The invention relates to a fish bait for an oxygen fish-gathering pit. The fish bait is prepared from, by weight, 7-20 parts of musk millet, 10-30 parts of corn grits soaked with baijiu, 15-30 parts of rice soaked with baijiu, 7-30 parts of corn flour, 12-30 parts of fine wheat germs, 15-30 parts of bentonite, 10-50 parts of rapid oxygen releasing powder, 3-10 parts of a binding agent, 0.3-5 parts of fruit essence and 1.33-3 parts of water. The fish bait for the oxygen fish-gathering pit has the advantages that the substance capable of releasing oxygen is added into the pit bait, and the fish gathering verification problem can be effectively solved; the fish bait can rapidly release the oxygen after being added into water, a fish luring substance capable of conducting up-and-down stereoscopic movement is provided, the bottom fish bait is reserved, and fish in the upper layer, fish in the middle layer and fish in the lower layer can be lured and gathered to enter the pit.