33 results about "Starch binding" patented technology

Compositions and methods for manufacturing sheets having a starch-bound matrix reinforced with fibers and optionally including an inorganic mineral filler. Suitable mixtures for forming the sheets are prepared by mixing together water, unmodified and ungelatinized starch granules, an auxiliary water-dispersible organic polymer, fibers, and optionally an inorganic mineral filler in the correct proportions to form a sheet having desired properties. The mixtures are formed into sheets by passing them between one or more sets of heated rollers to form green sheets. The heated rollers cause the auxiliary polymer to form a skin on the outer surfaces of the sheet that prevents the starch granules from causing the sheet to adhere to the rollers upon gelation of the starch. The green sheets are passed between heated rollers to gelatinize the starch granules, and then to dry the sheet by removing a substantial portion of the water by evaporation. The starch and auxiliary polymer form the binding matrix of the sheets with the fibers and optional inorganic filler dispersed throughout the binding matrix. The starch-bound sheets can be cut, rolled, pressed, scored, perforated, folded, and glued to fashion articles from the sheets much like paper or paperboard. The sheets are particularly useful in the mass production of containers, such as food and beverage containers.

A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genus Rhizopus. The said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

A method of immobilizing a molecular species that include a starch-binding domain is provided. There also is provided a material upon which the molecular species is immobilized, and a material that is capable of immobilizing the species The method includes binding the species to a solid support, e.g., membranes, chromatographic supports and the like. The immobilized species is optionally purified by the method of the invention. Alternatively, the immobilized species is use in another method, such as in a synthesis as a synthetic reagent, or to purify another species that has an affinity for the immobilized species. Exemplary immobilized molecular species include bioactive agents, and biomolecules.

Polypeptides comprising a catalytic starch debranching domain and a starch binding domain are disclosed. The polypeptides comprising the starch binding domain have an improved functionality in raw starch degradation compared with same catalytic unit in same amount but without the starch binding domain.

The present invention relates to a method for preventing or treating virus and protozoa infection in a subject in need thereof comprising: orally administering to said subject an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species. The present invention also relates to a method for inducing interferon-gamma production in a subject in need thereof comprising: orally administering to said subject an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species. The present invention further relates to a method for improving feed intake, growth rate or feed conversion ratio in a subject in need thereof comprising: administering to said subject by oral administration or injection an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species.

The present invention relates to a starch binding domain, a recombinant protein and a complex thereof. The present invention also relates to a method for separating a recombinant protein comprising a starch binding domain of the present invention.

The present invention provides a method and a system for increasing thermal stability of a target protein comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix. The present invention also provides a method for retaining an activity of a target protein in aquatic environment comprising fusing a starch binding protein (SBP) with the target protein to form a SBP-tagged recombinant protein and combining the SBP-tagged recombinant protein with a SBP-binding matrix.

The invention discloses a preparation method of a highly branched dextrin product, which belongs to the field of high value utilization of starch deep processing transformation. The main steps are as follows: after heating the starch milk to gelatinize and decluster, use α-amylase to restrict hydrolysis, then treat with glycogen branching enzyme, and obtain a highly branched dextrin product through alcohol precipitation and drying. The present invention preliminarily utilizes α-amylase to decompose clustered starch, and combines the biocatalytic conversion technology of recombinant heterologously expressed glycogen branching enzyme of Actinomycetes Thermomonas genus Thermomonospora curvata to synergistically act on starch to convert long amylose The substrate is converted into a novel dextrin product with a highly branched structure. The invention significantly shortens the preparation time of highly branched dextrin products, greatly improves the product yield, and has the advantages of high branching, good solubility and the like.

The present invention provides a method and a system for protein purification using a starch binding protein (SBP)-tagged recombinant protein. SBP-binding matrixes are also disclosed in the invention to recover the SBP-tagged recombinant protein. Thus, purifying a target protein is re-usable, convenient and low cost by the present invention.

