Methods and compositions for use in the prevention, treatment and/or alleviation of cancer

A species, mammalian technology, applied in biochemical equipment and methods, drug combinations, medical raw materials derived from mammals, etc., can solve problems such as fear of cancer diagnosis and indeterminate results

Inactive Publication Date: 2018-08-31
马蒂 J 西伦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While some forms of cancer can be cured, the results are far from

Method used

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  • Methods and compositions for use in the prevention, treatment and/or alleviation of cancer
  • Methods and compositions for use in the prevention, treatment and/or alleviation of cancer
  • Methods and compositions for use in the prevention, treatment and/or alleviation of cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1. Analyze the Zn(II) concentration in the serum of different sources

[0117] The inventors commissioned the analysis of different batches of sera and also sera from different animals. These analyzes of different batches of FBS showed significant differences in Zn(II) concentration between batches. Analysis of sera from different animals showed significant differences between animals.

[0118] Analysis was performed at an accredited laboratory using atomic absorption spectrometry (AAS) with standard samples obtained from Perkin-Elmer (Norwalk, CT, USA). The results are shown in Table 2.

[0119] Table 2. Zn(II) Concentrations in Serum Samples

[0120]

[0121]

[0122] Overall, the analysis revealed that there are indeed considerable differences in Zn(II) concentrations in serum of different origins, both geographical and from different animal species. In cell culture, serum and especially fetal calf serum is a standard component, usually added...

Embodiment 2

[0124] Embodiment 2. Analysis of the Cd concentration in the fetal serum sample

[0125] The inventors also commissioned the analysis of cadmium in a number of serum samples. To the best of its knowledge, cadmium has no known beneficial function in the human body. However, there is epidemiological evidence showing a significant association between cadmium and cancer in humans.

[0126] Concentrations of cadmium were determined at an accredited laboratory (VITA Laboratorio, VITA-Terveyspalvelut, Helsinki, Finland) using standard procedures with an atomic absorption spectroscopy (AAS) instrument from Perkin-Elmer (Norwalk, CT, USA). The results showed that the fetal serum was free of cadmium or contained only cadmium at a level below the detection limit of the analytical method, here 4.5 μmol / l. The results are shown in Table 3.

[0127] Table 3. Comparison of concentrations of Zn(II) and Cd in serum samples

[0128]

[0129] It is generally considered that non-smoker...

Embodiment 3

[0132] Example 3. First test, isolation of particles from serum of different origin

[0133] The present inventors also commissioned a study of ultracentrifugation of cell-free serum samples. The study was carried out in November 2015 at VTT Technical Research Center Finland Ltd. in Espoo, Finland. An ultracentrifuge (Beckman Optima(TM) LE-BOK) was used according to the manufacturer's instructions. The aim of the study was to test whether particles smaller than 200 nm (0.2 μm) in diameter could be separated from cell-free serum samples using ultracentrifugation.

[0134]A total of 8 samples were supplied in duplicate and stored at -20°C according to the inventor's instructions. The inventors also supplied stabilizers (DTT, DL-dithiothreitol) and stored at +4°C. The samples were ultracentrifuged at 150 000 × g for 20 h at +4 °C. During ultracentrifugation, one test tube ruptured, but testing showed precipitate formation in all test tubes except this one. It was noted tha...

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Abstract

The ratio of the metals zincand cadmium is of great significance for error-free proliferation and differentiation of cells. An understanding of the role of cadmium in the etiology of cancer offers possibilities to gain a better understanding of cancer as well as possibilities to prevent, treat and/or alleviate cancer. The concentration of Zn(II) in fetal serum, human serum from umbilical cord blood or healthy donors,and in avian serum including intact eggs and egg products has significance for the reliability and repeatability of cell and tissue culture method using said serum, or serum containing products,or eggs. Herein is disclosed a method step in the manufacture of serum products, in particular serum products intended for use in in vitroculture of mammalian cells or tissues, wherein the concentration of Zn(II) is determined, and preferably adjusted toa desired interval. Different products consisting of or containing serum or serum fractions are also disclosed.

Description

technical field [0001] The present disclosure investigates the etiology of cancer and presents new principles for preventing, treating or ameliorating cancer in mammalian subjects. [0002] The present disclosure also relates generally to improved production and use of blood products, serum products, and cell growth media, and in particular to such products that consist of or include fetal serum of various origins, but are not limited to here. Avian serum and egg products are also relevant growth media that can be improved based on the findings and methods disclosed herein. [0003] Also included herein are blood products intended for administration to human patients, such as whole blood products, serum and plasma products, and blood components. Background technique [0004] Cancer is a collective term for diseases caused by uncontrolled cell division in the body. Usually, the term refers specifically to malignant growths or tumors that result from this uncontrolled cell ...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K33/30C12N5/00A61P39/00
CPCA61K45/06C12N5/0018C12N2500/10C12N2500/22A61K33/30A61K35/16A61P35/00A61P39/00A61P43/00A61K2300/00A61K35/14
Inventor 马蒂·J·西伦
Owner 马蒂 J 西伦
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