention relates to an additive premix for laying hens. The premix of the invention mainly contains: soybean meal, calcium hydrogen phosphate, stone powder, salt, compound vitamin, nicotinic acid, D-calcium pantothenate, biotin, choline chloride, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, potassium iodide premix, sodium selenite premix, ethoxy quinoline, phytase, microecologics, xylo oligosaccharide, bentonite and rice husk. With xylo oligosaccharide and a plurality of probiotics and without antibiotic, the premix in the invention hasa reasonable formula and high viable bacteria content. Animal feeding experiments prove that the additive premix in the invention has ideal feeding effects. The ammonia concentration, the death and culling rate, and the broken egg rate can be reduced, while the laying rate and egg weight are enhanced. The composite premix provided in the invention can well promote the laying performance and health status of laying hens, and meet the needs of mass raising households, and is suitable for green and healthy culture so as to produce pollution-free egg products.

The invention relates to compound probiotics and a preparation method thereof. The compound probiotics comprise ten or more probiotics and two or more prebiotics. The invention also provides the preparation method of the compound probiotics. The preparation method comprises the following steps: firstly, mixing the compound probiotics according to the proportion, passing through a sieve with 80 to 120 meshes, then taking siftage and weighing; secondly, mixing the weighed materials; thirdly, tabletting mixed materials. According to the compound probiotics and the preparation method thereof disclosed by the invention, by scientifically mixing the probiotics with the prebiotics, the efficacy of the compound probiotics is effectively improved, living cell concentration is high, and the efficacy realizing time of the probiotics is shortened; compared with other products in the market, the compound probiotics have the advantages that acid and bile salt resisting capabilities are good; if the compound probiotics are administrated for one month, the improvement rate of constipation reaches 100 percent, the improvement rate of chronic colitis reaches 100 percent, the positive-to-negative rate of helicobacter pylori reaches 82 percent, the rehabilitation rate of chemical liver injury reaches 88 percent, the rehabilitation rate of anaphylactic rhinitis is 91 percent, the rehabilitation rate of female vaginitis reaches 93 percent, the improvement rate of phlegm-dampness constitution reaches 100 percent, the improvement rate of damp-heat constitution reaches 100 percent, and the improvement rate of acnes reaches 98 percent.

The invention discloses a method for preparing butyric acid bacteria powder by microencapsulated propagation culture and application, which belong to the technical field of microbial agents. In the invention, an initial strain is clostridium butyricunm JSIM-MCB20040312 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.1647; the butyric acid bacteria powder is prepared by the steps of activation of butyric acid bacteria seeds, microencapsulated embedding of the butyric acid bacteria seeds, viable counting and preparation of butyric acid bacteria powder, wherein the concentration of the obtained bacteria powder is not less than 1.0*1011 / g. The viable organism propagation capacity of the butyric acid bacteria subjected to microencapsulated embedding is enhanced; a strict anaerobic environment created by liquid sealing of nitrogen gas or carbon diode and other inert gases or paraffin is avoided; a high-concentration bacteroid can be obtained without introducing air into a general fermentation tank for culturing; the production cost is low and the requirement for fed microbial additives on viable count can be met; the butyric acid bacteria powder also has the characteristics of heat resistance, acid resistance and favorable entericsolubility and is suitably used as animal intestine probiotics; and intestinal diseases of livestock and poultry farming animals such as chicken, pigs and the like are effectively prevented and the growth of the bred animals is promoted.