The present invention adopts citric acid as modifier, citric acid is combined with starch, plays a good role in modifying starch under high temperature conditions, improves the anti-digestion property of starch; It is very safe and has high practical value; the present invention adopts infrared treatment technology to make starch and citric acid undergo crosslinking reaction, and further dry heat treatment promotes the entry of citric acid modifier into starch, making the modification effect remarkable and stable, and the modification efficiency high.

The invention provides a high temperature resistant modified starch and its preparation method and application. The invention converts the crystalline region of the base starch into an amorphous region through the gelatinization process, so that the -OH at the C6 position of the starch structural unit is exposed to the solution environment In the process, the concentration of ‑OH that can participate in the reaction is increased, the grafting rate between the base starch and the grafting agent is increased, and then the temperature resistance is improved; and the initiator and modifier solution are added alternately to prolong the activity of the starch free radical Long time, increase the grafting rate of the reaction monomer, and then improve the temperature resistance effect of the modified starch; also use N, N-methylenebisacrylamide and tripropylene glycol dienyl ester as cross-linking agents, combined with starch, Form a three-dimensional network structure to improve the temperature resistance effect. The results of the examples show that the high temperature resistant modified starch provided by the present invention is suitable for both fresh water slurry and salt water slurry, and it can still maintain a high fluid loss after high temperature treatment at 150°C, and has excellent temperature resistance.

The present invention relates to a polysaccharide-protein binding model of SBD, and a fibril-forming 14-residue peptide consisting of X1NNNX2X3NYQX4X5X6X7X8, wherein the X1 and X8 mean a pair of opposite charged amino acid residues, and the X2, X3, X4, X5, X6, or X7 means an amino acid residue. The present invention also relates to a mixture for diminising a polysaccharide, comprising at least two starch binding domains (SBDs) and a polysaccharide in a helix form. The present invention further relates to a method of providing an oligosaccharide, and a method of producing an amyloid-like fibril and use thereof.

The present invention describes variants of alpha-amylases useful in industrial processes such as starch liquefaction. The α-amylase variants have enhanced specific activity, allowing for faster reduction of peak viscosity during liquefaction. The α-amylase is modified by introducing a mutation into the amino acid sequence of a parental Family 13 α-amylase polypeptide at an amino acid residue in the starch-binding groove; wherein the starch-binding groove is modified by the first β in the A domain Amino acid residues in the α-helix preceding the ‑strand, the loop between the sixth α‑helix and the seventh β‑strand in the A domain, the seventh α‑helix and the eighth β in the A domain - the loop between the chains, and the loop formation connecting the A domain and the C domain; and wherein the mutation changes the binding of starch to the variant alpha amylase polypeptide compared to the parent alpha amylase polypeptide.

The present invention relates to a method for preventing or treating virus and protozoa infection in a subject in need thereof comprising: orally administering to said subject an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species. The present invention also relates to a method for inducing interferon-gamma production in a subject in need thereof comprising: orally administering to said subject an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species. The present invention further relates to a method for improving feed intake, growth rate or feed conversion ratio in a subject in need thereof comprising: administering to said subject by oral administration or injection an effective amount of a composition comprising a complex consisting of a starch binding protein (SBP)-tagged immunostimulatory protein and a SBP-binding matrix, wherein the immunostimulatory protein is from Ganoderma species.

A polysaccharide-protein binding model of SBD, and a fibril-forming 14-residue peptide consisting of X1NNNX2X3NYQX4X5X6X7X8, wherein the X1 and X8 mean a pair of opposite charged amino acid residues, and the X2, X3, X4, X5, X6, or X7 means an amino acid residue is described. A mixture for diminising a polysaccharide, comprising at least two starch binding domains (SBDs) and a polysaccharide in a helix form is also presented. A method of providing an oligosaccharide, and a method of producing an amyloid-like fibril and use thereof are further described.