The invention discloses a biosecurity detecting, controlling and warning device and method, which comprises a shell forming air passage, at least a filter, an air inlet and an air outlet. The invention also comprises: at least a wind speed sensor, at least a pressure difference sensor, at least a wind speed transducer, at least a pressure difference transducer, a data processor, a monitor, a warner and a blower. The filter is a high-efficiency filter, the wind speed sensor is a hot-bulb wind speed sensor, the pressure difference sensor is sensistor micropressure difference sensor, the wind speed transducer is a hot-bulb balanced breeze speed transducer, the pressure difference is sensistor resistivity balanced micropressure transducer and the data processor is CPU or MPU data processor. The method can be used on the above device. The invention can accurately detect the failure, damage of the filter (HEPA or ULPA) and accurately alarm, which can also conduct automatic supplementing and controlling.