The invention discloses a preparation method of passion fruit wine, and belongs to the field of food processing. The preparation method comprises the steps of pulping, enzymolysis, yeast activation, primary fermentation, post fermentation, purification and the like. The preparation method of the passion fruit wine comprises the following steps of treating pulp and peel of passion fruit in a separated way; and performing enzymolysis on the peel of the passion fruit by using compound pectinase so that most tannin in the peel can be removed, the influence of bitter substances in the peel of the passion fruit on the fruit wine is avoided, and the peel of the passion fruit is changed from waste materials into valuable materials. The made passion fruit wine is clear; a wine body is clear and elegant; the intense passion fruit aroma is realized; the nutrition is rich; the mouthfeel is fragrant; the color is clear and bright; the efficacies of removing heat from the liver, eliminating toxic materials, invigorating the stomach to promote digestion, maintaining beauty, keeping young and improving the human body immunity are realized; and after the passion fruit wine is drunk by women for a long time, the effects of removing beverages, nourishing the skin, relieving ozostomia and promoting sleeping can be achieved. A wine making process is simple and feasible, is suitable for industrialized production, and has wide market prospects; and a novel field is opened for the sufficient utilization of passion fruit resources in China.