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Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands

A technology of severe saline-alkali land and growth-promoting bacteria, which is applied in the direction of plant growth regulators, plant growth regulators, and microbial-based methods, and can solve problems such as unsatisfactory effects, limited application range, and instability

Active Publication Date: 2017-05-10
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research mostly focuses on a single strain, and the effect is often unsatisfactory or unstable, and the scope of application is also limited. Therefore, research on multi-bacteria combinations with diverse functions and a wide range of applications is the research direction.

Method used

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  • Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands
  • Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands
  • Growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of Bacillus amyloliquefaciens ZCM18

[0032] 1. Isolation of strains

[0033] Take 1 g of wheat rhizosphere soil from saline-alkali land in Haixing County and put it into 10 mL of sterile saline, and shake it with a vortex instrument for 1 min to prepare a rhizosphere soil bacterial suspension. The bacterial suspension was serially diluted, spread on LB plates containing 5% NaCl, and cultured in a 30°C incubator for 48 hours to isolate bacterial strains.

[0034] 2. Morphological characteristics of the strain

[0035] Form round, flat, moist and viscous colonies on LB plate, grow turbidly in LB liquid medium, and form bacterial film in the later stage of culture. After Gram staining, it can be seen under the microscope that the ends are blunt and round, of different lengths, Gram-positive bacilli, and there are subterminal spores that are larger than the bacteria.

[0036] 3. Physiological and biochemical characteristics of the ...

Embodiment 2

[0045] Example 2 Isolation and Identification of Bacillus Tequila ZCM5

[0046] 1. Isolation of strains

[0047] Take 1 g of wheat rhizosphere soil from saline-alkali land in Haixing County and put it into 10 mL of sterile saline, and shake it with a vortex instrument for 1 min to prepare a rhizosphere soil bacterial suspension. The bacterial suspension was serially diluted, spread on LB plates containing 5% NaCl, and cultured in an incubator at 30°C for 48 hours to isolate strains.

[0048] 2. Morphological characteristics of the strain

[0049] On the LB plate, a round, smooth and slightly wrinkled, milky white colony is formed. The LB liquid medium grows turbidly, and a bacterial film will be formed in the later stage of the culture. After Gram staining, Gram-positive bacilli can be seen under the microscope, with spores.

[0050] 3. Physiological and biochemical characteristics of the strain

[0051] The strain can grow on LB plates with a salt content of 5%. After th...

Embodiment 3

[0057] Example 3 Preparation of biological fertilizer containing Bacillus amyloliquefaciens ZCM18 and Bacillus tequila ZCM5

[0058] The seed medium of Bacillus amyloliquefaciens ZCM18 is LB medium, inoculate the bacterium solution stored in glycerol tubes on LB solid medium for activation, culture at 35°C for 2 days, and inoculate a single colony into LB liquid medium, at 35°C, 200r / min, culture 15h to the late logarithmic period, and the bacterial liquid obtained after the inoculation amount of 1% is transferred once more is the seed liquid. Fermentation of ZCM18 was carried out with this seed liquid. Usually at 35°C, 200r / min, culture for 48h, the number of bacteria ≥ 1×10 10 cfu / mL.

[0059] The seed medium of Bacillus tequila ZCM5 is LB medium, inoculate the bacterial solution stored in glycerol tubes on LB solid medium for activation, culture at 30°C for 2 days, inoculate a single colony into LB liquid medium, and inoculate at 30°C , 200r / min, culture 15h to the late ...

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Abstract

The invention discloses a growth-promoting bacteria combination for enhancing salt tolerance of crops in moderate-severe saline and alkaline lands. The growth-promoting bacteria combination comprises a bacillus amyloliquefaciens strain ZCM18 and a bacillus tequilensis strain. Preservation numbers of the two strains are CGMCC No.1295 and CGMCC No.12960 respectively. The two strains are capable of tolerate the salinity of 5%, have high capabilities of phosphate solving, nitrogen fixing and producing IAA (indoleacetic acid), ACCD (acetyl-CoA carboxylase subunit D), siderophore and cytokinin, and can antagonize root pathogenic bacteria including fusarium, rhizoctonia, pythium and the like. The two stains after being combined are suitable for the moderate-severe saline and alkaline lands (with salt content ranging from 0.4% to 1.0%), and in the production field of biological fertilizer for salt tolerating and growth benefiting for the crops, the growth-promoting bacteria combination is promising in prospects of crop's salt tolerance enhancement, crop root growth benefiting, seedling existing rate increasing, fertilizer using amount decreasing and crop yield increasing.

Description

technical field [0001] The invention relates to the field of microbial growth promotion and plant protection, in particular to a combination of growth-promoting bacteria for improving the salt tolerance of crops in medium-to-severe saline-alkali lands. Background technique [0002] The improvement and utilization of saline-alkali land is a hotspot and difficulty in international research. At present, technologies such as hydraulic salt improvement, physical adsorption improvement, salt water drainage, and slag adsorption have been developed, but they are not suitable for arid and semi-arid areas where fresh water resources are scarce. In the rhizosphere of saline-alkali-tolerant plants, there are a large number of beneficial microbial resources. Some strains have high salt-tolerant ability, and can significantly improve the salt-tolerant ability of plants, and have the functions of promoting growth, disease prevention, and anti-aging. Application In the rhizosphere of plants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 王占武张翠绵彭杰丽胡栋贾楠黄晓东
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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