The invention discloses a multi-
gene detection kit for anti-depression
drug medication guidance and a using method of the kit. A multi-PCR and fragment length /
mass analysis method is used for synchronously, quickly and quantitatively detecting seven
single nucleotide polymorphism (SNP) loci on four genes, including CYP2D6, CYP2C19, ABCB1 and
FKBP5, related to anti-depression
drug metabolism, transfer and
target site actions. Detection includes the steps that 1, oral cast-off cells are collected and stored in a
cell acquisition card or a blood sample is collected and
nucleic acid is extracted;2, the
cell acquisition card or the
nucleic acid in step 1 serves as a template for multi-PCR amplification; 3, according to the length /
mass of PCR product fragments, the seven SNP loci, three
human DNA referenc genes and a PCR product of a PCR reaction internal reference are synchronously separated; 4, results are subjected to analysis and interpretation. The kit has the advantages of being quickto use, high in sensitivity, good in
repeatability, high in specificity, high in flux and low in cost.