Additive premix for laying hens
A premix and additive technology, applied in the fields of application, animal feed, animal feed, etc., can solve the problem of the non-genus of Phycocystis spp., instability of gastric acid and bile salt, no research and application reports, etc. problem, to achieve the effect of good eggshell color, reduced egg breaking rate and high egg production
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Embodiment 1
[0023] The preparation of embodiment 1 compound probiotics
[0024] 1. Preparation of Bacillus licheniformis CGMCC No.5094 bacterial powder
[0025] 1) Plate culture rejuvenation: Inoculate the Bacillus licheniformis strain on the BPY plate medium, culture at 37°C for 24 hours, rejuvenate the Bacillus licheniformis, and form a single colony, pick a single colony and inoculate it on the slant medium, Incubate at 37°C for 36 hours;
[0026] 2) Preparation of first-class seeds: transfer the Bacillus subtilis cultured in step 1) to the eggplant bottle slant medium, and cultivate them at 37° C. for 12 hours to make them in the mid-late logarithmic period to obtain first-class seeds;
[0027] Described slant medium is BPY solid medium;
[0028] 3) Preparation of secondary seeds: make bacterial suspension from the primary seeds prepared in step 2) with sterile water, and inoculate into 1.6M 3 2M of BPY seed medium 3 In the seed tank, the temperature is 37°C, the rotation speed is...
Embodiment 2
[0051] The preparation of embodiment 2 premix of the present invention
[0052] 1. The formula of multivitamin premix is as follows: vitamin A 30g, vitamin D3 10g, vitamin E 100g, vitamin K3 4g, vitamin B1 2g, vitamin B2 12g, vitamin B6 4g, vitamin B12 5g, niacin 90g, D-pantothenic acid Calcium 10g, biotin 8g, ethoxyquinoline 1g, rice husk bran (carrier) 724g, mixed to make multivitamin premix.
[0053] 2. The formula of the compound trace element premix is as follows: copper sulfate 25g, ferrous sulfate 150g, zinc sulfate 75g, manganese sulfate 198g, potassium iodide premix 100g, sodium selenite premix 8g, bentonite 440g, mixed to make Composite trace element premix.
[0054] 3, the preparation of premix of the present invention
[0055] The formula is as follows: 10g of compound vitamin premix prepared in step 1, 40g of compound trace element premix prepared in step 2, 5g of compound microecological preparation prepared in Example 1, 15g of choline chloride, 1g of xylo...
Embodiment 3
[0056] The preparation of embodiment 3 premixes of the present invention
[0057] 1. The formula of multivitamin premix is as follows: vitamin A 40g, vitamin D3 15g, vitamin E 50g, vitamin K3 4g, vitamin B1 6g, vitamin B2 8g, vitamin B6 8g, vitamin B12 9g, niacin 60g, D-pantothenic acid Calcium 20g, biotin 12g, ethoxyquinoline 1g, rice husk bran (carrier) 767g, mixed to make multivitamin premix.
[0058] 2. The formula of the compound trace element premix is as follows: copper sulfate 50g, ferrous sulfate 75g, zinc sulfate 148g, manganese sulfate 100g, potassium iodide premix 50g, sodium selenite premix 17g, bentonite 560g, mixed to make Composite trace element premix.
[0059] 3, the preparation of premix of the present invention
[0060] The formula is as follows: 10g of the compound vitamin premix prepared in step 1, 40g of the compound trace element premix prepared in step 2, 15g of the compound micro-ecological preparation prepared in Example 1, 25g of choline chlor...
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