The invention discloses a method for preparing three-layer embedded bifidobacterium microcapsules, and the method comprises the following steps of: squeezing bifidobacterium glue solution into soybean oil containing an emulsifier by a pressure nozzle, thoroughly stirring, emulsifying, and then adding a calcium chloride solution and standing; after layering through standing, pouring the supernatant soybean oil out and centrifuging to obtain wet capsules, and then washing the wet capsules thoroughly by using normal saline, thereby obtaining the bifidobacterium microcapsules; mixing the bifidobacterium microcapsules with a two-layer embedding solution, oscillating and stirring, centrifuging, and then collecting the two-layer embedded bifidobacterium microcapsules; adding Tween-80 to palm oil, mixing evenly and thoroughly, and sterilizing, thereby obtaining a three-layer embedding solution; mixing the two-layer embedded bifidobacterium microcapsules with the three-layer embedding solution, oscillating and stirring, centrifuging and then collecting the three-layer embedded bifidobacterium microcapsules. The bifidobacterium is protected by the three-layer embedded microcapsule technology; and as a result, the storage periods of the bifidobacterium preparation and the product containing the bifidobacterium can be prolonged.

The present invention discloses Lactobacillus paracasei ZLP019, which has a preservation number of CGMCC No.11532, and has characteristics of good acid resistance, good cholate resistance and good bacterial inhibition. According to the present invention, with the application of the Lactobacillus paracasei ZLP019 to prepare the freeze-drying preparation, the live bacteria survival rate is high, and the preparation is conveniently used in feed enterprises and farms; and after the preparation is directly added to sow feed according to the ratio requirement, the reproductive performance of sows can be improved, the daily weight gain and the survival rate of suckling piglets can be improved, the intestinal health of sows can be improved, the harmful bacteria emission in feces can be reduced, and the environmentally friendly breeding environment can be easily achieved.

The invention relates to a preparation technology of a bacillus feed additive agent, belonging to the field of feed additive agent. The preparation technology comprises the following steps: 1) culturing bacillus to obtain a seed liquid; 2) carrying out liquid fermentation culture of bacillus with the inoculation amount of the seed liquid is 3-10% and until the microbial content reaches 1-2*10<10>cfu / ml; 3) collecting thalli by centrifugation; 4) freeze-drying thalli: adding a freeze-drying protectant in the collected thalli, putting the mixture in a freeze dryer for vacuum freeze-drying to obtain freeze-dried bacterial powder; and 5) adding auxiliary materials in the freeze-dried bacterial powder to obtain the bacillus feed additive agent. The feed additive agent obtained by the preparation technology has good probiotic functions of stress resistance and preventing pathogenic bacteria such as gastric acid resistance, cholate resistance, high temperature resistance, antibiotic tolerance and the like, can be used in combination with antibiotics. The prepared feed additive has high viable bacteria content, long -term storage, and obvious and stable application effects.

The invention relates to a Bacillus coagulans-Lactobacillus casei-Lactobacillus plantarum probiotic inoculum and preparation thereof. The solid fermentation probiotic inoculum belongs to the technical field of feed microbes. The invention relates to an application technology which is implemented by carrying out solid state fermentation on bean pulp, bran and corn flour used as matrixes by using Bacillus coagulans HM-09, Bacillus licheniformis BL-09, Lactobacillus casei HM-09 and Lactobacillus plantarum HM-10, wherein the collection number of the Bacillus licheniformis BL-09 is CGMCC No.5686. The preparation method comprises the following steps: inoculating Bacillus coagulans, Lactobacillus casei and Lactobacillus plantarum into a solid culture medium, carrying out mixed fermentation for 18-28 hours, adding Bacillus licheniformis BL-09, and continuing fermentation for 20-28 hours to obtain the product. The product has special acid aroma, the viable count of the bean pulp-bran-corn flour fermented product is high, and the degradation rate of the bean pulp protein is high. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention discloses an application of lactobacillus fermentum CQPC04, the preservation number of which is CGMCC NO, 14493, in preparing food or drugs for improving ulcerative colitis. The application range of the lactobacillus fermentum CQPC04 is expanded, the development utilization value is improved, and a new hope is brought to improve the ulcerative colitis.

