127 results about "Th polarization" patented technology

The invention discloses a method for efficiently and sequentially inducing macrophages to be polarized in vitro. The method comprises the following steps of: S1, carrying out multiplication culture onTHP-1 cells by using an RPMI1640 culture medium containing 10% of FBS and beta-mercaptoethanol; S2, carrying out pretreatment before induction on the THP-1 cells by using PBS containing HPL and a culture medium; S3, in an RPMI1640 culture medium which contains 5% of HPL and does not contain the beta-mercaptoethanol, slowly inducing the THP-1 cells by adopting low-concentration PMA so as to enablethe THP1 cells to be differentiated into M0 macrophages in an adherence mode, and then removing the PMA; and S4, in an RPMI1640 culture medium which contains 5% of HPL and does not contain the beta-mercaptoethanol, inducing M0 macrophages for more than 72 hours by using LPS/IFN-gamma, so that the M0 macrophages are polarized into M1; or inducing the M0 macrophages for more than 72 hours by usingIL-4/IL-13, so that the M0 macrophages are polarized into M2 macrophages. Compared with the prior art, the method disclosed by the invention has the advantages that firstly, the THP-1 cells are slowlyinduced by adopting the low-concentration PMA, so that the immatureness state of the M0 type macrophages differentiated from the THP1 cells can be guaranteed; and then the optimal induced polarization time of the subsequent M1/M2 type macrophages is over 72 hours, so that the polarization state of the M1/M2 type macrophages is guaranteed. Meanwhile, the differentiation process is carried out in an environment without animal-derived components, so that the polarization efficiency of the macrophages can be effectively ensured.