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36 results about "Nicotine tobacco" patented technology

Enhanced Delivery of Nicotine, THC, Tobacco, Cannabidiol or Base Alkaloid from an Electronic Cigarette or Other Vapor or Smoke Producing Device Through Use of an Absorption Conditioning Unit

A method for the administration of nicotine, THC, tobacco, cannabidiol or a base alkaloid includes administering in the oral or nasal cavity an absorption conditioning unit having at least two agents selected from the group consisting of (a) a buffer agent, (b) a capturing agent, (c) a penetration agent, and (d) a thermal agent, to the mammal, and then administering by inhalation a bioactive agent selected from the group consisting of nicotine, THC, cannabidiol and a base alkaloid. The absorption conditioning unit may be in a dosage form not containing a drug. The absorption conditioning unit may create a pH in the oral cavity or nasal cavity of 7.8-10 for a period of ten minutes or more after administration, the dosage form not containing an acid and not containing a drug.
Owner:FUISZ RICHARD C +1

Enhanced Delivery of Nicotine, THC, Tobacco, Cannabidiol or Base Alkaloid from an Electronic Cigarette or Other Vapor Producing Device Through Use of an Absorption Conditioning Unit

A method for the administration of nicotine, THC, tobacco, cannabidiol or a base alkaloid includes administering an absorption conditioning unit having at least two agents selected from the group consisting of (a) a buffer agent, (b) a capturing agent, (c) a penetration agent, and (d) a thermal agent, to the mammal, and then administering by inhalation a bioactive agent selected from the group consisting of nicotine, THC, cannabidiol and a base alkaloid. The absorption conditioning unit may be in a dosage form not containing a drug. The absorption conditioning unit may create a pH in the oral cavity of 7.8-10 for a period of ten minutes or more after administration, the dosage form not containing an acid and not containing a drug.
Owner:FUISZ RICHARD C +1

Reduced-exposure tobacco products

The present invention provides methodologies, including the utilization of genetically modified (GM) tobaccos, for the development and production of consumer acceptable PREPs encompassing the following: 1) production of low tar-to-nicotine ratio cigarettes, which allow smokers to obtain satisfying amounts of nicotine more efficiently than conventional cigarettes while reducing whole smoke deliveries; thereby reducing smoker “compensation” that exists with conventional low-yield cigarettes; 2) reduction of harmful tobacco-specific nitrosamines in tobacco products by genetic means and by extracting nicotine from tobacco and combining such with genetically modified reduced-nicotine tobacco; 3) production of improved expanded tobacco which utilizes genetically modified increased-nicotine tobacco; and 4) production of reconstituted tobacco which includes any combinations of the following: genetically modified increased-nicotine tobacco, genetically modified reduced-nicotine tobacco, tobacco leaf fractions, and freshly harvested freeze-dried tobacco.
Owner:22ND CENTURY LTD LLC

Preparation method of high-nicotine tobacco extract

The invention discloses a preparation method of a high-nicotine tobacco extract. The preparation method of the high-nicotine tobacco extract belongs to the field of tobacco additives, and mainly comprises three steps of raw material pretreatment, subcritical extraction and molecular distillation. A baking process is adopted for pretreating tobacco leaves, so that reducing sugar of tobacco and a nitrogen-containing compound are promoted to generate maillard reaction, substances such as pyrone and furanone are produced, the baking aroma is improved, the reducing sugar content is reduced, the suction offensive odor is reduced, meanwhile, combined-state nicotine in the tobacco leaves is converted into a free state, and the content of the free-state nicotine is improved. A weak polar solvent is adopted for subcritical extraction, so that the extraction rate of tobacco feature aroma substances and the free-state nicotine can be improved; and low-temperature molecular distillation is combined, so that low-boiling-point aroma substances and the nicotine can be effectively enriched, the damage on thermosensitive substances due to high temperature is avoided, and meanwhile, the content of macromolecular substances such as pectin and protein in an extract is reduced. The method is simple in preparation process, simple and convenient to operate, and suitable for industrial production and application.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