The present invention provides a lactobacillus paracasei subsp. paracasei. The lactobacillus paracasei subsp. paracasei is deposited in the China General Microbiological Culture Collection Center andhas deposit number of CGMCC NO.17893. The lactobacillus paracasei subsp. paracasei has certain antioxidant capacity, can remove DPPH and hydroxyl radicals, also has good salt resistance, acid resistance, bile salt resistance, gastric juice resistance, intestinal juice resistance, and hydrophobicity, thus increases chances of playing a probiotic role, and can be used in fields of fermented feeds, fermented food, health-care food, etc.

The invention relates to pure natural lactobacillus millet enzyme. The pure natural lactobacillus millet enzyme is prepared from the following steps: preparing powder: smashing millet, and screening by a 100-mesh sieve; carrying out saccharification liquid preparation: weighing 200g of obtained millet powder, adding 1000mL of water, heating to 50 DEG C, adding glucoamylase for saccharification, heating to 85DEG C after saccharification is carried out, and maintaining for 10 minutes; blending and inoculating: weighing 1000g of obtained millet powder, adding 4000mL of water, adding saccharification liquid of which the water volume is 10-20%, and inoculating lactobacillus fermentium, lactobacillus reuteri, lactobacillus plantarum and bifidobacterium adolescentis at the ratio of 3:1:2:1; fermentation: sealing a fermentation container, carrying out fermentation for 90 days under the condition of 28-35 DEG C, and carrying out after-ripening fermentation for 30 days under the condition of 4-10 DEG C, thus obtaining the pure natural lactobacillus millet enzyme after fermentation ends. No food additive is added into the pure natural lactobacillus millet enzyme, and the pure nature and the safety of food can be guaranteed furthest.

The invention belongs to the field of microecological preparations containing Chinese herbal medicines, and discloses forage clostridium butyricum, a microecological preparation with a function of promoting livestock and poultry growth and a preparation method of the microecological preparation. The preservation number of the clostridium butyricum RCM-2 is CCTCC NO: M2017396. A microecological preparation product prepared from the clostridium butyricum RCM-2 through fermentation comprises substrates in percentage by mass as follows: 25%-30% of powdery radix pseudostellariae rootlet extract, 15%-20% of fructus crataegi, 15%-20% of pericarpium citri reticulatae, 15%-20% of medicated leaven, 10%-15% of folium perillae, 5%-10% of chicory root, 10%-20% of green tea and 0.05%-0.1% of allicin. The clostridium butyricum RCM-2 has good resistance to tea polyphenol and allicin, and besides, the powdery radix pseudostellariae rootlet extract can remarkably promote growth of the strain. The microecological preparation not only is beneficial to preservation of effective constituents of a finished product, but also can obviously reduce damage rate of goods and prolong shelf time.

The invention belongs to the technical field of fermentation of probiotics, and relates to a solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention discloses a natural astaxanthin-containing premix compound which comprises the following components according to weight ratio: 30-80g / kg of broken wall haematococcus pluvialis powder, 150-300g / kg of bean pulp, 150-200g / kg of calcium hydrophosphate, 150-250g / kg of mountain flour, 50-70g / kg of table salt, 0.6-0.9g / kg of nicotinic acid, 0.1-0.2g / kg of D-calcium pantothenate, 0.0016-0.0024g / kg of biotin, 7.5-12.5g / kg of choline chloride, 0.256-0.512g / kg of copper salt based on the content of copper, 1-2g / kg of ferric salt based on the content of ferrum, 1.2-2.4g / kg of zinc salt based on the content of zinc, 1.3-2.6g / kg of manganese salt based on the content of manganese, 0.02-0.04g / kg of iodate salt based on the content of iodate, 0.003-0.007g / kg of selenium salt based on the content of selenium, 20-35g / kg of methionine, 0.33-1.0g / kg of ethoxy quinoline, 1-3g / kg of phytase, 5-15g / kg of composite microecologics, 1-3g / kg of xylo-oligosaccharide and 80-120g / kg of bentonite. The natural astaxanthin-containing premix compound can remarkably improve the laying rate, reduce mortality and elimination rate, ensure proper colors of red-yolk eggs, promote the feed conversion rate, improve the production performance of laying hens and the eggshell quality, reduce the occurrence of diseases, and improve the economic efficiency.

