54 results about "Salt-tolerant crops" patented technology

The invention discloses a method for identifying quality of cotton salt-tolerant seeds. Salt-tolerant seeds are screened by adopting an indoor experiment and an outdoor experiment, wherein the indoor experiment comprises an indoor germination experiment I and / or an indoor germination experiment II; and the outdoor experiment comprises outdoor salt-tolerance identification I and / or outdoor salt-tolerance identification II. In the invention, the salt-tolerance character of cotton is integrally identified and evaluated by adopting double-layered filter papers, sand, salt ponds and heavy alkali-salinity soils by the seaside. The salt-tolerance in the germination stage, the seedling stage and the plant-forming stage of cotton can be identified and evaluated. The salt-tolerance of cotton is detected roundly and stably with high efficiency and high throughout. The integral identification and evaluation of multi-stage, multi-place and multi-level can be performed for the salt-tolerance of crops which are controlled by complicated quantitative inheritance genes and greatly influenced by external environment.

The invention discloses a method for comprehensive treatment of crop planting in saline-alkali lands, which is used for the treatment and the planting in the saline-alkali lands and comprises the steps: (a) irrigation and salt elimination comprise underground salt elimination and ground surface salt elimination which are completed by three steps, wherein the underground salt elimination is firstly carried out for at least three days, the ground surface salt elimination is then carried out, and the underground salt elimination is repeated for 1-3 days at the third step; (b) the planting and salt reduction are realized by that a large area of suaeda salsa is planted, the irrigation and the salt elimination are conducted continuously before sprouting of the suaeda salsa and within two weeks after the sprouting, and the slightly wet irrigation is carried out at an interval when branching of the suaeda salsa is begun till the end of a growth peak period of the suaeda salsa; and (c) the planting of salt-tolerant crops is completed by that the slightly wet irrigation is continued for at least three days through the underground salt elimination, the large-area ground surface salt elimination is conducted for one time quickly, wheat is then seeded, the slightly wet irrigation is continued within one week after the seeding and after the sprouting, large flow irrigation is carried out for one time before the winter, and the slightly wet irrigation is carried out at an interval till the wheat gets ripe.

The invention discloses a plant electric signal based crop salt tolerance evaluation method. The method specifically comprises the steps of by acquiring surface potential signals of a germinated seed of a crop before and after salt stress, calculating characteristic values of the potential signals before and after the salt stress; and according to the changes of the characteristic values, defining a salt tolerance evaluation coefficient of the crop for evaluating the salt tolerance of the crop. According to the method, during crop salt tolerance evaluation, only a small amount of crop seedlings need to be subjected to the salt stress without a field experiment, the surface potential fluctuation signals of the seedlings are tracked and measured, and the characteristic values of the electric signals are calculated to evaluate the salt tolerance of the crop nondestructively and quickly. According to the method, the seed consumption is low and seeds do not need to be destroyed; and the method has the characteristic of nondestructive measurement. In addition, the method is used for tracking and measuring the electric signals of the seeds in the same batch, and the evaluation method is used for performing comparison according to the change degrees of the electric signal parameters of the crop before and after the salt stress, so that the individual difference of the seeds is avoided, culture and measurement methods are simple, and the evaluation effect is reliable.

The invention discloses a growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands. The growth-promoting bacteria combination comprises a bacillus amyloliquefaciens strain ZCM18 and a bacillus tequilensis strain. Preservation numbers of the two strains are CGMCC No.1295 and CGMCC No.12960 respectively. The two strains are capable of tolerate the salinity of 5%, have high capabilities of phosphate solving, nitrogen fixing and producing IAA (indoleacetic acid), ACCD (acetyl-CoA carboxylase subunit D), siderophore and cytokinin, and can antagonize root pathogenic bacteria including fusarium, rhizoctonia, pythium and the like. The two stains after being combined are suitable for the moderate-severe saline and alkaline lands (with salt content ranging from 0.4% to 1.0%), and in the production field of biological fertilizer for salt tolerating and growth benefiting for the crops, the growth-promoting bacteria combination is promising in prospects of crop's salt tolerance enhancement, crop root growth benefiting, seedling existing rate increasing, fertilizer using amount decreasing and crop yield increasing.

The invention discloses a wheat salt-tolerant gene pyruvic acid transporter gene, and particularly relates to an allene oxide reductase gene TaBASS2 and a plant expression vector containing the gene TaBASS2. The invention further discloses an application of the gene TaBASS2 in cultivation of salt-tolerant plants, especially wheat. An experiment proves that the salt tolerance of a transgenic plant prepared by transgene transformation disclosed by the invention is obviously improved; and a foundation is laid for wide application of the gene in cultivation of a new variety of salt-tolerant crops.

