The invention relates to a
reverse dot blot (RDB) detection membrane strip, a detection method and a kit for identifying insects. The membrane strip comprises a universal primer, a
solid phase carrier, a probe and a PCR positive
quality control plasmid, wherein the probe is fixed on the carrier; the universal primer is a
degenerate primer pair TF / TR; the
solid phase carrier is a Biodyne C membrane; the probe fixed on the carrier is a group of arrays consisting of a
quality control probe and an RDB detection probe; the
quality control probe comprises a color development point probe, a positive comparison probe and a negative comparison probe; and the RDB detection probe is a section of 15 to 19nt
oligonucleotide of which the 5' end is marked with amino (-NH2). The membrane strip can rapidly, correctly and vividly realize correct classified identification of adults, larvae, eggs, pupae and the like of insects and solves the problems of the need of professional classification knowledge, type specimen comparison, a large number of
insect types and difficult identification existing in the conventional morphological
insect identification, particularly the problem that the larvae, the eggs and the pupae of the insects and the adults of which the identification characteristics are damaged cannot be identified effectively.