Method for evaluating crop salt tolerance by utilizing external light to induce seed to illuminate

Abstract

The invention discloses a method for evaluating crop salt tolerance by utilizing external light to induce a seed to illuminate. The method specifically comprises the following steps of respectively measuring spontaneous luminescence intensities of an ungerminated seed and a salt-stressed germinated seed, respectively measuring luminescence intensity attenuation curves of the ungerminated seed and the salt-stressed germinated seed under the irradiation of the external light, and obtaining delay luminescence kinetic parameters of the ungerminated seed and the germinated seed; evaluating the salt tolerance of the seeds according to the variation of the kinetic parameters of the luminescence intensity attenuation curves. According to the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate, when a crop salt tolerance evaluation is performed, field trials are unnecessary, only a few germinated seeds are salt-stressed necessarily, the luminescence attenuation curve of the germinated seed, which is induced by the external light, is measured in a track manner, the salt tolerance of the seed is losslessly and quickly evaluated by means of extracting and analyzing the kinetic parameters of the luminescence attenuation curves, and the features of less seed usage and nondestructive measurement are obtained. In addition, the method for evaluating the crop salt tolerance by utilizing the external light to induce the seed to illuminate is used for tracking and measuring the same batch of seeds, and evaluating the seeds according to the variation degree of the seed spontaneous luminescence parameters, so that the evaluation result is more reliable.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology and relates to a method for evaluating the salt tolerance of crops by using external light to induce seed luminescence. Background technique [0002] With the rapid development of social economy, various natural and human factors make soil salinization more and more serious. 20% of the world's arable land has varying degrees of salinization, which is widely distributed in more than 100 countries and regions. The salinization of a large amount of soil poses a great threat to global food security and has become one of the most important ecological problems that seriously restrict agricultural production and human survival. In this situation, using various means to control and improve salinized soil, develop and cultivate salt-tolerant crops, improve the utilization rate of salinized soil, and maintain food production have become major issues for human beings today. [0003] In order t...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for evaluating the salt tolerance of crops by using external light to induce seed luminescence, which solves the problem that the existing crop salt tolerance evaluation method has a large amount of samples, a long cycle, cannot be diagnosed early, and is not suitable for destructive measurement. Problems with Rare Species Evaluation

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