The present invention relates to a PCR detection
system, a kit and a detection method for detecting
vibrio parahaemolyticus. The PCR detection
system comprises a first primer pair and a first detection probe for detecting tlh
gene, a second primer pair and a second detection probe for detecting tdh
gene, and a third primer pair and a third detection probe for detecting ureR
gene. The PCR detectionsystem for detecting the
vibrio parahaemolyticus is based on simultaneous detection of three genes of the tlh, tdh and ureR, and can accurately distinguish specific types of the
vibrio parahaemolyticus. The PCR detection
system has good compatibility, very high specificity and sensitivity, and a
detection limit of 15 copies / ml. At the same time, the PCR detection system can directly use cells asa template for detection, and a detection effect is equivalent to that of using
DNA as a template. Therefore, a one-
step method from samples to detection can be achieved without
DNA extraction of thesamples.