1098results about "Inorganic cation exchangers" patented technology

A combination of acid zirconium phosphate and alkaline hydrous zirconium oxide are utilized as ion-exchange materials, for example, in sorbent dialysis. The combination provides for dialysate regeneration while maintaining constant and controlled levels of Na+, HCO3−, and pH.

The present invention provides a positively charged microporous membrane having a protein binding capacity about 25 mg / ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

A method for adsorption of a substance from a liquid sample on a fluidized bead or stirred suspension, in which the beads used comprise a base matrix and exhibit a structure having affinity to the substance, characterized in that the structure is covalently bound to the base matrix via an extender. Populations of beads in which the beads contain a filler incorporated in a base matrix and an extender are also described.

PCT No. PCT / SE97 / 00237 Sec. 371 Date Dec. 29, 1998 Sec. 102(e) Date Dec. 29, 1998 PCT Filed Feb. 14, 1997 PCT Pub. No. WO97 / 29825 PCT Pub. Date Aug. 21, 1997Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, beta -lactoglobulin 1 and beta -lactoglobulin 2 on the anion exchanger is higher than the elution ionic strength required for a quaternary comparative ion exchanger.

The present invention relates to novel microporous zirconium silicate compositions that are formulated to remove toxins, e.g. potassium ions, from the gastrointestinal tract at an elevated rate without causing undesirable side effects. The preferred formulations are designed avoid increase in pH of urine in patients and / or avoid potential entry of particles into the bloodstream of the patient. Also disclosed is a method for preparing high purity crystals of UZSi-9 exhibiting an enhanced level of potassium exchange capacity. These compositions are particularly useful in the therapeutic treatment of hyperkalemia.

Herein is disclosed a flue gas scrubbing composition, a method of using the flue gas scrubbing composition, and a method of entombing mercury collected with the flue gas scrubbing composition. The flue gas scrubbing composition includes an admixture of a mercury sorbent material that comprises a clay, copper, and sulfur; and lime that comprises calcium oxide and / or calcium hydroxide. The method of collecting mercury from a flue gas includes injecting the flue gas scrubbing composition of any one of the preceding claims into a flue duct comprising the flue gas; reacting the mercury sorbent material with mercury in the flue gas to form a mercury-sorbed material and thereby reducing the concentration of mercury in the flue gas; reacting the lime with SO2, SO3, and / or HCl in the flue gas to form a calcium sulfate and / or a calcium chloride; and collecting a mixture that includes the mercury-sorbed material. The method of entombing mercury includes mixing the collected mixture of any one of claims 6 to 9 with water to form a freshly mixed concrete; and casting the freshly mixed concrete into a form.

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

A combination of acid zirconium phosphate and alkaline hydrous zirconium oxide are utilized as ion-exchange materials, for example, in sorbent dialysis. The combination provides for dialysate regeneration while maintaining constant and controlled levels of Na+, HCO3−, and pH.

A blood based solute monitoring system for measuring at least one blood solute species that has a first recirculation flow path in fluid communication with a dialyzer. The first recirculation flow path is configured to allow a fluid to recirculate through a dialyzer such that the concentration of at least one solute species in the fluid becomes equilibrated to the solute species concentration of the blood in a blood compartment of the dialyzer. The blood solute monitoring system has at least one sensor to measure a fluid characteristic.

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg / ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.

A system for measuring at least one fluid characteristic at various stages within a sorbent system that has a sorbent cartridge that has at least one material layer and at least one fluid passageway in at least one location in the sorbent system to provide a diverted sample stream from the various stages. At least one fluid characteristic of the diverted sample stream is measured.

A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.

A method for purifying a polypeptide by ion exchange chromatography is described in which a gradient wash is used to resolve a polypeptide of interest from one or more contaminants.

A method for purifying a polypeptide by ion exchange chromatography is described in which a gradient wash is used to resolve a polypeptide of interest from one or more contaminants.

The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quaternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.

A method for making an ion exchange coating (e.g., a chromatographic medium) on a substrate comprising (a) reacting at least a first amine compound comprising amino groups, with at least a first polyfunctional compound, in the presence of a substrate to form a first condensation polymer reaction product, with a first unreacted excess of either at least said first amino group or polyfunctional compound functional moieties, irreversibly attached to the substrate, and (b) reacting at least a second amine compound or at least a second polyfunctional compound with unreacted excess in the first condensation polymer reaction product to form a second condensation polymer reaction product, and repeating the steps to produce the desired coating. A coated ion exchange substrate so made.