High-nicotine tobacco extract and application thereof

ActiveCN106387990ACater to physiological needsIncrease contentTobacco treatmentNicotiana tabacumDamascone
The invention discloses a high-nicotine tobacco extract and application thereof, and belongs to the field of tobacco additives. The extract is rich in aroma components such as megastigmatrienone, damascone, pyrones and furanones in tobacco, can provide tobacco aroma and roasting aroma, further contains certain nicotine, can cater for physiological needs of consumers, and improves satisfaction. Content of macromolecular substances such as proteins, pectin and polysaccharides in the extract is extremely low, solubleness is good, the high-nicotine tobacco extract can be completely dissolved into MCT (medium chain triglyceride), and can be used for perfuming a filter tip. Besides, the high-nicotine tobacco extract is added into cut stems or reconstituted tobaccos, so that stimulus can be reduced, strength is improved, a blending rate, in a tobacco group, of the cut stems or reconstituted tobaccos is increased, and content of cigarette tar is reduced while fragrance is invigorated and impurities are covered.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

E-cigarette nicotine liquid and preparation method thereof

The invention discloses E-cigarette nicotine liquid and a preparation method thereof and belongs to the technical field of E-cigarette nicotine liquids. The E-cigarette nicotine liquid is made from high-nicotine tobacco extract, tobacco flavor and the like, wherein the tobacco extract has rich tobacco characteristic fragrant ingredients (such as megastigmatrienone, beta-damascone, pyrones, and furanones) and nicotine, can provide tobacco aroma and curing aroma and meet the physiological needs of consumers, and provides better satisfaction. The high-nicotine tobacco extract has very low contents of high-boiling-point ingredients and macromolecular substances (such as proteins, pectin, and polysaccharides), is free of burnt smell and low in sweetness, and can supplement aroma and cover peculiar odors, lower stimulus and improve taste strength. By being used with tobacco flavors, the E-cigarette nicotine liquid has enhanced curing aroma and tobacco aroma, and has strong aroma and good volatility. The preparation process of the E-cigarette nicotine liquid is simple, is simple and easy to perform, and is applicable to large-scale industrial production and application.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Method for early-stage screening of high-nicotine and low-nicotine tobacco converter plants

The invention relates to a method for early-stage screening of high-nicotine and low-nicotine tobacco converter plants. The method mainly comprises the steps of taking aseptic seedling leaves from indoor tobacco seed sprout, adopting a CTAB small-sample method to extract tobacco genome DNA, using the DNA as a template, using a nicotine converted specific primer to perform PCR amplification, adopting 2% agarose gel electrophoresis to separate and show a PCR amplification product, observing, reading and counting molecular marker band spectrums in a gel-imaging system and comparing the band spectrums with a contrast characteristic band spectrum to screen high-nicotine and low-nicotine conversion materials. The method is not limited by environment conditions and seasonal influence, does not need artificial chemical inducted tobacco conversion, high-nicotine and low-nicotine converter plant screening is performed before sowing, and time cost and economic cost are greatly saved. In addition, the method is accurate, efficient, simple and convenient to operate and easy to centralize and popularize, can be used for screening of high-nicotine and low-nicotine conversion materials of different types of tobaccos and can be also used for quick purification and identification performed according to specific nicotine conversion characters.
Owner:HUBEI TOBACCO SCI RES INST

Method for rapidly screening high-nicotine tobacco mutants

The invention discloses a method for rapidly screening high-nicotine tobacco mutants, and belongs to the biotechnical field. The method comprises the following steps: 1) dibbling tobacco M2-generation seeds onto an aqueous methyl jasmonate solution; 2) 15 days later, screening single plants of which root lengths are significantly suppressed; 3) transferring the single plants screened in the step 2) to an MS culture solution, and culturing for 20 days; 4) transferring the tobacco plants in the step 3) to a provisonal planting plate containing nutrient soil, and 30 days later, transplanting to a field; 5) performing field planting and routine management; 6) determinating nicotine contents at three different time points; 7) selfing and harvesting seeds. According to the method, within 20 days, high-nicotine materials are screened from 4000 China tobacco-100 mutants, and 5 high-nicotine flue-cured tobacco materials are obtained; the highest nicotine content reaches 7.09%, which is the highest nicotine content for current flue-cured tobacco; the efficiency for screening the tobacco mutants is greatly improved, the workload for screening the high-nicotine tobacco is reduced, and the production efficiency is improved.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD

