A minimally invasive surgical instrument for repairing mitral valve regurgitation by implanting artificial chordae through the apex, including sequentially connected clamps, catheters and flange joints, as well as opening and closing pull rods, locking push rods and puncture guide wires; the clamps are Round head clamps, including movable jaws, fixed jaws and fixed jaw base, the movable jaws are provided with curved guide holes for axial entry and radial exit, and the fixed jaws are provided with radial V-shaped through grooves; puncture The guide wire can pass through the flange joint, the catheter, the fixed jaw seat, the curved guide hole of the movable jaw, the radial V-shaped slot of the fixed jaw, and pass through the radial V-shaped slot of the fixed jaw ; The locking push rod sequentially passes through the flange joint, the conduit, and the fixed clamp seat to reach the radial V-shaped through groove of the fixed clamp. The device of the invention is small in size, compact in structure, simple in operation, greatly shortens operation time, improves operation efficiency and safety, has small operation trauma, is safe and convenient, and can achieve better operation effect.