The invention discloses a test rat feeding box. The test rat feeding box consists of a feeding box body, combined multifunctional lamps, a ventilation system assembly, multimedia speakers, humidity regulators, rat nest devices, driven drums, a drinking water bottle assembly, temperature regulators, a stair, drum active driving component assemblies, a lower wire gauze, a feed groove, a feeder assembly, an upper wire gauge, a stair outlet, a roller and a main assembly controller. The feeding box structure is partitioned into an upper layer and a lower layer which are isolated through a separating plate; the stair outlet is formed in the separating plate; a feeding box body upper cover is arranged on the upper part of the feeding box body; a foldable side door is arranged on one side wall of the lower layer of the box body. The functions of automatic disinfection, excrement cleaning, internal box temperature and humidity regulation, observation of the behaviors or health degrees of test rats, performance of noise test, ventilation and air exchange, creation of a proper illumination environment and the like are integrated, and the automation, safety and convenience of the test rat feeding box are superior to those of the conventional test rat feeding box. The test rat feeding box is convenient to maintain and manage, and has high cost performance and a high market value.