30 results about "Serum product" patented technology

A composition and method for immunization of mammals, containing antibodies from the eggs/egg yolks of chickens, or other suitable avian source, hyperimmunized against a selected pathogen(s) or immunogens, and a quantity of non-specific mammalian antibodies, such as those from commercial colostrum or serum products, which are combined to produce a composition that provides protection against the selected pathogen. Applications include viral, bacterial and parasitic infections.

The invention discloses a reovirus-detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) an RNA extraction solution, b) a reverse transcriptase reaction solution, c) reverse transcriptase, d) an RNA enzyme inhibitor, e) a primer and a TaqMan probe, f) a standard positive DNA template and g) a PCR fluorescence quantitative reaction solution. The kit is characterized in that: primer sequence is a sense primer: 5'-TGCGCCTATCCTTGAGTTGA-3', and an antisense primer: 5'-TTGCCAGGAAATACGGGTCT-3', and the size of an amplicon is 138 bp; the sequence of a fluorescence probe is: 5'-FAM-TCAAAATGGTGGACTTCAGTTTCGATTT-TAMRA-3', the 5' end of the probe is marked with a fluorescence emission group FAM, the 3' end is marked with a fluorescence quenching group TAMRA, the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with reovirus S1 protein zone 361 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The kit can efficiently and conveniently monitor the reovirus pollution in serum products in real time, can be applicable to epidemiology investigation of reovirus infection, and can provide technical support for relevant fundamental researches, thus having quite broad application prospect.

The invention discloses a rabies virus detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) an RNA extraction solution, b) a reverse transcriptase reaction solution, c) reverse transcriptase, d) an RNA enzyme inhibitor, e) a primer and a TaqMan probe, f) a standard positive DNA template, and g) a PCR fluorescence quantitative reaction solution. The kit is characterized in that: primer sequence is a sense primer: 5'-TAGGATGCTATATGGGTCAAGTCAGA-3', and an antisense primer: 5'-TTCAAATGTCCCTTTCCCGAAGAA-3', and the size of an amplicon is 125 bp; the sequence of a fluorescence probe is: 5'-FAM-CAACGGTTATTGCTGCATGTGCTCCTGA-TAMRA-3', the 5' end of the probe is marked with a fluorescence emission group FAM, the 3' end is marked with a fluorescence quenching group TAMRA, the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with rabies virus N protein zone 391 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The kit can efficiently and conveniently monitor the rabies virus pollution in serum products in real time, and can provide technical support for relevant fundamental researches, thus having quite broad application prospect.

The invention discloses a serum-free medium and a preparation method thereof, and belongs to the technical field of biochemistry. The preparation method comprises the following steps: 1, preparing an amino acid stock solution, a glutamine stock solution, a tryptophan and tyrosine disodium salt stock solution, a vitamin stock solution, an inorganic salt stock solution, a trace element stock solution and an insulin stock solution; and 2, adding a proper amount of potassium chloride, sodium chloride, and the amino acid stock solution, the tryptophan and tyrosine disodium salt stock solution, the vitamin stock solution, the inorganic salt stock solution, the trace element stock solution and the insulin stock solution which are prepared in step 1 into ultrafine water, adding sodium bicarbonate, carrying out mixing and dissolving to obtain a mixed solution, and adding the glutamine stock solution which is unfrozen by 37 DEG C water bath into the mixed solution before use in order to prepare the serum-free medium. The serum-free medium makes cell proliferation normal and the activity stable, is non-obviously different from a medium containing 10% of a finished calf serum product, has an obviously lower protein content than routine media, simplifies the extraction technology, and reduces the production cost.

Analysis of a goat serum product with many therapeutic effects is described. The product is identified as containing proopiomelanocortin (POMC) and Corticotropin releasing factor (CRF) peptides, as well as breakdown products of these peptides. We describe methods of treatment of diseases including cancers, multiple sclerosis, and neural disorders using these peptides and their products, as well as medicaments including such peptides and methods of producing the peptides.

The present invention relates to a plasma product or a serum product with an extremely low risk of viral contamination and a method for producing the same. Before treating plasma or serum to be used as a raw material for producing a plasma product or a serum product using a virus removal membrane, leucocytes contaminating the blood are removed. Thus, a plasma product or a serum product with an extremely low risk of viral contamination can be efficiently produced while preventing clogging. Since clogging scarcely arises, it is possible to carry out efficient filtration without applying an elevated pressure as the filtration proceeds.

The invention discloses a preparation method of a high-capacity serum antibody. The preparation method comprises the following steps of firstly, collecting immunized fresh blood, collecting blood plasma, and cryopreserving for later use; then, filtering the collected blood plasma, and collecting filtrate to obtain a primarily-filtered serum product; and adding thrombin into the prepared primarily-filtered serum product, uniformly stirring, warmly applying at the temperature of 20-37 DEG C for 0.5-4 hours, filtering by using filter paper with the aperture of 0.22-0.88mu m, and collecting filtrate to obtain the serum antibody. The serum antibody obtained by using the method disclosed by the invention is high in content and good in quality and stability; and the preparation method is suitable for preparing the high-capacity serum antibody and relatively good in market prospect.

