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Forage clostridium butyricum, microecological preparation with function of promoting livestock and poultry growth and preparation method of microecological preparation

A micro-ecological preparation, the technology of Clostridium butyricum, is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., which can solve the problem of tea fermentation probiotic strains lacking tea polyphenols, unable to achieve application effects, and excessive tea. Phenol oxidation loss and other problems, to achieve the effect of enhancing immunity, improving body immunity, and maintaining digestive function

Active Publication Date: 2017-09-01
FUJIAN BRADY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of highly efficient fermented tea probiotics on the market. The main reason is the lack of tea-fermented probiotic strains that can tolerate tea polyphenols. The dissolution rate is small, the oxidation loss of tea polyphenols is serious, and the ideal application effect cannot be achieved
In addition, most of the current fermented microecological preparations (water content 10-40%) are contaminated by mold during the shelf life, how to solve this problem is of great significance to improve the shelf life of finished products

Method used

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  • Forage clostridium butyricum, microecological preparation with function of promoting livestock and poultry growth and preparation method of microecological preparation
  • Forage clostridium butyricum, microecological preparation with function of promoting livestock and poultry growth and preparation method of microecological preparation
  • Forage clostridium butyricum, microecological preparation with function of promoting livestock and poultry growth and preparation method of microecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1--Isolation and identification of Clostridium butyricum RCM-2

[0074] 1. Isolation, enrichment and purification of Clostridium butyricum RCM-2

[0075] Under aseptic operation conditions, farmers in Xia Village, Zherong County, Ningde City, Fujian Province (coordinates: 119 。 52′04.1″E, 27 。 13′32.7″N) The cecal mucosal segment of a healthy adult rooster bred in a closed mountain forest, the mucosal mucus was scraped with a sterilized glass slide and placed in an EP tube, diluted with sterile normal saline for 10 2 Then heated to 60°C for 30min, took 0.1mL and spread it on the RCM agar plate containing bromocresol purple indicator (0.01%, g / mL), after coating, put the RCM agar plate in the anaerobic incubator , cultured at 37°C for 48 hours, selected the colonies that turned yellow in the medium, and purified them by streaking, and selected the rod-shaped strains that were positive for Gram staining and had spores in the microscopic examination, and conta...

Embodiment 2

[0086] Embodiment 2--clostridium butyricum RCM-2 anaerobic and aerobic fermentation in MRS liquid medium

[0087](1) Activation of Clostridium butyricum RCM-2: Wash the slant of the Clostridium butyricum RCM-2 test tube with sterile saline in a sterile environment, wash off the bacterial lawn on the surface of the medium, and then inoculate it into the MRS liquid In the culture medium, culture at 37°C for 48 hours;

[0088] (2) Anaerobic static fermentation of Clostridium butyricum RCM-2 in MRS liquid medium:

[0089] Inoculate the activation solution of Clostridium butyricum RCM-2 obtained in step (1) into an anaerobic serum bottle containing MRS liquid medium in an anaerobic box according to the inoculation amount of 3% (volume ratio), and incubate statically at 37°C for 48 hours. At the end of the culture, the number of live bacteria, spore rate and pH value of the fermentation broth were measured;

[0090] (3) Aerobic fermentation of Clostridium butyricum RCM-2 in MRS li...

Embodiment 3

[0094] Embodiment 3--The effect of Pseudostellaria ginseng extract on the growth and pH of Clostridium butyricum RCM-2

[0095] (1) Activation of Clostridium butyricum RCM-2: Same as step (1) in Example 2;

[0096] (2) Inoculate the activation solution of Clostridium butyricum RCM-2 obtained in step (1) into an anaerobic serum bottle containing MRS liquid medium in an anaerobic box according to the inoculation amount of 3% (volume ratio). The gradient of Radix Pseudostellariae Pseudostellaria extract in the MRS liquid medium in the serum bottle was successively set to (g / mL): 0%, 0.10%, 0.25%, 0.5%, cultured at 37°C for 48 hours, and the fermentation broth was measured at the end of the culture pH value and number of viable bacteria, the results are shown in Table 3;

[0097]

[0098] (3) Put the activation solution of Clostridium butyricum RCM-2 obtained in step (1) into a 500 mL Erlenmeyer flask filled with 100 mL of MRS liquid medium according to the inoculation amount ...

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Abstract

The invention belongs to the field of microecological preparations containing Chinese herbal medicines, and discloses forage clostridium butyricum, a microecological preparation with a function of promoting livestock and poultry growth and a preparation method of the microecological preparation. The preservation number of the clostridium butyricum RCM-2 is CCTCC NO: M2017396. A microecological preparation product prepared from the clostridium butyricum RCM-2 through fermentation comprises substrates in percentage by mass as follows: 25%-30% of powdery radix pseudostellariae rootlet extract, 15%-20% of fructus crataegi, 15%-20% of pericarpium citri reticulatae, 15%-20% of medicated leaven, 10%-15% of folium perillae, 5%-10% of chicory root, 10%-20% of green tea and 0.05%-0.1% of allicin. The clostridium butyricum RCM-2 has good resistance to tea polyphenol and allicin, and besides, the powdery radix pseudostellariae rootlet extract can remarkably promote growth of the strain. The microecological preparation not only is beneficial to preservation of effective constituents of a finished product, but also can obviously reduce damage rate of goods and prolong shelf time.

Description

technical field [0001] The invention belongs to the field of microecological preparations of Chinese herbal medicines, and in particular relates to a microecological preparation for feeding Clostridium butyricum and promoting the growth of livestock and poultry and a preparation method thereof. Background technique [0002] In the past, people paid attention to eating well, but now people not only pay attention to eating well, but more importantly, eat nutritious, healthy, safe, and reassuring. Therefore, "food is the first thing for the people, and safety is the first priority for food." To ensure the safety of animal-derived food, the prohibition of feeding antibiotics is an inevitable trend. Nutritious, healthy and safe meat, egg and milk foods are becoming more and more popular on the table. However, in the whole process of livestock and poultry breeding, antibiotics are still added to feed for a long time to promote growth and antibiotics are used indiscriminately for d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/18A23K10/30C12R1/145
CPCA23K10/12A23K10/18A23K10/30C12N1/20C12N1/205C12R2001/145Y02P60/87
Inventor 潘慧青张炎达肖建设杨荣平赵齐林贺桂
Owner FUJIAN BRADY PHARMA CO LTD
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