Low-nicotine tobacco extract and preparation method thereof

The invention discloses a low-nicotine tobacco extract and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing tobacco extract liquid with relatively low nicotine content by extracting through a ketone or ester solvent; secondly, removing a certain amount of nicotine by water washing; thirdly, concentrating for removing the solvent; finally, completely removing the nicotine through molecular distilling to obtain a tobacco extract spice which is very low in nicotine content and higher in fragrance quality. By adopting the preparation method, the treated tobacco extract can be added into cigarettes in a larger amount, the cigarette fragrance can be improved more remarkably, the fragrance and concentration are enhanced, the strength and irritation are not increased basically, and the cigarette quality is improved well.
Owner:CHINA TOBACCO ANHUI IND CO LTD

Liquid sea salt nicotine and preparation method thereof

ActiveCN111466608AGreat tasteReduce overstimulationTobacco treatmentLiquid smokeGlycerol
The invention discloses liquid sea salt nicotine. The formula of the liquid sea salt nicotine comprises, by mass, the following components: 1, 250 to 350 parts of basic organic acid; 100 to 150 partsof alcohol-soluble organic acid; 350-450 parts of nicotine, 100-150 parts of absolute ethyl alcohol, 100-150 parts of sea salt, 100-150 parts of distilled water, 10-20 parts of menthol, 10-20 parts ofxylitol, 2-5 parts of natural sweetening agent, 50-70 parts of propylene glycol, 50-70 parts of glycerol, 1-3 parts of mint extracting solution and the like. The sea salt, the sweetening agent and various natural plant extracting solutions are added into tobacco tar, the taste of the tobacco tar smoked by a smoker can be improved, and therefore excessive stimulation of nicotine tobacco juice to the throat is reduced; citric acid, ascorbic acid and the like are added into organic acid components of the sea salt nicotine, the alcohol-soluble organic acid can increase the concentration of smoke,the basic organic acid and an acid-base value of the alcohol-soluble organic acid are matched to adjust the whole acid-base value, the smoking experience of the tobacco tar can be improved through the added natural plant extract, the smoker is assisted in relieving smoking addiction, and safety is high.
Owner:SHENZHEN YIYOU INT TECH

Method for screening high-nicotine tobacco variety mutants

InactiveCN111448983APlant genotype modificationBiotechnologyNicotine synthesis
The invention relates to a method for screening high-nicotine tobacco variety mutants. According to the method, mutagenized plants are created by the EMS mutagenesis method, leaf glandular hair morphology screening and leaf secretion determination are performed to select first mutant strains meeting first set criteria, and M3 generation seeds are harvested for cultivation; the Tilling technology is used to screen mutant single plants of a nicotine synthesis negative regulation gene NtJAZ1, continuous backcross and self-cross are carried out between the mutant single plants and tobacco varieties to be screened as recurrent parents, and mutant strains with high-nicotine and high-secretion are obtained with the help of the molecular marker and gene chip-assisted selection technology; and comparative studies on agronomic traits, comprehensive resistance, chemical composition, sensory quality and the like of the mutant strains are performed, new varieties with increased nicotine content, excellent comprehensive traits, and significantly-improved tobacco aroma are selected from the mutant strains, and thereby the foundation for the production of highly-flavored high-quality tobaccos is laid.
Owner:CHINA TOBACCO HENAN IND

Low-nicotine tobacco extract and preparation method thereof and tobacco tar special for heat-not-burn cigarette

The invention provides a low-nicotine tobacco extract and a preparation method thereof and tobacco tar special for a heat-not-burn cigarette. The preparation method of the low-nicotine tobacco extractcomprises the following steps of: extraction, specifically, adopting an ethanol solution for conducting digestion on tobacco raw materials, performing solid-liquid separation after digestion to obtain leach liquor, and centrifuging the leach liquor to obtain a tobacco extracting solution; concentrating: concentrating the tobacco extracting solution under reduced pressure to obtain a tobacco alcohol extract; alkalization extraction: adding ammonia water into the tobacco alcohol extract, performing uniform stirring, and mixing with an organic solvent for extraction; purification: separating liquid after extraction to obtain an organic phase and a water phase, and mixing the water phase with an ethanol solution, performing uniform stirring, and performing concentrating under reduced pressureto obtain the low-nicotine tobacco extract. The preparation method disclosed by the invention is simple in process requirement, high in yield and suitable for large-batch production. The tobacco extract prepared by adopting the method still has obvious tobacco fragrance while having lower nicotine content, and can be used for preparing heat-not-burn cigarettes.
Owner:贵州黄果树金叶科技有限公司

