Multiple gene detection kit for guiding antipsychotic drugs and using method thereof

A technology for antipsychotic drugs and medication guidance, which is applied in biochemical equipment and methods, microbial determination/examination, recombinant DNA technology, etc. Positive, reproducible and specific effects

Inactive Publication Date: 2019-07-12
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Sanger sequencing method is complex and expensive
When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive
The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical ...

Method used

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  • Multiple gene detection kit for guiding antipsychotic drugs and using method thereof
  • Multiple gene detection kit for guiding antipsychotic drugs and using method thereof
  • Multiple gene detection kit for guiding antipsychotic drugs and using method thereof

Examples

Experimental program
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Embodiment 1

[0050] In this example, oral exfoliated cells of a patient with schizophrenia were collected, multiple PCR reactions were directly performed using the oral exfoliated cell collection card as a template, and finally the samples were separated by capillary electrophoresis. The specific steps are as follows:

[0051] 1. Production of multiple gene detection kits for antipsychotic medication guidance, including the following components:

[0052] 1) primer composition Sch Primer Mix;

[0053] 2) PCR reaction solution;

[0054] 3) Positive control substance Sch POS;

[0055] 4) Ultrapure water.

[0056] 2. Collection of samples

[0057] The oral exfoliated cells of a mentally ill patient were collected with a buccal swab and stored on a cell collection card.

[0058] 3. Use the cell collection card as a template for PCR reaction

[0059] 1) Add reagents and samples to the 96-well sample plate / PCR eight-tube tube according to Table 4.

[0060] Table 4 PCR reaction system

[00...

Embodiment 2

[0082] In this embodiment, a whole blood sample of a schizophrenia patient is collected and nucleic acid is extracted, and multiple PCR reactions are performed using the extracted nucleic acid as a template, and finally the sample is separated by capillary electrophoresis. The specific steps are as follows:

[0083] 1. Production of multiple gene detection kits for antipsychotic medication guidance, kit components as described in Example 1.

[0084] 2. Collection of samples

[0085] A whole blood sample was collected from a patient with schizophrenia and nucleic acid was extracted.

[0086] 3. Use the extracted nucleic acid as a template for PCR reaction

[0087] 1) Add reagents and samples to the 96-well sample plate / PCR eight-tube tube according to Table 9.

[0088] Table 9 PCR reaction system

[0089] Reagent Amount (μL) / well PCR reaction solution 14 Primer composition 2 nucleic acid 2 water 2 Total 20

[0090] 2) Mix and ce...

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Abstract

The invention discloses a multiple gene detection kit for guiding antipsychotic drugs and a using method thereof. The kit adopts multiple PCR combined with a fragment length/mass analysis method and synchronously and qualitatively detects 7 nucleotide polymorphism (SNP) locus on 6 genes including CYP2D6, MC4R, CYP1A2, CYP3A5, EPM2A, and SLC6A2 which are related to antipsychotic drug metabolism, transport and target action. The method comprises the detection steps of (1) collecting oral exfoliated cells and storing on a cell collection card or collecting blood samples and extracting nucleic acids; (2) performing multiple PCR amplification using the cell collection card or nucleic acid as described in step 1 as formwork; (3) according to PCR product fragment length/mass, simultaneously separating into 7 SNP loci, 3 human DNA internal reference genes and 1 PCR reaction internal reference PCR product; (4) analyzing results and interpreting. The kit has the advantages of fast performance, high sensitivity, good repeatability, strong specificity, high throughput and low cost.

Description

technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit for guiding the use of antipsychotic drugs and a method for using the same. Background technique [0002] With the rapid development of my country's social economy and the increasing pressure of competition, the incidence of mental illness is increasing year by year. The prevalence rate of mental illness in our country is about 17%. Schizophrenia is one of the most serious mental illnesses, affecting approximately 1% of the population worldwide. Schizophrenia has a variety of complex clinical manifestations, mainly characterized by changes in basic personality, incoordination between thinking, emotion, and behavior, and separation of mental activities from reality. Schizophrenia not only brings great pain to the patients themselves and their families, but also brings a heavy economic burden to the family, the medical system and the whole society. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/6883C12Q1/686C12Q2600/156C12Q2537/143
Inventor 吕军英陈真王凡吴勇余丁
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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