At first, to proceed culturing activition to galotinous sporobacillus by one to three stages fermenting culture on substratum, bacterial liquid is adsorbed by danty powder when stirring to be galotinous sporobacillus bacterial agent. Then to proceed culturing activition to streptomycete strain by one to three stages fermenting culture on substratum, fermented liquid is adsorbed by danty powder when stirring to be streptomycete bacterial agent. Finally, danty, bone meal, phosphamide, urea, potassium sulfate, zinc sulfate, borax, magnesium sulfate, manganese sulfate, copper sulfate, ferrous sulfate, molybdate, galotinous sporobacillus bacterial agent and streptomycete bacterial agent are compounded to obtain the product in proper ratio. In the invention, biological engineering technology is used to produce double-effect composed microbial fertilizer, microbial bacterins with functions of depotassium, dephosphorus, nitrogen fixation and multipathogene fungus controlling are prepared to baterial agent by biological fermenting, high grade organic matter is used as carrier.