The invention discloses application of a phospholipase gene PLDzeta1 in improving salt resistance of a plant. The gene PLDzeta1 is transferred to a plant through a molecular genetic transformation method, and the transformation plant with the overexpression gene PLDzeta1 is obtained. The overexpression gene PLDzeta1 remarkably improves the salt resistance of the plant. The membrane permeability and the malondialdehyde content of a leaf of the plant with overexpression gene PLDzeta1 are remarkably lower than those of a control wide group under the threat of salt, and the survival rate and the plant height are remarkably higher than those of the control wide group. The loss expression of the gene PLDzeta1 leads to remarkable reduction of the salt resistance of the plant and remarkable reduction of biological yield compared with the wild group, and the result further improves that the gene PLDzeta1 has a remarkable positive regulation effect on salt resistance. The application of the phospholipase gene PLDzeta1 in improving salt resistance of the plant provides a novel way for the seed breeding of salt-resistance molecules of the plant, and creates a novel seed material.

The invention discloses a salt-tolerant gene for wheat, a plant expression vector and particular application of the gene TaAOC1. The salt-tolerant gene is allene oxide reduction enzyme gene TaAOC1 and is applied to cultivating salt-tolerant plants, in particular to cultivating wheat, and the plant expression vector comprises the gene TaAOC1. The salt-tolerant gene TaAOC1, the plant expression vector and the application have the advantages that as proved by experiments, the salt tolerance of transgenic plants which are subjected to transgenosis by the aid of the salt-tolerant gene is obviously improved, and a foundation is laid for widely applying the gene to cultivating new species of salt-tolerant crop.

The invention provides a screening method of a growth-promoting bacterial strain for improving salt tolerance of crops. The screening method belongs to the technical field of microbe separation and application, and comprises the two steps of screening dominant strains and combining the dominant strains. The screening of the dominant strains is characterized by screening a series of salt-tolerance and growth-promoting functions so as to finally obtain the bacterial strains with multiple functions or superior advantages; the combining of the dominant strains is characterized by following the principle that the bacterial strains are in mutualism and functional complementation so as to provide the colonization and the function expression ability of bacterial strain combination in crop rhizospheres and realize an effect of 'one plus one is more than two'. The composite microbial system obtained through screening by the method provided by the invention has an outstanding field application effect, solves the problems that in similar research, the bacterial strain is single or adopts a simple combination method, so that the field application effect is instable and poor in effect, and is beneficial for greatly promoting the production of saline soil crops.

The invention discloses a rhizosphere growth-promoting bacterium for enhancing the salt tolerance of crops and microbial fertilizer and application of the bacterium. The preservation number of provided bacillus paralicheniformis T1-8 is CGMCC No. 17335. The bacillus paralicheniformis T1-8 is inoculated to the rhizospheres of potted corn and wheat, and the growth indexes of the corn and wheat can be increased under salt stress. The microbial organic fertilizer is prepared by means of secondary fermentation after a bacterial liquid fermented by the bacterium is uniformly mixed with common cow manure, common pig manure and straw for composting, and can increase the yield of corn on saline-alkali land and improve the physical and chemical properties of soil to a certain extent. The bacterium has important significance for the development of functional microbial organic fertilizer capable of enhancing the salt tolerance stress of crops and increasing the yield of the crops on saline-alkaliland.

The invention relates to a salt tolerant gene HgS2 derived from halophyte halogeton glomeratus, and application thereof. The invention aims at providing a novel salt tolerant gene HgS2, encoding protein of the salt tolerant gene HgS2, and application of the salt tolerant gene HgS2 to improve the plant salt tolerance and culture a salt tolerant new variety (strain). The salt tolerant gene HgS2 contains a nucleotide sequence of SEQ ID NO.1cDNA, and the molecular weight is 678bp; the cDNA coding sequence is the nucleotide sequence from the 30th site to the 290th site in the SEQ ID NO.1, and the molecular weight is 261bp; the amino acid sequence is shown as SEQ ID NO.3 and consists of 86 amino acids. The salt tolerant gene HgS2 can obviously improve the salt tolerance of arabidopsis thaliana plants. The salt tolerant gene provided by the invention is favorable for the culture of salt tolerant crops and plant new varieties (strains).

