Quality control method of sea snake venom and application thereof

A quality control method and snake venom technology, applied in the direction of anti-animal/human immunoglobulin, measuring devices, instruments, etc., can solve the problems of long test time, expensive instruments and equipment, etc., achieve rapid comparison and identification, low cost, and reliable highly operable effect

Pending Publication Date: 2021-12-17
中国人民解放军海军特色医学中心 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Invention patent application CN 110426522 A discloses a method for identification of Viper venom and its application. SDS-PAGE electrophoresis combined with mass spectrometry is used to identify the venom of Viper venom. The identification method can clearly identify the type of protein , but the equipment is expensive and the test time is long

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  • Quality control method of sea snake venom and application thereof
  • Quality control method of sea snake venom and application thereof
  • Quality control method of sea snake venom and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 A kind of quality control method of sea snake venom

[0040] Proceed as follows:

[0041] (1) Prepare sea snake venom solution A (referred to as solution A), that is, 3.5g sodium chloride, 4.2g boric acid, 0.49g sodium tetraborate, add water for injection to 1L, and adjust the pH value to 7.2;

[0042] (2) Dissolve the freeze-dried powder of sea snake venom (including sea snake venom and sea snake venom) with the above solution A, and prepare a protein solution with a protein concentration of 13 mg / ml;

[0043] (3) Select the analytical column G3000SWxl, adjust the flow rate to 0.6 ml / min, and equilibrate for 40-60 min until the baseline is stable and no longer fluctuates;

[0044] (4) After the dissolved protein solution is filtered through a 0.22 μm filter membrane, put it into the sample holder, and set the sample loading volume to 20 μl.

[0045] (5) The wavelength for reading the absorbance is set to two wavelengths of 215 nm and 280 nm.

[0046] (6) ...

Embodiment 2

[0048] Example 2 Application of a quality control method for sea snake venom in the preparation of anti-sea snake venom serum for the treatment of sea snake venom poisoning

[0049] Proceed as follows:

[0050] (1) The lyophilized powder of sea snake venom (including sea snake venom and sea snake venom) was dissolved in solution A prepared in Example 1, and analyzed by high performance liquid chromatography;

[0051] (2) The HPLC analysis results ( Figure 2A , Figure 2B , Figure 2C , Figure 2D ) with the existing map ( Figure 1A , Figure 1B , Figure 1C , Figure 1D ) comparison to confirm the number of protein peaks at 215 nm and 280 nm. The peaks of the flat-chinned sea snake venom are 13 and 7 at 215 nm and 280 nm, respectively, and the peaks of Qinghuan sea snake venom are 12 at 215 nm and 280 nm. and 9. ;

[0052] (3) After the sea snake venom that has been analyzed for quality control is detoxified by formaldehyde, the horses are immunized;

[0053] (4) H...

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Abstract

The invention discloses a quality control method of sea snake venom and application thereof. The quality control method comprises the following steps of: (1) selecting freeze-dried powder of sea snake venom (containing Lapemis curtus venom and Hydrophis cyanocinctus venom) from the origin of China, dissolving the freeze-dried powder and preparing into a protein solution; and (2) performing high performance liquid chromatography analysis with different wavelengths. In addition, the invention further discloses application of the quality control method of the sea snake venom in preparation of anti-sea snake venom serum for treating sea snake venom poisoning. According to the quality control method, the quality of the sea snake venom can be controlled, the specific medicine anti-sea snake venom serum for treating sea snake venom poisoning is further produced, and the method has very important significance for ensuring that the anti-sea snake venom serum product is safe, effective and controllable in quality. The quality control method is good in detection repeatability, high in reliability, simple in operation process, quicker, more efficient and low in cost.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for quality control of sea snake venom, in particular to a method for monitoring the quality of snake venom using snake venom protein analysis technology; in addition, the invention also relates to the application of the quality control method, which is beneficial to the immunity of horses The quality stability and batch consistency of sea snake venom ensure the stable quality, safety and effectiveness of anti-sea snake venom products. Background technique [0002] Venomous snakebites are common in subtropical and tropical regions. In the rural areas of Asia, Africa, Latin America and Oceania, venomous snakebite is a particularly prominent public health problem, and the incidence of snakebite in my country shows a trend of increasing year by year. [0003] The venom is mostly light yellow or milky white translucent viscous liquid with more than 100 components. Each snak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74C07K16/18C07K16/06
CPCG01N30/02G01N30/74C07K16/18C07K16/06
Inventor 张黎明杨智柳国艳范铁炯王博周东王蓓蕾陈则
Owner 中国人民解放军海军特色医学中心
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