The invention provides enterococcus faecium, a high-density fermentation method of enterococcus faecium, and a microecological preparation prepared by adopting enterococcus faecium, belonging to the technical field of culturing of lactic acid bacteria. An enterococcus faecium strain with the obvious probiotic property and stress resistance is screened and taken as a feed additive, and the enterococcus faecium strain can effectively replace part of antibiotics. The enterococcus faecium provided by the technical scheme is E13-5, and is assigned with the preservation number of CCTCC M2017191. Theenterococcus faecium provided by the invention can be used as the safe and efficient novel feed additive for replacing part of the antibiotics, and thus the demands of the modern feed industry and livestock breeding industry are satisfied.

The invention relates to a preparation technology of a bacillus feed additive agent, belonging to the field of feed additive agent. The preparation technology comprises the following steps: 1) culturing bacillus to obtain a seed liquid; 2) carrying out liquid fermentation culture of bacillus with the inoculation amount of the seed liquid is 3-10% and until the microbial content reaches 1-2*10<10>cfu / ml; 3) collecting thalli by centrifugation; 4) freeze-drying thalli: adding a freeze-drying protectant in the collected thalli, putting the mixture in a freeze dryer for vacuum freeze-drying to obtain freeze-dried bacterial powder; and 5) adding auxiliary materials in the freeze-dried bacterial powder to obtain the bacillus feed additive agent. The feed additive agent obtained by the preparation technology has good probiotic functions of stress resistance and preventing pathogenic bacteria such as gastric acid resistance, cholate resistance, high temperature resistance, antibiotic tolerance and the like, can be used in combination with antibiotics. The prepared feed additive has high viable bacteria content, long -term storage, and obvious and stable application effects.

The invention relates to a pediococcus acidilactici GQ01 derived from wolfberry enzyme and having uric acid reducing effect, the name of the pediococcus acidilactici is GQ01, the classification name of the pediococcus acidilactici is pediococcus acidilactici, the preservation number of the pediococcus acidilactici is CGMCC No.21609, the preservation date of the pediococcus acidilactici is January 13, 2021, and the preservation unit of the pediococcus acidilactici is China General Microbiological Culture Collection Center, which is located at Institute of Biology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The pediococcus acidilactici GQ01 disclosed by the invention is derived from the natural wolfberry enzyme, enriches the microbial composition in the fermented food, and has positive significance in promoting the development and industrial production of the fermented food such as the enzyme and the like. The pediococcus acidilactici GQ01 has better acid resistance and cholate resistance, has an obvious uric acid reducing effect, and can relieve side reactions such as inflammation caused by hyperuricemia.

The invention belongs to the technical field of fermentation of probiotics, and relates to a mixed solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus plantarum HM-10, Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention provides an Enterococcus faecium R40 and its application in lowering cholesterol, producing exopolysaccharide and anti-oxidation. The Enterococcus faecium R40 has the functions of lowering cholesterol, producing extracellular polysaccharides and anti-oxidation, and is more suitable for growth under acidic conditions. It is more tolerant to gastrointestinal conditions and has relatively good bile salt tolerance. Live bacteria or metabolites of Enterococcus faecium R40 can be added to food to lower cholesterol, resist oxidation, and aid digestion in the human body. The present invention also includes the application of Enterococcus faecium R40 in yoghurt and the yoghurt containing Enterococcus faecium R40. The yoghurt is better than commercial starter fermented yoghurt in every index.

The invention belongs to the technical field of fermentation of probiotics, and relates to a solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention proposes an Enterococcus faecium, its high-density fermentation method and a microecological preparation prepared therefrom, which belong to the technical field of lactic acid bacteria cultivation. Enterococcus faecium strains with significant probiotic and stress resistance are screened out as feed additives, which can effectively Replace some antibiotics. The Enterococcus faecium provided by the technical solution is E13-5, and the preservation number is CCTCC M2017191. The enterococcus faecium provided by the invention can be used as a safe and efficient new feed additive to partially replace antibiotics, thereby meeting the requirements of the modern feed industry and animal husbandry.