The invention discloses a saline-alkali land clayey soil field salinity regulation and control method. The method comprises the steps that average water content of soil 0.5 deep is determined through selecting a saline-alkali land parcel and constructing lateral seepage prevention facilities and water drainage and salt elimination facilities, salt water mineralization degree blending is conducted through utilizing a linear relation between the salt water mineralization degree and soil total salt content, and the blended salt water is used for conducting irrigation, so that the soil salt content reaches a preset concentration, and the purposes of gradient salinity identification and selection of salt tolerance of crops are achieved. The saline-alkali land clayey soil field salinity regulation and control method is simple, convenient and easy to operate, and the method has a larger usage value in saline-alkali land selection of salt-tolerant crops and salt-tolerant crop breeding application.

The invention discloses a sedum lineare salt-tolerant gene SlWRKY and an application thereof. The nucleotide sequence of the sedum lineare salt-tolerant gene SlWRKY is shown in SEQ ID NO. 1; experiments prove that the tolerance of arabidopsis thaliana and poplar transfected by the SlWRKY gene to salt is enhanced, the sedum lineare provided by the invention is indicated to play an important role inimproving the salt tolerance of crops by enhancing the salt-tolerant gene SlWRKY.

The present invention is cord grass sodium hydrogen pump protein gene SaNHX and its application. The sodium hydrogen pump protein gene SaNHX has the DNA sequence as shown in sequence 1 of the sequence list or the DNA sequence with over 90 % homology with the sequence 1 limited DNA sequence and coding protein with the same function. The gene SaNHX coded cord grass sodium hydrogen pump protein is protein with the amino acid residue sequence as shown in sequence 2 of the sequence list or derivative protein of the sequence 2 through the substitution, deletion or addition of one or several amino acid residues and with the same activity as the protein with the amino acid residue sequence in sequence 2. The gene of the present invention is significant in cultivating new variety of salt tolerant crop, especially salt tolerant rice.

The invention discloses a salt-induced functional element of a high-efficiency soybean promoter. The element is named as the salt-induced functional element GmBM1 of the high-efficiency soybean promoter, and a nucleotide sequence of the element is as shown as SEQ ID No. 1 (sequence identifier number 1). The invention further discloses a plant bifluorescence report carrier pGmBM1-pGreen II-0800 LUC comprising the salt-induced functional element and an application of the salt-induced functional element in improving salt stress tolerance of a plant. Experiments prove that the salt-induced functional element can quickly and efficiently respond to high salt induction, which indicates that the salt-induced functional element can be widely used for relevant functional studies of cultivating salt-resistant plant species and genetically modified salt-tolerant crops.

The invention provides a salt tolerance screening and identification method for corn in the seedling stage. It is found and verified that salt tolerance and zinc sensitivity of a corn selfing line are consistent. Whether a corn variety is tolerant to salt is judged primarily according to whether corn seedlings subjected to zinc deficiency stress treatment have zinc deficiency symptoms or not, further, zinc stress treatment is conducted on added zinc subjected to zinc deficiency stress treatment to further judge whether the corresponding corn variety is tolerant to salt, the two steps are combined, and screening and identification precision is improved. Besides, the method is practical and effective, operation is easy to implement, chemicals are not needed for detection, and accordingly cost is low. The method is free of toxin and harm to human bodies and free of environment pollution, and lays a certain theoretical foundation for large-scale screening of corn salt-tolerant selfing lines and breeding of salt-tolerant crops.

The invention discloses a wheat salt-tolerant gene TaCYP81 and an application thereof. A nucleotide sequence of cDNA (Complementary Deoxyribonucleic Acid) of the wheat salt-tolerant gene TaCYP81 is as shown in SEQ ID No.1. The invention also discloses plant expression vectors Prok2-TaCYP81 and pGA3626-TaCYP81 containing the gene. Meanwhile, the invention provides an application of the gene TaCYP81 to cultivation of salt-tolerant plants. Experiments prove that the salt tolerance of the obtained transgenic plant is remarkably improved, which indicates that the wheat salt-tolerant gene TaCYP81 can be widely applied to cultivation of salt-tolerant crops and particularly salt-tolerant wheat varieties.