The invention relates to a system and a process for fractionating a solution into two or more fractions. The system of the invention comprises at least two compartments having a diameter of at least about one meter and including a uniform packing of a polymer-based ion exchange resin with a bead size in the range of about 50 to about 250 mum. The mixing volume of the fluid fronts in the system of the invention is not more than 5% of the volume of the compartment.

The present invention relates to a method of separating sugars and sugar alcohols from each other. More particularly the present invention relates to the use of a weakly basic anion exchange resin in a chromatographic separation process. The advantage of the present invention compared with the prior art is that it is especially suitable for separating reducing sugars in acidic conditions as well as for example in weakly acidic conditions. The method using chromatographic separation comprises at least one step where a weakly basic anion exchange resin is used in a chromatographic column or in a part of a column.

Abstract of the DisclosureA cationically charged material suitable for use as a filter medium that is capable of reducing a large number of active microorganisms from a fluid such as water. The filter medium includes a substrate material. Portions of the substrate material are modified by adhesion of a cationic polymer thereto, the cationic polymer having aluminum-containing groups. The cationically charged material can further include an antimicrobially active metal complexed with the polymer. Illustrative metals include copper, zinc, tin, and preferably silver.

The present invention relates to a method of isolating a target compound from other components of a liquid, which method comprises at least two chromatographic steps, in any sequence of order, wherein the mobile phase is contacted with an affinity chromatography matrix and / or an ion-exchange chromatography matrix and / or a hydrophobic interaction chromatography matrix, wherein the contacting with at least one of the matrices takes place in the presence of at least one non-ionic polyether; and obtaining the target compound(s) in a separate fraction from the last chromatographic step. In the most preferred embodiment, the non-ionic polyether is poly(ethylene glycol) (PEG).

The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.

The invention relates to chromatographic separating materials having improved binding capacity for biological constituents in cell culture supernatants, or animal or human body fluids, in particular for monoclonal antibodies. The present invention likewise relates to the preparation of separating materials of this type, and to the use thereof, in particular for the removal of charged biopolymers from corresponding liquids.

The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quaternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.

Binderless BaKX zeolitic adsorbents, methods for their production, and processes for their use in a liquid phase adsorptive separation process are provided. An adsorbent includes a binder-converted zeolite portion formed from x wt % kaolin clay binder and (100-x) wt % unconverted Zeolite X with a silica:alumina molar ratio of about 2.5. The kaolin clay binder is in the range of about 10 to about 20 wt %. Ba and K occupy cationic exchangeable sites within the adsorbent. K is in the range of about 0.25 to about 0.9% by weight and Ba is greater than about 31.6% by weight of the binderless BaKX zeolitic adsorbent. Cornstarch may be added to the Zeolite X and kaolin clay binder to increase adsorbent macro-porosity and pore volume. Productivity of the adsorbent is improved decreasing process operating costs. The mechanical strength of the adsorbent is also improved.

The present invention relates to novel microporous zirconium silicate compositions that are formulated to remove toxins, e.g. potassium ions, from the gastrointestinal tract at an elevated rate without causing undesirable side effects. The preferred formulations are designed avoid increase in pH of urine in patients and / or avoid potential entry of particles into the bloodstream of the patient. Also disclosed is a method for preparing high purity crystals of ZS-9 exhibiting an enhanced level of potassium exchange capacity. These compositions are particularly useful in the therapeutic treatment of hyperkalemia.

Methods and compositions for removing a contaminant from its environment. The method includes forming a magnetic composition comprising the contaminant and an amphiphilic substance, and applying a magnetic field to the magnetic composition so as to separate the magnetic composition from the environment. One composition includes a micelle array confined in a magnetic mesoporous framework. Another composition is formed by adhering an amphiphilic material comprising functional surface groups to a contaminant, then interacting a magnetic material with the functional surface groups of the amphiphilic material. In various versions, the contaminant can be a hydrophobic organic compound, or a fullerene-related nanoparticle. The methods can also be used to purify hydrophobic organic compounds or fullerene-related nanoparticles.