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Fruit-fly classified detection biochip, detection method and reagent kit

A classification detection, biochip technology, applied in biochemical equipment and methods, microbial determination/inspection, material excitation analysis, etc. The effect of accurate classification identification and high-throughput identification

Inactive Publication Date: 2007-07-11
SHENZHEN AUDAQUE DATA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the Asia-Pacific region, there are as many as 75 known compound species of Bactrocera dorsalis, of which 8 are economically important, and the more common 5 species include B. (B.) dorsalis and its four important compound species (B. (B.) papayae, B. (B.) carambolae, B. (B.) philippinensis, B. (B.) occipitalis) complex species morphology The difference is very small, and it is not easy to distinguish. The classification and identification of Bactrocera dorsalis complex species has always been one of the difficulties of Tephritidae pests. The opinions of experts at home and abroad have not yet reached a complete agreement.
[0006] At present, most of the identification methods of fruit flies adopt traditional morphological identification, mainly through the morphological characteristics of adults to identify species, but cannot identify larvae and eggs, and there are errors in the identification of related species
However, in the port quarantine, the larvae and pupae are mainly intercepted. The characteristics of the larvae and pupae of Bactrocera are very similar, and it is difficult to identify the species. The traditional method is to raise the larvae or pupae to adults and then identify the species. , this process takes more than 20 days and is not suitable for the working procedures of rapid inspection and release of port quarantine

Method used

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  • Fruit-fly classified detection biochip, detection method and reagent kit
  • Fruit-fly classified detection biochip, detection method and reagent kit
  • Fruit-fly classified detection biochip, detection method and reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) 32 kinds of dangerous fruit fly classification and detection biochip

[0046] A biochip for the classification and detection of 32 dangerous fruit flies such as Mediterranean fruit fly and Bactrocera dorsalis, which is fixed with an oligonucleotide probe dot matrix on the surface of a solid carrier medium. Each substrate includes four identical probe dot matrix areas, and each probe dot matrix area includes 15 rows and 16 columns of dot matrix. Each probe array is divided into four functional probe areas, namely Anastrepha detection probe area, Bactrocera detection probe area, Ceratitis detection probe area and quality control probe area. Needle detection area (quality control probe is composed of positive control probe, negative control probe and blank control probe). The first row and the first column of the probe dot matrix are composed of positioning point probes. The dot matrix distribution of detection probes and quality control probes is shown in Table 3 and Figu...

Embodiment 2

[0123] Example 2 Biochip for classification and detection of five complex species of Bactrocera dorsalis and its detection method

[0124]Bactrocera dorsalis B. (B.) dorsalis and its four important compound species (Bactrocera dorsalis B. (B.) papayae, carambola fruit fly B. (B.) carambolae, Bactrocera philippines B. (B.) philippinensis, B. occipitalis) biochip detection probe arrangement is shown in Table 8 and Figure 2. Probes include 1 family universal probe, 1 genus universal probe, 1 subgenus universal probe, 6 compound species-specific probes, 2 species-specific probes, and a set of quality control probes (positive, negative) And blank control probe) and positioning point probe. The probe dot matrix is ​​shown in Figure 2, and each probe of the detection probe and the quality control probe is repeated 3 points horizontally. Refer to Table 1 and Table 2 for probe sequences. The above-mentioned probe can realize the preliminary screening of Bactrocera spp. species and distingu...

Embodiment 3

[0138] Example 3 Biochip for classification and detection of Mediterranean fruit fly and its related species and its detection method

[0139] The Mediterranean fruit fly C. capitata and its two related species: C. rosa natal and C. cosary biochip detection probe arrangement are shown in Table 9. In addition to the positioning point probe, the probe set also includes 1 family general probe, 1 genus general probe, 2 relative species-specific probes, 4 species-specific probes and a set of quality control (positive, negative) And blank control) probe. The probe dot matrix of the detection probe, the quality control probe and the positioning point probe is shown in Fig. 3, and each probe of the detection probe and the quality control probe is repeated 3 points horizontally. The above-mentioned probe set can realize the preliminary screening of Ceratitis spp. species and the identification of Mediterranean fruit flies, natal fruit flies and African fruit flies.

[0140] Table 9 Schemat...

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Abstract

The invention discloses a biological chip, testing method and testing agent box to classify Tephritidae, which selects probe of general-purpose probe and atopic probe or subgeneric general purposed probe and composite group / sibling species atopic probe to identify Tephritidae rapidly and precisely.

Description

Technical field [0001] The invention relates to insect classification and detection technology, in particular to a classification detection biochip, detection method and kit of Tephritidae. Background technique [0002] Ceratitis capitata and Bactrocera dorsalis are insects of the order Diptera and Tepheitida. These two kinds of insects are recognized as major agricultural pests in the world, and they are very widespread and mainly harm fruits and vegetables. [0003] Mediterranean fruit flies and orange fruit flies are respectively dangerous pests that are prohibited from entry by my country's entry plant quarantine. The Mediterranean fruit fly is one of the most destructive and dangerous pests to fruits and vegetables. It can harm all fruit crops that are valuable to humans (352 species have been reported), and are known as "the number one killer of fruits and vegetables." The Mediterranean fruit fly is native to West Africa and has now spread to more than 90 countries and regi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 余道坚章桂明李建光任鲁风杨伟东汪万春陈枝楠周琦徐浪
Owner SHENZHEN AUDAQUE DATA TECH
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