The invention is suitable for the technical field of an illumination spectrum, and provides a comprehensive evaluation optimization method for a light source vision and non-vision effect performance. The comprehensive evaluation optimization method comprises the following steps of obtaining a non-vision biological effect matching function c<->(lambda), a scotopic vision matching function s<->(lambda) and a photopic vision matching function p<->(lambda) according to a spectrum-sensitive curve of a non-vision biological effect C(lambda), a scotopic vision V'(lambda) and a photonic vision V(lambda); according to the c<->(lambda), the s<->(lambda) and the p<->(lambda) and the arrangement relationship of light source spectrums, obtaining radiation efficiency functions of the C(lambda), the V'(lambda) and the V(lambda), and performing unification for obtaining three stimulus values C, S and P which correspond with the C(lambda), the V'(lambda) and the V(lambda); calculating c, s and p, and establishing a CSP spectrum locus diagram according to the c, the s and the p; drawing a coordinate which corresponds with an equal-energy white and black-body radiation light source on the CSP spectrum; and drawing an S / P value spectrum and a C / P value spectrum on the CSP spectrum. According to the comprehensive evaluation optimization method, related visions are integrated and unified, thereby facilitating comprehensive performance improvement of different visions and realizing accurate calculation; and furthermore light source spectrum optimization is facilitated, thereby satisfying a requirement for required visual performance.