The present invention relates to a Pediococcus lactis GQ01 derived from Lycium barbarum enzyme with the effect of reducing uric acid. The name of the Pediococcus lactis is GQ01, the classification name is Pediococcus acidilacticii, and the preservation number is: CGMCC No.21609. Date: January 13, 2021. The depository unit is: General Microbiology Center of China Committee for the Collection of Microbial Cultures, Institute of Biology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The Pediococcus lactis GQ01 of the present invention is derived from natural wolfberry enzymes, enriches the composition of microorganisms in fermented foods, and has positive significance for promoting the development and industrial production of fermented foods such as enzymes. Pediococcus lactis GQ01 has good acid resistance and bile salt resistance, and it has obvious uric acid-lowering effect, which can relieve inflammation and other side effects caused by hyperuricemia.

The invention provides pediococcus acidilactici separated from Tibetan kefir and application of the pediococcus acidilactici in prevention and treatment of rotavirus infection, the pediococcus acidilactici separated from the Tibetan kefir is named as pediococcus acidilactici Wang, the pediococcus acidilactici is preserved in the China Center for Type Culture Collection on October 28, 2021, and the preservation number is CCTCC NO: M 20211331. The pediococcus acidilactici is resistant to acid and cholate, has a good inhibiting effect on most pathogenic bacteria, is sensitive to antibiotics, and shows an excellent rotavirus resisting effect in in-vivo and in-vitro experiments.

The invention relates to a probiotic preparation containing Lactobacillus plantarum and Bacillus licheniformis and a preparation method thereof, belonging to the technical field of fermentation of probiotics. The invention particularly relates to a mixed solid state fermentation technique which uses bean pulp, bran and corn flour as matrixes and the probiotics Lactobacillus plantarum HM-10 and Bacillus licheniformis BL-09 as strains. The technique can be widely used in the aspects of animal microecology and the like. The method can be used for producing fermented feeds with special acid aroma; the viable count of the fermented product is high; and the degradation rate of the bean pulp protein is high. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention discloses a Lactobacillus reuteri (Lactobacillus reuteri CCTCC M20191116), which can significantly prevent the occurrence of tumors and significantly prolong the survival of tumor-bearing mice by detecting its function of antagonizing tumor cells through cell experiments and animal models time. Tumor-bearing mice fed with L. reuteri CCTCC M20191116 can maintain normal alanine aminotransferase levels, indicating that L. reuteri CCTCC M20191116 can prevent melanoma cells from injuring the liver. The invention belongs to the technical field of microorganisms. The provided L. reuteri CCTCC M20191116 can stimulate the body to produce anti-cancer cytokines, inhibit the migration and colonization of tumor cells, protect internal organs, and have the functions of preventing tumor occurrence and prolonging survival time.

Belonging to the premix field, the invention relates to an additive premix for laying hens. The premix of the invention mainly contains: soybean meal, calcium hydrogen phosphate, stone powder, salt, compound vitamin, nicotinic acid, D-calcium pantothenate, biotin, choline chloride, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, potassium iodide premix, sodium selenite premix, ethoxy quinoline, phytase, microecologics, xylo oligosaccharide, bentonite and rice husk. With xylo oligosaccharide and a plurality of probiotics and without antibiotic, the premix in the invention hasa reasonable formula and high viable bacteria content. Animal feeding experiments prove that the additive premix in the invention has ideal feeding effects. The ammonia concentration, the death and culling rate, and the broken egg rate can be reduced, while the laying rate and egg weight are enhanced. The composite premix provided in the invention can well promote the laying performance and health status of laying hens, and meet the needs of mass raising households, and is suitable for green and healthy culture so as to produce pollution-free egg products.

The invention belongs to the technical field of fermentation of probiotics, and relates to a mixed solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus plantarum HM-10, Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.