Method for rapidly obtaining high-nicotine tobacco mutant by screening tobacco root systems

InactiveCN110393147AAvoiding problems with mutating materialsKill two birds with one stonePlant genotype modificationNicotiana tabacumMutant
The invention provides a method for rapidly obtaining a high-nicotine tobacco mutant by screening tobacco root systems. Yunyan 87 mutation second-generation seeds and Yunyan 87 control seeds are subjected to spot seeding respectively, after the Yunyan 87 control seeds grow out two true leaves, Yunyan 87 mutation second-generation materials and Yunyan 87 control material seedlings are pulled out and cleaned to compare the root systems, and a mutant material KH65 with the more developed root systems is screened out and cultured and transplanted; after tobacco plants are mature, upper, middle andlower tobacco leaves are sampled to measure the nicotine content of a single plant KH65; after the tobacco plant KH65 blooms, selfing is conducted to collect seeds, and the seeds are sown again in the second year; the root system conditions of the mutant material are identified again, and after the tobacco plant is mature, sampling is conducted again to measure the nicotine content of the maturemutant material of the single plant; after the mature mutant tobacco plant grow to bloom, selfing is conducted to collect seeds, and the high-nicotine tobacco mutant is obtained. According to the method, the tobacco mutant material high in nicotine content can be rapidly identified and obtained by screening the root expression of the tobacco EMS mutant material.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI

Oral pharmaceutical composition with plant alkaloid for treatment of dependencies

This invention is related to an oral pharmaceutical composition that contains a cholinergic agent, a natural plant alkaloid in particular, selected from the group of lobeline, anabasine, cytisine, galantamine or their acceptable salts, in the form of tablets and capsules. The excipients of the developed oral composition include cellulose powder, calcium sulphate, silica colloidal and magnesium stearate, the total content of cellulose powder and calcium sulphate dihydrate being from 64,5 to 97,5 % of the mass of the dosage form and at least 90% of the alkaloid particles being < 100um. The oral composition contains also at least one biologically active amino acid selected from: L-carnitine, tryptophan or a combination of them. The oral composition according to this invention achieves uniform distribution of the active substance in the composition, as well as stability of the composition due to the included excipients selected so as to react to a minimum with the alkaloid to form the qualitative and quantitative related substances admissible for the pharmaceutical composition. The composition according to this invention is applicable in the treatment of dependency and addiction to nicotine, tobacco products and alcohol.
Owner:索非药剂有限公司

Tobacco nicotine metabolism related gene and application thereof

The invention discloses a tobacco nicotine metabolism related gene and application thereof. The gene is an NtLNP2 gene, the sequence of the gene is SEQ ID No.1, and after the sequence of the gene is translated, the coded protein sequence is SEQ ID No.2. A method for knocking out the tobacco nicotine metabolism related gene by using a CRISPR / Cas9 system comprises the following steps: (1) designing an sgRNA guide sequence, and constructing an sgRNA expression vector; (2) introducing the expression vector into agrobacterium; (3) infecting the callus; and (4) carrying out a detection test on the nicotine content of the squaring-stage leaves of the NtLNP2 gene homozygous knockout material by GC-MS. The gene and the method provided by the invention provide genetic materials and theoretical basis for tobacco nicotine metabolism gene function research and low-nicotine tobacco new variety cultivation research.
Owner:CHINA TOBACCO YUNNAN IND

