Nanoparticle coated with ferroferric oxide by macroporous organic silicon oxide and preparation method and application thereof

An organic silicon oxide and ferric oxide technology, which is applied in the field of nanomaterials and achieves the effects of low equipment requirements, favorable for large-scale production and uniform size

Inactive Publication Date: 2018-11-23
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, composite nanoparticles formed by combining Fe3O4 and mesoporous organosilica have not been used in the immunotherapy of tumors.

Method used

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  • Nanoparticle coated with ferroferric oxide by macroporous organic silicon oxide and preparation method and application thereof
  • Nanoparticle coated with ferroferric oxide by macroporous organic silicon oxide and preparation method and application thereof
  • Nanoparticle coated with ferroferric oxide by macroporous organic silicon oxide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Synthesize superparamagnetic Fe3O4 nanoparticles using the reported method, and then disperse 30 mg of the prepared Fe3O4 nanoparticles into a solution containing 0.16 g of surfactant, 30 mL of ethanol, and 1 mL of ammonia water and 75 mL of water, ultrasonically disperse for 5 min, and stir rapidly at 35°C for 0.5 h; the molar ratio of surfactant, ethanol, ammonia water and water is 1: 4: 5900: 37900: 264 , wherein the massfraction of ammoniacal liquor is 25%;

[0039] (2) Add 25mL cyclohexane to the mixed liquid in step (1), and let it stand still for 10 minutes to form a water-ethanol / alkane two-phase reaction system;

[0040] (3) Add the silane precursor composed of tetraethyl orthosilicate and 1,2-bis(triethoxysilyl)ethane to the upper alkane phase in the two-phase reaction system obtained in step (2) , and reacted for 48 h under the condition that the reaction temperature was 20°C and the stirring speed was 200 rpm;

[0041] (4) Centrifuge at 8000 rpm, wash wi...

Embodiment 2

[0049] (1) Synthesize superparamagnetic Fe3O4 nanoparticles using the reported method, and then disperse 30 mg of the prepared Fe3O4 nanoparticles into a solution containing 0.16 g of surfactant, 30 mL of ethanol, and 1 mL of ammonia water and 75 mL of water, ultrasonically dispersed for 5 min, and stirred rapidly at 35°C for 0.5 h, the molar ratio of the surfactant, ethanol, ammonia and water was 1:4:5900:37900:264, Wherein the massfraction of ammoniacal liquor is 25%;

[0050] (2) Add 25 mL of n-hexane to the mixed liquid in step (1) and let it stand still for 10 min to form a water-ethanol / alkane two-phase reaction system;

[0051] (3) Add the silane precursor composed of tetraethyl orthosilicate and 1,2-bis(triethoxysilyl)ethane to the upper alkane phase in the two-phase reaction system obtained in step (2) , and reacted for 48 h at a reaction temperature of 60 °C and a stirring speed of 200 rpm.

[0052] (4) Centrifuge at 8000 rpm, wash with ethanol three times, dispers...

Embodiment 3

[0057] (1) Synthesize superparamagnetic Fe3O4 nanoparticles using the reported method, and then disperse 30 mg of Fe3O4 nanoparticles into a solution containing 0.3 g of surfactant, 30 mL of ethanol, and 1 mL of ammonia water and 75 mL of water, ultrasonically disperse for 5 min, and stir rapidly at 35°C for 0.5 h; the molar ratio of surfactant, ethanol, ammonia water and water is 1: 7.5: 5900: 37900: 264 , wherein the massfraction of ammoniacal liquor is 25%;

[0058] (2) Add 25 mL of n-hexane to the mixture in step (1), and let it stand for 10 minutes to form a water-ethanol / alkane two-phase reaction system;

[0059] (3) Add the silane precursor composed of tetraethyl orthosilicate and 1,2-bis(triethoxysilyl)ethane to the upper alkane phase in the two-phase reaction system obtained in step (2) , and reacted for 48 h under the condition that the reaction temperature was 20°C and the stirring speed was 100 rpm;

[0060] (4) Centrifuge at 8000 rpm, wash with ethanol three tim...

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Abstract

The invention discloses a nanoparticle coated with ferroferric oxide by macroporous organic silicon oxide and a preparation method and application thereof. An organic silicon oxide shell layer outsidethe nanoparticle has a large radial channel, and ferroferric oxide coated at an inner layer has superparamagnetism. The nanoparticle is synthesized in an oil-water two-phase system by using a surfactant as a template, and using organic and inorganic silane as a silicon source precursor under the action of oil regulation. The nanoparticle has a complete spherical structure, uniform size, and gooddispersibility, the nanoparticle with a macroporous structure is loaded with an antigen to be used for tumor immunotherapy, which not only improves the phagocytosis of dendritic cells (DCs) to the antigen, and can simultaneously stimulate the maturing of more antitumor T cells, thereby causing M1 type polarization of tumor associated macrophage, and showing an excellent anti-tumor effect, therefore a novel nano medical integrated platform for tumor immunotherapy is expected to be established.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a nanoparticle of macroporous organosilica-wrapped ferric oxide, a preparation method and an application thereof. Background technique [0002] Nanoparticles usually have huge specific surface area, high surface activity and ultra-small size, which make nanoparticles have unique structural characteristics and physical and chemical properties different from macroscopic substances. Traditional chemotherapy drugs combined with nanomaterials often have more High potency and low toxicity can produce more ideal curative effect. In recent years, the topic of using nanomaterials for tumor immunotherapy has attracted widespread attention from researchers. It has been reported that nanomaterials can activate immunity or overcome immunosuppression, thereby achieving anti-tumor effects; various nanomaterials are effective in antigen presentation. The use of nanoparti...

Claims

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Application Information

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IPC IPC(8): A61K49/08A61K39/00A61K41/00A61K47/69A61P35/00
CPCA61K39/0011A61K41/00A61K47/6949A61K49/08A61P35/00
Inventor 苏晓丹滕兆刚党萌陈昆马晓波
Owner NANJING UNIV OF POSTS & TELECOMM
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