The invention discloses a soil improvement method for restoring saline-alkali soil based on a biological method, and relates to the technical field of soil improvement. In order to solve the problem that ardealite easily causes soil heavy metal pollution, the method specifically comprises the following steps of preparing the saline-alkali soil; dividing the saline-alkali soil into regions, and detecting the PH value and the EC value of the soil in each region; digging pits in the surface of the saline-alkali soil, and leaching the saline-alkali soil in rainy seasons; uniformly spreading diluted microbial fertilizer on the surface of the saline-alkali soil and in the pits; performing deep ploughing and deep scarification of saline-alkali soil; performing land leveling; reasonably distributing and planting salt-tolerant crops; and performing intertillage of the saline-alkali soil before the salt-tolerant crops are reasonably distributed and planted. The depth of the deep ploughing and deep scarification saline-alkali soil is 50-60 cm. The saline-alkali soil subjected to biological improvement has the effects of being durable and stable in desalination and beneficial to water and soilconservation and ecological balance, microbial activities are promoted, the physical and chemical properties of the soil are further improved, and heavy metal pollution of the soil cannot be caused.

The invention relates to the technical field of saline-alkali soil treatment, in particular to a method for comprehensively treating saline-alkali soil. The method comprises the following steps of S1,engineering treatment, namely establishing a modern drainage system; S2, agricultural measures, namely including tillage, soil dressing, fertilization, sowing, crop rotation, intercropping, interplanting and rice planting; S3, biological measures, namely including salt-tolerant crop planting, green manure forage grass planting and afforestation; and S4, chemical measures, namely in the treatmentprocess, insisting on the basis of drainage, comprehensively treating the relationship of drainage, irrigation, storage and supplementation, correctly selecting the critical depth of an underground water level, performing irrigating in the drought period, performing draining in the waterlogging period, controlling the underground water level in the period of salt return, and supplementing a watersource after the underground water level is lowered due to the fact that the water source is filled, so that regional water-salt movement can be in a good state so as to achieve the aim of improving the soil. Engineering measures and various measures such as agriculture and biology are closely combined, a good farmland ecosystem of water, fertilizer, gas and heat necessary for crop growth is established according to local conditions, and the purposes of improving soil fertility and increasing agricultural yield are achieved.

The invention discloses a plant salt-tolerance-related protein AtSDIP1, and a coding gene and an application thereof. The protein provided by the invention is the following (a), or (b), or (c): (a) a protein composed of an amino acid sequence represented by the sequence 1 in the sequence table; (b) a protein derived from the sequence 1 and is related to plant salt tolerance, wherein the amino acid sequence of the sequence is subjected to substitution and / or deletion and / or addition of one or more amino acid residues; (c) a protein derived from the sequence 1 and is relates to plant salt tolerance, wherein the protein has at least 50%, at least 60%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 90%, at least 95%, at least 98%, or at least 99% homology with the sequence 1. The invention provides a basis for the researches of plant response and tolerability molecular mechanisms against salt stress, and has important significance to crop improvement and salt-tolerant crop cultivation.

The invention discloses a method for identifying the salt tolerance of cotton in a seedling period. The method comprises the specific steps: planting box preparation, field digging, soil sieving, boxing, pit filling, watering before sowing, film mulching, sowing and film mulching, film uncovering, salt application, investigation statistic and expert conclusion. Judged by a relative survival ratio,the salt tolerance of cotton varieties is divided into four grades, finally, the salt tolerance of each variety under salt application levels of different treatments is listed, and furthermore, the salt tolerance range and limit of each variety are obtained. Preferably, the salt application levels of all the treatments are 0, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75% and 0.8%; and the length, width and height of a planting box are respectively 90cm, 30cm and 30cm. The method is low in facility / equipment requirement, low in cost, abundant in data and reliable in result. The method isnot only suitable for cottons, and a design thought of the method can be referred, popularized and applied to identification of salt tolerance of crops such as wheat and corns.

The invention discloses a Glycine max(L.)Merr Williamus 82 medium-small G protein gene, the gene is named as Glycine max(L.)Merr Williamus 82NAC membrane-bound transcription factor gene GmRAB7, and anucleotide sequence of cDNA of the gene is shown as SEQ ID No.1. The invention also discloses an application of the gene in improving the salt tolerance of plants. The experiments prove that the salttolerance of the GmRAB7 transgenic plant is greatly improved compared with that of a non-transgenic plant, the gene is expected to play an important role in cultivating salt-tolerant plant varieties,and a new theoretical basis and a new practical basis are provided for improving the salt tolerance of crops.