A Rapid Screening Method for High Nicotine Tobacco Mutants

The invention discloses a method for rapidly screening high-nicotine tobacco mutants, and belongs to the biotechnical field. The method comprises the following steps: 1) dibbling tobacco M2-generation seeds onto an aqueous methyl jasmonate solution; 2) 15 days later, screening single plants of which root lengths are significantly suppressed; 3) transferring the single plants screened in the step 2) to an MS culture solution, and culturing for 20 days; 4) transferring the tobacco plants in the step 3) to a provisonal planting plate containing nutrient soil, and 30 days later, transplanting to a field; 5) performing field planting and routine management; 6) determinating nicotine contents at three different time points; 7) selfing and harvesting seeds. According to the method, within 20 days, high-nicotine materials are screened from 4000 China tobacco-100 mutants, and 5 high-nicotine flue-cured tobacco materials are obtained; the highest nicotine content reaches 7.09%, which is the highest nicotine content for current flue-cured tobacco; the efficiency for screening the tobacco mutants is greatly improved, the workload for screening the high-nicotine tobacco is reduced, and the production efficiency is improved.
Owner:TOBACCO RES INST CHIN AGRI SCI ACAD

Method for creating low-nicotine tobacco mutant by knocking out NtBBLs by using CRISPR/Cas9 and application of low-nicotine tobacco mutant

The invention discloses a method for creating a low-nicotine tobacco mutant by knocking out NtBBLs by using CRISPR / Cas9 and application. The method comprises the following steps: (1) constructing a CRISPR / Cas9 gene editing carrier; (2) preparing infected agrobacterium; (3) carrying out genetic transformation to obtain regenerated edited plants and homozygous T1-generation plants; and (4) carrying out a detection test on the nicotine content of leaves of the NtBBLs gene homozygous knockout material in the squaring stage by GC-MS (Gas Chromatography-Mass Spectrometer). According to the application, the NtBBLs gene is knocked out by utilizing a CRISPR / Cas9 mediated gene editing technology, so that a tobacco knockout material which keeps original agronomic characters and meanwhile, the nicotine content is greatly reduced can be obtained.
Owner:CHINA TOBACCO YUNNAN IND

Preparation method of high-aroma low-nicotine tobacco shreds

The invention discloses a preparation method of high-aroma low-nicotine tobacco shreds. The preparation method comprises the following steps: extracting tobacco shreds with a non-polar solvent, a weak-polar solvent, a medium-polar solvent and a polar solvent in sequence, removing solvents from extract solutions of the non-polar solvent, the weak-polar solvent and the medium-polar solvent, respectively backfilling the extracted tobacco shreds with the extract solutions, and performing cut tobacco drying / fluidized drying to obtain the high-aroma low-nicotine tobacco shreds. According to the method, the aroma quality of the tobacco shreds is ensured while the nicotine content is reduced.
Owner:CHINA TOBACCO YUNNAN IND +2

A kind of electronic cigarette smoke liquid and preparation method thereof

ActiveCN106617265BIncreased roasted aromaIncrease the aroma of tobaccoTobacco treatmentLiquid smokeAdditive ingredient
The invention discloses E-cigarette nicotine liquid and a preparation method thereof and belongs to the technical field of E-cigarette nicotine liquids. The E-cigarette nicotine liquid is made from high-nicotine tobacco extract, tobacco flavor and the like, wherein the tobacco extract has rich tobacco characteristic fragrant ingredients (such as megastigmatrienone, beta-damascone, pyrones, and furanones) and nicotine, can provide tobacco aroma and curing aroma and meet the physiological needs of consumers, and provides better satisfaction. The high-nicotine tobacco extract has very low contents of high-boiling-point ingredients and macromolecular substances (such as proteins, pectin, and polysaccharides), is free of burnt smell and low in sweetness, and can supplement aroma and cover peculiar odors, lower stimulus and improve taste strength. By being used with tobacco flavors, the E-cigarette nicotine liquid has enhanced curing aroma and tobacco aroma, and has strong aroma and good volatility. The preparation process of the E-cigarette nicotine liquid is simple, is simple and easy to perform, and is applicable to large-scale industrial production and application.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

