Method for screening high-nicotine tobacco variety mutants

Abstract

The invention relates to a method for screening high-nicotine tobacco variety mutants. According to the method, mutagenized plants are created by the EMS mutagenesis method, leaf glandular hair morphology screening and leaf secretion determination are performed to select first mutant strains meeting first set criteria, and M3 generation seeds are harvested for cultivation; the Tilling technology is used to screen mutant single plants of a nicotine synthesis negative regulation gene NtJAZ1, continuous backcross and self-cross are carried out between the mutant single plants and tobacco varieties to be screened as recurrent parents, and mutant strains with high-nicotine and high-secretion are obtained with the help of the molecular marker and gene chip-assisted selection technology; and comparative studies on agronomic traits, comprehensive resistance, chemical composition, sensory quality and the like of the mutant strains are performed, new varieties with increased nicotine content, excellent comprehensive traits, and significantly-improved tobacco aroma are selected from the mutant strains, and thereby the foundation for the production of highly-flavored high-quality tobaccos is laid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and belongs to the cultivation technology of new tobacco varieties, in particular to a method for screening mutants of high-nicotine and high-secretion tobacco varieties. Background technique [0002] Nicotine content is one of the important factors to measure the quality of tobacco leaves, which directly affects the physiological strength, smoke characteristics and safety of tobacco products. Nicotine is the source of the taste of tobacco leaves, which can stimulate the smokers' sense of taste and enable them to obtain physiological satisfaction. At the same time, nicotine also has an important impact on the aroma quality of tobacco leaves. Pyrolysis of nicotine can produce aroma components of pyridine compounds in smoke. Within a certain range, appropriately increasing the nicotine content can endow tobacco leaves with better physiological strength and aroma quality. The nicotine content in tobacc...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for screening mutants of high-nicotine and high-secretion tobacco varieties, so as to solve the problems of traditional cross-breeding due to trait linkage and long cultivation time, and also to solve the uncertain safety of transgenes. Consumers have problems such as psychological barriers

Images