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Method for preparing three-layer embedded bifidobacterium microcapsules

A technology of bifidobacteria and three-layer embedding is applied in the field of preparation of three-layer-embedded bifidobacteria microcapsules, which can solve the problems of decreased viable bacteria, poor preservation, application limitations, etc., and achieves extended storage period and less leakage. , the effect of promoting proliferation

Inactive Publication Date: 2013-03-13
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because bifidobacteria are extremely sensitive to oxygen and have poor resistance to low pH values ​​(such as gastric acid), after oral consumption, the number of bacteria that can enter the intestinal tract alive is small, and its products generally have preservation. Poor performance, the number of viable bacteria decreases with the extension of storage time, which limits the application of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of three-layer embedded bifidobacterium microcapsules comprises the following steps:

[0031] (1) Inoculate 0.4g of Bifidobacterium freeze-dried bacteria powder (Bifidobacterium bifidum) into 18mL MRS liquid medium (purchased from Qingdao High-tech Park Haibo Biotechnology Co., Ltd.), and add Zn Medium volume 0.05% cysteine ​​hydrochloride, use this MRS liquid medium to activate three times under the culture condition of 37°C, transfer with 5% inoculum each time, and the first two times are under the culture condition of 37°C Cultivate under low temperature for 20-24 hours, cultivate for the third time at 37°C for 18 hours, then centrifuge (4000r, 15min) to discard the supernatant to obtain the bacteria sludge, wash the bacteria sludge twice and mix with 0.85-0.9% sterile physiological The brine was mixed to make a concentration of 1.0×10 11 wxya -1 Bacterial suspensions are ready for use.

[0032] (2) Prepare a sodium alginate solution with a ...

Embodiment 2

[0040] The preparation method of three-layer embedded bifidobacterium microcapsules comprises the following steps:

[0041](1) Inoculate 0.4g of Bifidobacterium freeze-dried bacteria powder (Bifidobacterium longum) into 18mL of MRS liquid medium (purchased from Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.), and add Zn to the MRS liquid medium for culture Cysteine ​​hydrochloride with a base volume of 0.05% was used to activate the MRS liquid medium three times under the culture condition of 37°C, and the inoculation amount of 5% was transferred each time, and the first two times were under the culture condition of 37°C Cultivate for 20-24 hours, cultivate for the third time at 37°C for 18 hours, then centrifuge (4000r, 15min) to discard the supernatant to obtain the bacteria sludge, wash the bacteria sludge twice and mix with 0.85-0.9% sterile saline Mix and prepare to a concentration of 5.0×10 11 wxya -1 Bacterial suspensions are ready for use.

[0042] (2) Prepare a...

Embodiment 3

[0050] The preparation method of three-layer embedded bifidobacterium microcapsules comprises the following steps:

[0051] (1) Inoculate 0.4g of Bifidobacterium freeze-dried bacteria powder (Bifidobacterium lactis) into 18mL MRS liquid medium (purchased from Qingdao Hi-Tech Park Haibo Biotechnology Co., Ltd.), and add Zingiol to the MRS liquid medium for culture Cysteine ​​hydrochloride with a base volume of 0.05% was used to activate the MRS liquid medium three times under the culture condition of 37°C, and the inoculation amount of 5% was transferred each time, and the first two times were under the culture condition of 37°C Cultivate for 20-24 hours, cultivate for the third time at 37°C for 18 hours, then centrifuge (4000r, 15min) to discard the supernatant to obtain the bacteria sludge, wash the bacteria sludge twice and mix with 0.85-0.9% sterile saline Mix and prepare to a concentration of 1.0×10 10 wxya -1 Bacterial suspensions are ready for use.

[0052] (2) Prepar...

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Abstract

The invention discloses a method for preparing three-layer embedded bifidobacterium microcapsules, and the method comprises the following steps of: squeezing bifidobacterium glue solution into soybean oil containing an emulsifier by a pressure nozzle, thoroughly stirring, emulsifying, and then adding a calcium chloride solution and standing; after layering through standing, pouring the supernatant soybean oil out and centrifuging to obtain wet capsules, and then washing the wet capsules thoroughly by using normal saline, thereby obtaining the bifidobacterium microcapsules; mixing the bifidobacterium microcapsules with a two-layer embedding solution, oscillating and stirring, centrifuging, and then collecting the two-layer embedded bifidobacterium microcapsules; adding Tween-80 to palm oil, mixing evenly and thoroughly, and sterilizing, thereby obtaining a three-layer embedding solution; mixing the two-layer embedded bifidobacterium microcapsules with the three-layer embedding solution, oscillating and stirring, centrifuging and then collecting the three-layer embedded bifidobacterium microcapsules. The bifidobacterium is protected by the three-layer embedded microcapsule technology; and as a result, the storage periods of the bifidobacterium preparation and the product containing the bifidobacterium can be prolonged.

Description

technical field [0001] The invention belongs to the technical field of probiotic beverages, and relates to a preparation method of three-layer embedded bifidobacteria microcapsules. Background technique [0002] In recent years, with people's gradual understanding of the health care effects of probiotics, the research on their live bacteria preparations is also increasing. Bifidobacteria are a class of probiotics that can colonize the human intestinal tract and have important nutritional and health effects on the human body. However, because bifidobacteria are extremely sensitive to oxygen and have poor resistance to low pH values ​​(such as gastric acid), after oral consumption, the number of bacteria that can enter the intestinal tract alive is small, and its products generally have preservation. The disadvantages such as poor performance and the decrease of the number of viable bacteria with the prolongation of storage time limit the application of the product. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09A23P1/04B01J13/02
Inventor 陈合王野舒国伟
Owner SHAANXI UNIV OF SCI & TECH
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