The invention relates to the application of tomato TFT1 gene to improving the salt tolerance of crops, comprising the following steps of: obtaining the DNA sequence of TFT1, cloning the encoding area of TFT1 gene in a tomato body through the PCR method, connecting the encoding area to a modified vector pBI121 after sequencing identification (see figure 1), and transforming targeted crops through the floral dip method. The crops in which TFT1 is transplanted are all along endowed with higher APX activity no matter under a normal growth condition or salt stress condition, thereby indicating that the TFT1 has the function of improving the salt tolerance of crops.

The invention discloses a method for evaluating crop salt tolerance by utilizing external light to induce a seed to illuminate. The method specifically comprises the following steps of respectively measuring spontaneous luminescence intensities of an ungerminated seed and a salt-stressed germinated seed, respectively measuring luminescence intensity attenuation curves of the ungerminated seed and the salt-stressed germinated seed under the irradiation of the external light, and obtaining delay luminescence kinetic parameters of the ungerminated seed and the germinated seed; evaluating the salt tolerance of the seeds according to the variation of the kinetic parameters of the luminescence intensity attenuation curves. According to the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate, when a crop salt tolerance evaluation is performed, field trials are unnecessary, only a few germinated seeds are salt-stressed necessarily, the luminescence attenuation curve of the germinated seed, which is induced by the external light, is measured in a track manner, the salt tolerance of the seed is losslessly and quickly evaluated by means of extracting and analyzing the kinetic parameters of the luminescence attenuation curves, and the features of less seed usage and nondestructive measurement are obtained. In addition, the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate is used for tracking and measuring the same batch of seeds, and evaluating the seeds according to the variation degree of the seed spontaneous luminescence parameters, so that the evaluation result is more reliable.

The embodiment of the invention discloses a medium trace element soil conditioner and a preparation method thereof. The medium trace element soil conditioner comprises the following components in parts by mass: 20-70 parts of oyster shell, 10-30 parts of silicon-based non-metallic material, 10-30 parts of light calcined magnesia powder and 5-10 parts of bentonite. The soil conditioner disclosed bythe invention not only contains organic matter for improving soil, but also contains medium trace elements required in a plant growth process, so that the planting cost is effectively reduced; secondly, the soil conditioner activated through high-temperature calcination can effectively improve the pH value of acid soil and avoid loss of medium trace elements, so that the effects of conditioning the soil, complexing and precipitating heavy metals, facilitating growth and reproduction of microorganisms, loosening and ventilating the soil, balancing the mineral content of the soil, improving beneficial salt bases and enhancing the salt tolerance of crops are achieved; and in addition, the raw materials of the soil conditioner are cheap and easy to obtain, exploitation is not needed, the preparation method is simple, cost is low, and the soil conditioner has great application and popularization prospects.

The invention discloses a method for comprehensive treatment of crop planting in saline-alkali lands, which is used for the treatment and the planting in the saline-alkali lands and comprises the steps: (a) irrigation and salt elimination comprise underground salt elimination and ground surface salt elimination which are completed by three steps, wherein the underground salt elimination is firstly carried out for at least three days, the ground surface salt elimination is then carried out, and the underground salt elimination is repeated for 1-3 days at the third step; (b) the planting and salt reduction are realized by that a large area of suaeda salsa is planted, the irrigation and the salt elimination are conducted continuously before sprouting of the suaeda salsa and within two weeks after the sprouting, and the slightly wet irrigation is carried out at an interval when branching of the suaeda salsa is begun till the end of a growth peak period of the suaeda salsa; and (c) the planting of salt-tolerant crops is completed by that the slightly wet irrigation is continued for at least three days through the underground salt elimination, the large-area ground surface salt elimination is conducted for one time quickly, wheat is then seeded, the slightly wet irrigation is continued within one week after the seeding and after the sprouting, large flow irrigation is carried out for one time before the winter, and the slightly wet irrigation is carried out at an interval till the wheat gets ripe.

The invention discloses a nutrition additive for improving the salt tolerance of crops in seedling growing periods. The nutrition additive has the advantages that hyphae for the nutrition additive can adapt to salinity soil, P, Cu and Mg absorption of the crops can be improved when the nutrition additive is used in the seedling growing periods, Na and Cl absorption of the crops can be reduced, and accordingly the salt tolerance of the crops can be improved; weathered coal, cottonseed cake and the like are subjected to mixed fermentation, and accordingly a material basis can be provided for growth of the hyphae; the nutrition additive can be used as organic biomass, accordingly, the base exchange capacity of the soil can be improved, the fertilizer conservation capacity of the soil can be guaranteed at seedling stages, salinity of the soil can be improved, and effects of promoting growth of the crops can be realized.