A kind of preparation method of high nicotine tobacco extract

The invention discloses a preparation method of a high-nicotine tobacco extract. The preparation method of the high-nicotine tobacco extract belongs to the field of tobacco additives, and mainly comprises three steps of raw material pretreatment, subcritical extraction and molecular distillation. A baking process is adopted for pretreating tobacco leaves, so that reducing sugar of tobacco and a nitrogen-containing compound are promoted to generate maillard reaction, substances such as pyrone and furanone are produced, the baking aroma is improved, the reducing sugar content is reduced, the suction offensive odor is reduced, meanwhile, combined-state nicotine in the tobacco leaves is converted into a free state, and the content of the free-state nicotine is improved. A weak polar solvent is adopted for subcritical extraction, so that the extraction rate of tobacco feature aroma substances and the free-state nicotine can be improved; and low-temperature molecular distillation is combined, so that low-boiling-point aroma substances and the nicotine can be effectively enriched, the damage on thermosensitive substances due to high temperature is avoided, and meanwhile, the content of macromolecular substances such as pectin and protein in an extract is reduced. The method is simple in preparation process, simple and convenient to operate, and suitable for industrial production and application.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Electronic cigarette appliance

The invention belongs to the technical field of electronic cigarettes, and relates to an electronic cigarette appliance. The electronic cigarette appliance comprises a shell; at least two atomizers are arranged in the shell; and each atomizer corresponds to one tar storage bin. According to the invention, (PG / VG + nicotine) and (PG / VG + a certain essence) are separated, so that nicotine and taste additive tobacco tar are simultaneously separated and atomized, nicotine tobacco tar and essence tobacco tar do not need to be mixed, heated and atomized, and the toxic effect generated by mixing and heating chemical substances and the synergistic or antagonistic effect possibly generated on addiction or other nervous system effects are relieved.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI

High-nicotine tobacco extract, preparation method thereof and heat-not-burn cigarette

PendingCN112011405AHigh nicotineHigh nicotine contentTobacco treatmentEssential-oils/perfumesBiotechnologyOrganic solvent
The invention provides a high-nicotine tobacco extract, a preparation method thereof and a heat-not-burn cigarette, and the preparation method of the high-nicotine tobacco extract comprises the following steps: mixing crushed tobacco raw materials with water, and adding an enzyme preparation for treatment; mixing the treated tobacco raw material with a high-concentration ethanol solution for leaching, performing solid-liquid separation after leaching to obtain a leaching solution, and filtering the leaching solution to obtain a tobacco clear solution; concentrating the tobacco clear liquid under reduced pressure to obtain a tobacco alcohol extract; adding alkali liquor into the tobacco alcohol extract, uniformly stirring, and mixing with an organic solvent for extraction; separating liquidafter extraction to obtain an organic phase and a water phase, and concentrating the organic phase under reduced pressure to obtain a product, namely the high-nicotine tobacco extract. The nicotine content of the prepared high-nicotine tobacco extract is higher than that of a conventional tobacco extract, and the high-nicotine tobacco extract has obvious tobacco fragrance and strength. The heat-not-burn cigarette added with a small amount of high-nicotine tobacco extract is rich in tobacco fragrance and strength.
Owner:广东多利宝生物科技有限公司

A kind of low-nicotine tobacco extract, its preparation method and e-liquid special for heat-not-burn cigarettes

The invention provides a low-nicotine tobacco extract, its preparation method and e-liquid special for heat-not-burn cigarettes. The preparation method of the low-nicotine tobacco extract comprises the following steps: Extraction: using ethanol solution to leach tobacco raw materials, extracting After solid-liquid separation to obtain the extract, centrifuge the extract to obtain tobacco extract; Concentration: Concentrate the tobacco extract under reduced pressure to obtain tobacco alcohol extract; Alkaline extraction: Add ammonia water to the tobacco alcohol extract, stir evenly , mixed with an organic solvent for extraction; purification: separation after extraction to obtain an organic phase and an aqueous phase; mixing the aqueous phase with an ethanol solution, stirring evenly, and concentrating under reduced pressure to obtain a low-nicotine tobacco extract. The preparation method of the invention has simple technological requirements, high yield and is suitable for mass production. The tobacco extract prepared by the method has a low nicotine content and still has obvious tobacco aroma, and can be used in the preparation of heat-not-burn cigarettes.
Owner:贵州黄果树金叶科技有限公司
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