The invention discloses a method for identifying agkistrodon halys venom by applying mass spectrometry. The method comprises the following steps of: (1) dissolving agkistrodon halys venom to prepare aprotein solution; (2) carrying out SDS-PAGE analysis; and (3) carrying out LC-MS/MS mass spectrometry to carry out agkistrodon halys venom component identification. In addition, the invention furtherdiscloses application of the identification method in the preparation of products for identifying the agkistrodon halys venom and application in the preparation of agkistrodon halys venom resistant serum for treating agkistrodon halys venom poisoning. According to the invention, the agkistrodon halys venom can be identified in a laboratory; agkistrodon halys venom is more accurately subjected toquality control, so that specific drug anti-agkistrodon halys poison serum for treating agkistrodon halys venom poisoning is produced; and the method is very important for ensuring the safety, effectiveness and quality controllability of anti-agkistrodon halys venom serum products and is of great significance for reference of the diagnosis and legal medical expert identification of the types of venom caused by snake venom poisoning. The method has the advantages of high detection sensitivity, high correct detection rate, no cross reaction and simple operation process.

The ratio of the metals zincand cadmium is of great significance for error-free proliferation and differentiation of cells. An understanding of the role of cadmium in the etiology of cancer offers possibilities to gain a better understanding of cancer as well as possibilities to prevent, treat and/or alleviate cancer. The concentration of Zn(II) in fetal serum, human serum from umbilical cord blood or healthy donors,and in avian serum including intact eggs and egg products has significance for the reliability and repeatability of cell and tissue culture method using said serum, or serum containing products,or eggs. Herein is disclosed a method step in the manufacture of serum products, in particular serum products intended for use in in vitroculture of mammalian cells or tissues, wherein the concentration of Zn(II) is determined, and preferably adjusted toa desired interval. Different products consisting of or containing serum or serum fractions are also disclosed.

The invention belongs to the field of antibodies, and particularly relates to an anti-human abnormal prothrombin antibody and an application thereof to preparation of a hepatocellular carcinoma detection kit. The anti-human abnormal prothrombin antibody can specifically bind human abnormal prothrombin, can further avoid interference of prothrombin from serum or plasma from the raw material level, can further avoid interference of abnormal prothrombin from other species in animal serum products, avoids related risks from the source, reduces reagent development difficulty, and has a good application prospect.

The present invention provides a composition and method for preparing a customized personal care product. The composition comprising a base composition and at least one additive compositions. The base composition is at least one or combination of organic or inorganic ingredients and organic origin ingredients, and the additive compositions include at least one hair care based additive compositions. at least one customized additive of predetermined composition from an ampoule is added to the base composition. The base composition and at least one customized additive compositions are mixed to form a customized personal care composition. The personal care product could be a health care product, beauty product, face serum product, body lotion product, hair care product, or any other product.

The invention discloses an anti-sea snake venom serum nano-membrane filtering method which comprises the following steps: (1) preparing an anti-sea snake venom serum chromatography flow-through liquid, namely obtaining plasma; digesting with pepsase; precipitating ammonium sulfate for the first time; precipitating ammonium sulfate for the second time; performing plate-frame pressure filtration; alum adsorption; performing plate-frame pressure filtration; performing supernate ultrafiltration, concentration and desalination; performing ion exchange column chromatography; collecting chromatography flow-through liquid; (2) controlling the environment temperature of nano-membrane filtration to be 20-28 DEG C, and preparing the chromatography flow-through liquid into a solution with a certain concentration and a certain prescription; and (3) carrying out nano-membrane filtration according to the conditions in the step (2). The chromatography flow-through liquid in the anti-sea snake venom serum process is subjected to nano-membrane filtration virus removal, optimal conditions are obtained through multiple tests, the flux of nano-membrane filtration is increased, the production cost is greatly reduced, the virus removal efficiency of anti-sea snake venom serum products is improved, and the application of nano-membrane filtration in anti-sea snake venom serum production is accelerated.

A preparation method of antitoxic serum for octanoic acid purification treatment comprises steps of pepsin digestion, first precipitation and heating degeneration treatment, second precipitation treatment, alum precipitation treatment, ultrafiltration concentration treatment, and stock solution preparation. An octanoic acid purification step is added in the preparation method, so that an antitoxic serum product has high quality indexes and safe usage. Compared with an existing preparation process, in main quality indexes of the antitoxic serum product, content of F(ab)2 is increased to above 80% from 50%-60%, purity specific activity (unit/g protein) is improved to above 78000 from 35000-45000, and allergic reaction incidence and serum sickness incidence of the antitoxic serum product are reduced by 30%-40% during clinical application.

The invention discloses a quality control method of sea snake venom and application thereof. The quality control method comprises the following steps of: (1) selecting freeze-dried powder of sea snake venom (containing Lapemis curtus venom and Hydrophis cyanocinctus venom) from the origin of China, dissolving the freeze-dried powder and preparing into a protein solution; and (2) performing high performance liquid chromatography analysis with different wavelengths. In addition, the invention further discloses application of the quality control method of the sea snake venom in preparation of anti-sea snake venom serum for treating sea snake venom poisoning. According to the quality control method, the quality of the sea snake venom can be controlled, the specific medicine anti-sea snake venom serum for treating sea snake venom poisoning is further produced, and the method has very important significance for ensuring that the anti-sea snake venom serum product is safe, effective and controllable in quality. The quality control method is good in detection repeatability, high in reliability, simple in operation process, quicker, more efficient and low in cost.